vestnik-bio-msuВестник Московского университета. Серия 16. БиологияVestnik Moskovskogo universiteta. Seriya 16. Biologiya0137-0952Lomonosov Moscow State University, School of Biologyvestnik-bio-msu-1056Research ArticleОРИГИНАЛЬНОЕ ИССЛЕДОВАНИЕRESEARCH ARTICLEВлияние фитогормонов на экспрессию генов USP в проростках Arabidopsis thalianaThe effect of phytohormones on the expression of USP encoding genes in Arabidopsis thaliana seedlingshttps://orcid.org/0000-0002-1663-2710ГоршковаД. С.GorshkovaD. S.

Горшкова Дарья Сергеевна – аспирант, мл. науч. сотр. кафедры физиологии растений биологического факультета; мл. науч. сотр. лаборатории экспрессии генома

119234, г. Москва, Ленинские горы, д. 1, стр. 12;

127276, г. Москва, ул. Ботаническая, д. 35

Тел.: 8-499-678-54-25

Department of Plant Physiology, School of Biology

Leninskiye gory 1–12, Moscow, 119234

Botanicheskaya st. 35, Moscow, 127276

https://orcid.org/0000-0002-5106-5707ПожидаеваЕ. С.PojidaevaE. S.

Пожидаева Елена Станиславовна – канд. биол. наук, ст. науч. сотр. лаборатории экспрессии генома

127276, г. Москва, ул. Ботаническая, д. 35

Тел.: 8-499-678-54-25

Botanicheskaya st. 35, Moscow, 127276

Московский государственный университет имени М.В. Ломоносова; Институт физиологии растений имени К.А. Тимирязева, Российская академия наукРоссияLomonosov Moscow State University; Timiryasev Institute of Plant Physiology, Russian Academy of SciencesRussian FederationИнститут физиологии растений имени К.А. Тимирязева, Российская академия наукРоссияTimiryasev Institute of Plant Physiology, Russian Academy of SciencesRussian Federation202101122021764241249Copyright © Горшкова Д.С., Пожидаева Е.С., 20212021Горшкова Д.С., Пожидаева Е.С.Gorshkova D.S., Pojidaeva E.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestnik-bio-msu.elpub.ru/jour/article/view/1056

Универсальные стрессовые белки (Universal Stress Proteins, USP) являются потенциальными участниками процессов, контролирующих морфогенез растений, в которых важная роль отводится фитогормонам. В данном исследовании был выполнен поиск генов USP Arabidopsis thaliana, изменяющих свою экспрессию в ответ на действие фитогормонов. Выявлены 15 генов USP, экспрессия которых дифференциально регулируется двумя и более фитогормонами. Причем, накопление транскриптов у большинства из исследованных генов наблюдали при действии гормонов, участвующих в формировании устойчивости растений к стрессу – абсцизовой кислоты, этилена и метилжасмоната. В то же время ауксины и гиббереллины – гормоны, регулирующие рост растений, – подавляли экспрессию изученных генов USP. Полученные результаты выявили потенциальные гены USP, чья функциональная активность напрямую или опосредовано может быть связана с фитогормон-зависимыми процессами, обеспечивающими жизнедеятельность растений в нормальных и стрессовых условиях.

Universal stress proteins (USP) are potentially involved in the processes that control plant morphogenesis, in which phytohormones play an important role. In this study, we searched for the genes of Arabidopsis thaliana USP that modulate their expression in response to the action of phytohormones. Fifteen USP genes have been identified whose expression is differently regulated by two or more phytohormones. Moreover, the accumulation of transcripts of the most studied genes was observed under the action of hormones involved in the formation of plant resistance to stress – abscisic acid, ethylene, and methyl jasmonate. At the same time, auxins and gibberellins, hormones that regulate plant growth, suppressed the expression of the studied USP genes. The obtained results revealed potential genes of USP, whose functional activity may be directly or indirectly associated with phytohormone-dependent processes that ensure plant growth under normal and stress conditions.

абсцизовая кислотаауксиныгиббереллиныметилжасмонатуниверсальные стрессовые белкиэтиленArabidopsis thalianaGRUSPAbscisic acidauxinsgibberellinsmethyl jasmonateuniversal stress proteinsethyleneArabidopsis thalianaGRUSPИсследование выполнено при финансовой поддержке Российского фонда фундаментальных исследований в рамках научного проекта № 19-34-90017. Работа осуществлена без использования животных и без привлечения людей в качестве испытуемых. Авторы заявляют об отсутствии конфликта интересов.The research was funded by the Russian Foundation for Basic Research, project no. 19-34-90017.ReferencesKerk D., Bulgrien J., Smith D.W., Gribskov M. Arabidopsis proteins containing similarity to the universal stress protein domain of bacteria // Plant Physiol. 2003. Vol. 131. N 3. P. 1209–1219.Kerk D., Bulgrien J., Smith D.W., Gribskov M. Arabidopsis proteins containing similarity to the universal stress protein domain of bacteria // Plant Physiol. 2003. Vol. 131. N 3. P. 1209–1219.Nyström T., Neidhardt F.C. Expression and role of the universal stress protein, UspA, of Escherichia coli during growth arrest // Mol. Microbiol. 1994. Vol. 11. N 3. P. 537–544.Nyström T., Neidhardt F.C. Expression and role of the universal stress protein, UspA, of Escherichia coli during growth arrest // Mol. Microbiol. 1994. Vol. 11. N 3. P. 537–544.Vollmer A.C., Bark S.J. Twenty-five years of investigating the universal stress protein: function, structure, and applications // Adv. Appl. Microbiol. 2017. Vol. 102. P. 1–36.Vollmer A.C., Bark S.J. Twenty-five years of investigating the universal stress protein: function, structure, and applications // Adv. Appl. Microbiol. 2017. Vol. 102. P. 1–36.Chi Y.H., Koo S.S., Oh H.T., Lee E.S., Park J.H., Phan K.A.T., Wi S.D., Bae S.B., Paeng S.K., Chae H.B., Kang C.H., Kim M.G., Kim W.-Y., Yun D.-J., Lee S.Y. The physiological functions of universal stress proteins and their molecular mechanism to protect plants from environmental stresses // Front. Plant. Sci. 2019. Vol. 10: 750.Chi Y.H., Koo S.S., Oh H.T., Lee E.S., Park J.H., Phan K.A.T., Wi S.D., Bae S.B., Paeng S.K., Chae H.B., Kang C.H., Kim M.G., Kim W.-Y., Yun D.-J., Lee S.Y. The physiological functions of universal stress proteins and their molecular mechanism to protect plants from environmental stresses // Front. Plant. Sci. 2019. Vol. 10: 750.Bhuria M., Goel P., Kumar S., Singh A.K. Genomewide identification and expression profiling of genes encoding universal stress proteins (USP) identify multistress responsive USP genes in Arabidopsis thaliana // Plant Physiol. Rep. 2019. Vol. 24. N 3. P. 434–445.Bhuria M., Goel P., Kumar S., Singh A.K. Genomewide identification and expression profiling of genes encoding universal stress proteins (USP) identify multistress responsive USP genes in Arabidopsis thaliana // Plant Physiol. Rep. 2019. Vol. 24. N 3. P. 434–445.Jung Y.J., Melencion S.M.B., Lee E.S., Park J.H., Alinapon C.V., Oh H.T., Yun D.-J., Chi Y.N., Lee S.Y. Universal stress protein exhibits a redox-dependent chaperone function in Arabidopsis and enhances plant tolerance to heat shock and oxidative stress // Front. Plant. Sci. 2015. Vol. 6: 1141.Jung Y.J., Melencion S.M.B., Lee E.S., Park J.H., Alinapon C.V., Oh H.T., Yun D.-J., Chi Y.N., Lee S.Y. Universal stress protein exhibits a redox-dependent chaperone function in Arabidopsis and enhances plant tolerance to heat shock and oxidative stress // Front. Plant. Sci. 2015. Vol. 6: 1141.Melencion S.M.B., Chi Y.H., Pham T.T., Paeng S.K., Wi S.D., Lee C., Ryu S.W., Koo S.S., Lee S.Y. RNA chaperone function of a universal stress protein in Arabidopsis confers enhanced cold stress tolerance in plants // Int. J. Mol. Sci. 2017. Vol. 18. N 12: 2546.Melencion S.M.B., Chi Y.H., Pham T.T., Paeng S.K., Wi S.D., Lee C., Ryu S.W., Koo S.S., Lee S.Y. RNA chaperone function of a universal stress protein in Arabidopsis confers enhanced cold stress tolerance in plants // Int. J. Mol. Sci. 2017. Vol. 18. N 12: 2546.Verma V., Ravindran P., Kumar P.P. Plant hormonemediated regulation of stress responses // BMC Plant Biol. 2016. Vol. 16: 86.Verma V., Ravindran P., Kumar P.P. Plant hormonemediated regulation of stress responses // BMC Plant Biol. 2016. Vol. 16: 86.Bhuria M., Goel P., Kumar S., Singh A.K. The promoter of AtUSP is co-regulated by phytohormones and abiotic stresses in Arabidopsis thaliana // Front. Plant. Sci. 2016. Vol. 7: 1957.Bhuria M., Goel P., Kumar S., Singh A.K. The promoter of AtUSP is co-regulated by phytohormones and abiotic stresses in Arabidopsis thaliana // Front. Plant. Sci. 2016. Vol. 7: 1957.Gorshkova D.S., Getman I.A., Voronkov A.S., Chizhova S.I., Kuznetsov Vl.V., Pojidaeva E.S. The gene encoding the Universal Stress Protein AtUSP is regulated by phytohormones and involved in seed germination of Arabidopsis thaliana // Dokl. Biochem. Biophys. 2018. Vol. 479. N 1. P. 105–107.Gorshkova D.S., Getman I.A., Voronkov A.S., Chizhova S.I., Kuznetsov Vl.V., Pojidaeva E.S. The gene encoding the Universal Stress Protein AtUSP is regulated by phytohormones and involved in seed germination of Arabidopsis thaliana // Dokl. Biochem. Biophys. 2018. Vol. 479. N 1. P. 105–107.Sauter M., Rzewuski G., Marwedel T., Lorbiecke R. The novel ethylene-regulated gene OsUSP1 from rice encodes a member of a plant protein family related to procaryotic universal stress proteins // J. Exp. Bot. 2002. Vol. 53. N 379. P. 2325–2331.Sauter M., Rzewuski G., Marwedel T., Lorbiecke R. The novel ethylene-regulated gene OsUSP1 from rice encodes a member of a plant protein family related to procaryotic universal stress proteins // J. Exp. Bot. 2002. Vol. 53. N 379. P. 2325–2331.Lee L.Y.C., Hou X., Fang L., Fan S., Kumar P.P., Yu H. STUNTED mediates the control of cell proliferation by GA in Arabidopsis // Development. 2012. Vol. 139. N 9. P. 1568–1576.Lee L.Y.C., Hou X., Fang L., Fan S., Kumar P.P., Yu H. STUNTED mediates the control of cell proliferation by GA in Arabidopsis // Development. 2012. Vol. 139. N 9. P. 1568–1576.Gorshkova D.S., Pojidaeva E.S. Members of the Universal Stress Protein family are indirectly involved in gibberellin-dependent regulation of germination and postgermination growth // Russ. J. Plant Physiol. 2021. Vol. 68. N 3. P. 451–462.Gorshkova D.S., Pojidaeva E.S. Members of the Universal Stress Protein family are indirectly involved in gibberellin-dependent regulation of germination and postgermination growth // Russ. J. Plant Physiol. 2021. Vol. 68. N 3. P. 451–462.Gorshkova D.S., Getman I.A., Sergeeva L.I., Kuznetsov Vl.V., Pojidaeva E.S. GRUSP, an universal stress protein, is involved in gibberellin-dependent induction of flowering in Arabidopsis thaliana // Dokl. Biochem. Biophys. 2021. Vol. 499. N 1. P. 233–237.Gorshkova D.S., Getman I.A., Sergeeva L.I., Kuznetsov Vl.V., Pojidaeva E.S. GRUSP, an universal stress protein, is involved in gibberellin-dependent induction of flowering in Arabidopsis thaliana // Dokl. Biochem. Biophys. 2021. Vol. 499. N 1. P. 233–237.Winter D., Vinegar B., Nahal H., Ammar R., Wilson G.V., Provart N.J. An “Electronic Fluorescent Pictograph” browser for exploring and analyzing large-scale biological data sets // PLoS One. 2007. Vol. 8: 718.Winter D., Vinegar B., Nahal H., Ammar R., Wilson G.V., Provart N.J. An “Electronic Fluorescent Pictograph” browser for exploring and analyzing large-scale biological data sets // PLoS One. 2007. Vol. 8: 718.Zentella R., Zhang Z.-L., Park M., Thomas S.G., Endo A., Murase K., Fleet C.M., Jikumaru Y., Nambara E., Kamiya Y., Sun T.-P. Global analysis of DELLA direct targets in early gibberellin signaling in Arabidopsis // Plant Cell. 2007. Vol. 19. N 10. P. 3037–3057.Zentella R., Zhang Z.-L., Park M., Thomas S.G., Endo A., Murase K., Fleet C.M., Jikumaru Y., Nambara E., Kamiya Y., Sun T.-P. Global analysis of DELLA direct targets in early gibberellin signaling in Arabidopsis // Plant Cell. 2007. Vol. 19. N 10. P. 3037–3057.Uno Y., Furihata T., Abe H., Yoshida R., Shinozaki K., Yamaguchi-Shinozaki K. Arabidopsis basic leucine zipper transcription factors involved in an abscisic acid-dependent signal transduction pathway under drought and high-salinity conditions // Proc. Natl. Acad. Sci. U.S.A. 2000. Vol. 97. N 21. P. 11632–11637.Uno Y., Furihata T., Abe H., Yoshida R., Shinozaki K., Yamaguchi-Shinozaki K. Arabidopsis basic leucine zipper transcription factors involved in an abscisic acid-dependent signal transduction pathway under drought and high-salinity conditions // Proc. Natl. Acad. Sci. U.S.A. 2000. Vol. 97. N 21. P. 11632–11637.Tatematsu K., Kumagai S., Muto H., Sato A., Watahiki M.K., Harper R.M., Liscum E., Yamamotoa K.T. MAS-SUGU2 encodes Aux/IAA19, an auxin-regulated protein that functions together with the transcriptional activator NPH4/ARF7 to regulate differential growth responses of hypocotyl and formation of lateral roots in Arabidopsis thaliana // Plant Cell. 2004. Vol. 16. N 2. P. 379–393.Tatematsu K., Kumagai S., Muto H., Sato A., Watahiki M.K., Harper R.M., Liscum E., Yamamotoa K.T. MAS-SUGU2 encodes Aux/IAA19, an auxin-regulated protein that functions together with the transcriptional activator NPH4/ARF7 to regulate differential growth responses of hypocotyl and formation of lateral roots in Arabidopsis thaliana // Plant Cell. 2004. Vol. 16. N 2. P. 379–393.McGrath K.C., Dombrecht B., Manners J.M., Schenk P.M., Edgar C.I., Maclean D.J., Scheible W.-R., Udvardi M.K., Kazan K. Repressorand activator-type ethylene response factors functioning in jasmonate signaling and disease resistance identified via a genome-wide screen of Arabidopsis transcription factor gene expression // Plant Physiol. 2005. Vol. 139. N 2. P. 949–959.McGrath K.C., Dombrecht B., Manners J.M., Schenk P.M., Edgar C.I., Maclean D.J., Scheible W.-R., Udvardi M.K., Kazan K. Repressorand activator-type ethylene response factors functioning in jasmonate signaling and disease resistance identified via a genome-wide screen of Arabidopsis transcription factor gene expression // Plant Physiol. 2005. Vol. 139. N 2. P. 949–959.Solano R., Stepanova A., Chao Q., Ecker J.R. Nuclear events in ethylene signaling: a transcriptional cascade mediated by ETHYLENE-INSENSITIVE3 and ETHYLENE-RESPONSE-FACTOR1 // Genes Dev. 1998. Vol. 12. N 23. P. 3703–3714.Solano R., Stepanova A., Chao Q., Ecker J.R. Nuclear events in ethylene signaling: a transcriptional cascade mediated by ETHYLENE-INSENSITIVE3 and ETHYLENE-RESPONSE-FACTOR1 // Genes Dev. 1998. Vol. 12. N 23. P. 3703–3714.Corbineau F., Xia Q., Bailly C., El-MaaroufBouteau H. Ethylene, a key factor in the regulation of seed dormancy // Front. Plant Sci. 2014. Vol. 5: 539.Corbineau F., Xia Q., Bailly C., El-MaaroufBouteau H. Ethylene, a key factor in the regulation of seed dormancy // Front. Plant Sci. 2014. Vol. 5: 539.Gepstein S., Sabehi G., Carp M.-J., Hajouj T., Nesher M.F.O., Yariv I., Dor C., Bassani M. Large-scale identification of leaf senescence-associated genes // Plant J. 2003. Vol. 36. N 5. P. 629–642.Gepstein S., Sabehi G., Carp M.-J., Hajouj T., Nesher M.F.O., Yariv I., Dor C., Bassani M. Large-scale identification of leaf senescence-associated genes // Plant J. 2003. Vol. 36. N 5. P. 629–642.Song L., Liu D. Ethylene and plant responses to phosphate deficiency // Front. Plant. Sci. 2015. Vol. 6: 796.Song L., Liu D. Ethylene and plant responses to phosphate deficiency // Front. Plant. Sci. 2015. Vol. 6: 796.Bari R., Pant B.D., Stitt M., Scheible W.-R. PHO2, microRNA399, and PHR1 define a phosphate-signaling pathway in plants // Plant Physiol. 2006. Vol. 141. N 3. P. 988–999.Bari R., Pant B.D., Stitt M., Scheible W.-R. PHO2, microRNA399, and PHR1 define a phosphate-signaling pathway in plants // Plant Physiol. 2006. Vol. 141. N 3. P. 988–999.Khan G.A., Vogiatzaki E., Glauser G., Poirier Y. Phosphate deficiency induces the jasmonate pathway and enhances resistance to insect herbivory // Plant Physiol. 2016. Vol. 171. N 1. P. 632–644.Khan G.A., Vogiatzaki E., Glauser G., Poirier Y. Phosphate deficiency induces the jasmonate pathway and enhances resistance to insect herbivory // Plant Physiol. 2016. Vol. 171. N 1. P. 632–644.Mandaokar A., Dinesh Kumar V., Amway M., Browse J. Microarray and differential display identify genes involved in jasmonate-dependent anther development // Plant Mol. Biol. 2003. Vol. 52. N 4. P. 775–786.Mandaokar A., Dinesh Kumar V., Amway M., Browse J. Microarray and differential display identify genes involved in jasmonate-dependent anther development // Plant Mol. Biol. 2003. Vol. 52. N 4. P. 775–786.Goda H., Sawa S., Asami T., Fujioka S., Shimada Y., Yoshida S. Comprehensive comparison of auxin-regulated and brassinosteroid-regulated genes in Arabidopsis // Plant Physiol. 2004. Vol. 134. N 4. P. 1555–1573.Goda H., Sawa S., Asami T., Fujioka S., Shimada Y., Yoshida S. Comprehensive comparison of auxin-regulated and brassinosteroid-regulated genes in Arabidopsis // Plant Physiol. 2004. Vol. 134. N 4. P. 1555–1573.Nakabayashi K., Okamoto M., Koshiba T., Kamiya Y., Nambara E. Genome-wide profiling of stored mRNA in Arabidopsis thaliana seed germination: epigenetic and genetic regulation of transcription in seed // Plant J. 2005. Vol. 41. N 5. P. 697–709.Nakabayashi K., Okamoto M., Koshiba T., Kamiya Y., Nambara E. Genome-wide profiling of stored mRNA in Arabidopsis thaliana seed germination: epigenetic and genetic regulation of transcription in seed // Plant J. 2005. Vol. 41. N 5. P. 697–709.Reeves W.M., Lynch T.J., Mobin R., Finkelstein R.R. Direct targets of the transcription factors ABAInsensitive(ABI)4 and ABI5 reveal synergistic action by ABI4 and several bZIP ABA response factors // Plant Mol. Biol. 2011. Vol. 75. N 4–5. P. 347–363.Reeves W.M., Lynch T.J., Mobin R., Finkelstein R.R. Direct targets of the transcription factors ABAInsensitive(ABI)4 and ABI5 reveal synergistic action by ABI4 and several bZIP ABA response factors // Plant Mol. Biol. 2011. Vol. 75. N 4–5. P. 347–363.

The authors declare that there are no conflicts of interest present.