vestnik-bio-msuВестник Московского университета. Серия 16. БиологияVestnik Moskovskogo universiteta. Seriya 16. Biologiya0137-0952Lomonosov Moscow State University, School of Biologyvestnik-bio-msu-1057Research ArticleОРИГИНАЛЬНОЕ ИССЛЕДОВАНИЕRESEARCH ARTICLEМорфофункциональная характеристика фибробластов папиллярного и ретикулярного слоев дермы кожи человекаMorphofunctional characteristics of fibroblasts of the papillary and reticular layers of the dermis of human skinhttps://orcid.org/0000-0003-4251-9372РоговаяО. С.RogovayaO. S.

Роговая Ольга Сергеевна – канд. биол. наук, науч. сотр. лаборатории клеточной биологии

119334, г. Москва, ул. Вавилова, д. 26

Тел.: 8-499-135-40-81

Vavilova 26 Moscow, 119334

rogovaya26f@gmail.comhttps://orcid.org/0000-0001-8313-4095ЗупникА. О.ZupnikA. O.

Зупник Андрей Олегович – магистрант кафедры клеточной биологии и гистологии биологического факультета

119234, г. Москва, Ленинские горы, д. 1, стр. 12

Тел: 8-495-939-17-94

Department of Cell Biology and Histology, Faculty of Biology

 

zu.andrey2011@gmail.comhttps://orcid.org/0000-0002-7739-9369ИзмайловаЛ. Ш.IzmailovaL. Sh.

Измайлова Любовь Шамилевна – аспирант лаборатории клеточной биологии 

119334, г. Москва, ул. Вавилова, д. 26

Тел.: 8-499-135-40-81

Vavilova 26 Moscow, 119334

luba.ranaway-94@yandex.ruhttps://orcid.org/0000-0001-5405-0212ВоротелякЕ. А.VorotelyakE. A.

Воротеляк Екатерина Андреевна – докт. биол. наук, чл.-корр. РАН, зав. лабораторией клеточной биологии

119334, г. Москва, ул. Вавилова, д. 26

Тел.: 8-499- 135-40-81

Vavilova 26 Moscow, 119334

vorotelyak@yandex.ruИнститут биологии развития имени Н.К. Кольцова, Российская академия наукРоссияN.K. Koltsov Institute of Developmental Biology, Russian Academy of SciencesRussian FederationМосковский государственный университет имени М.В. ЛомоносоваРоссияLomonosov Moscow State University, Leninskiye gory 1–12, MoscowRussian Federation202101122021764250257Copyright © Роговая О.С., Зупник А.О., Измайлова Л.Ш., Воротеляк Е.А., 20212021Роговая О.С., Зупник А.О., Измайлова Л.Ш., Воротеляк Е.А.Rogovaya O.S., Zupnik A.O., Izmailova L.S., Vorotelyak E.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestnik-bio-msu.elpub.ru/jour/article/view/1057

Фибробласты дермы являются неоднородной популяцией – среди них выделяют несколько субпопуляций, отличающихся происхождением, анатомической региональной специфичностью и расположением в толще дермы. Мы исследовали некоторые фенотипические и функциональные особенности фибробластов папиллярного и ретикулярного слоев дермы кожи человека. Выявлено, что эффективность выделения популяции ретикулярных фибробластов из первичной культуры увеличивается при снижении уровня кислорода до 5%. Морфометрический анализ культивированных фибробластов изученных популяций, сравнение силы и скорости контракции коллагенового геля и данные об уровнях экспрессии CD90 и CD73, полученные методами проточной цитофлуориметрии, показали существенные различия фибробластов папиллярного и ретикулярного слоев дермы человека височной области.

Fibroblasts of the dermis are a heterogeneous population – among them there are several subpopulations that differ in origin, anatomical regional specificity and location in the thickness of the dermis. We investigated some phenotypic and functional features of fibroblasts of the papillary and reticular dermis of human skin. It was found that the introduction of reticular fibroblasts into the primary culture with an oxygen level reduced to 5% increases the efficiency of obtaining this population of fibroblasts. Morphometric analysis of cultured fibroblasts of the studied populations, comparison of the strength and rate of collagen gel contraction, and data on the expression levels of CD90 and CD73 obtained by flow cytometry showed significant differences in fibroblasts of the papillary and reticular layers of the human dermis of the temporal region.

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The authors declare that there are no conflicts of interest present.