vestnik-bio-msuВестник Московского университета. Серия 16. БиологияVestnik Moskovskogo universiteta. Seriya 16. Biologiya0137-0952Lomonosov Moscow State University, School of Biologyvestnik-bio-msu-1131Research ArticleОРИГИНАЛЬНОЕ ИССЛЕДОВАНИЕRESEARCH ARTICLEОсобенности окисления катионов Mn(II) в мембранных препаратах фотосистемы 2 без кислород-выделяющего комплекса. Эволюционный аспектPeculiarities of Mn(II) cation oxidation in the photosystem II without oxygen-evolving complex. Evolutionary aspecthttps://orcid.org/0000-0002-3586-0575ЛовягинаЕ. Р.LovyaginaE. R.

 канд. биол. наук, ст. науч. сотр.

Тел.: 8-495-939-33-15

 Россия, 119234, г. Москва, Ленинские горы, д. 1, стр. 12 

 1–12 Leninskie gory, Moscow, 119234, Russia 

elena.lovyagina@gmail.comhttps://orcid.org/0000-0002-2702-0891ДавлетшинаЛ. Н.DavletshinaL. N.

канд. биол. наук, ст. науч. сотр.

Тел.: 8-495-939-33-15

 Россия, 119234, г. Москва, Ленинские горы, д. 1, стр. 12 

 1–12 Leninskie gory, Moscow, 119234, Russia 

davlet@biophys.msu.ruhttps://orcid.org/0000-0002-0058-2798СеминБ. К.SeminB. K.

докт. биол. наук, вед. науч. сотр.

Тел.: 8-495-939-33-15

 Россия, 119234, г. Москва, Ленинские горы, д. 1, стр. 12 

 1–12 Leninskie gory, Moscow, 119234, Russia 

semin@biophys.msu.ruКафедра биофизики, биологический факультет, Московский государственный университет имени М.В. ЛомоносоваРоссияDepartment of Biophysics, School of Biology, Lomonosov Moscow State UniversityRussian Federation20221406202277298–103Copyright © Ловягина Е.Р., Давлетшина Л.Н., Семин Б.К., 20222022Ловягина Е.Р., Давлетшина Л.Н., Семин Б.К.Lovyagina E.R., Davletshina L.N., Semin B.K.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestnik-bio-msu.elpub.ru/jour/article/view/1131

Одним из основных этапов в процессе трансформации энергии света в оксигенных организмах является извлечение электронов из воды, которые необходимы для световой стадии фотосинтеза. Светозависимое окисление воды осуществляется кислород-выделяющим комплексом (КВК), каталитическим центром которого является кластер Mn4CaO5. КВК расположен в фотосистеме 2 (ФС2) с внутренней стороны тилакоидной мембраны. Эволюционное происхождение ФС2 неясно, так же, как и происхождение марганцевого кластера в КВК. Недавно Джонсон c соавторами (2013) предположили, что в первичной ФС2 в Архейском периоде кластер Mn4CaO5 отсутствовал, а ФС2 окисляла не воду, а катионы Mn(II), т.е. источником электронов были катионы марганца. В представленной работе мы исследовали возможность влияния некоторых факторов окружающей среды (ионы цитрата и пероксид водорода) на скорость окисления катионов Mn(II) ФС2, не содержащей КВК. Мы установили, что цитрат ингибирует окисление катионов марганца, связывая их, но не экстрагирует катионы марганца из КВК. Пероксид водорода, наоборот, значительно увеличивает скорость окисления марганца (с 28±2 до 145±7 мкмоль 2,6-дихлорофенолиндофенола (мг хлорофилла)-1 ∙ ч-1 в присутствии пероксида водорода). Подобные эффекты следует учитывать при исследовании процесса окисления катионов марганца фотосистемой как возможного источника электронов на ранних стадиях эволюции.

One of the main reactions in the process of the light energy transformation in oxygenic organisms is the extraction of electrons from water, which are necessary for the light stage of photosynthesis. The light-dependent oxidation of water is carried out by an oxygen-evolving complex (OEC), the catalytic center of which is the cluster Mn4CaO5. OEC is located in photosystem II (PSII) on the lumen side of thylakoid membrane. The evolutionary origin of the PSII is unclear, as is the origin of the manganese cluster in the OEC. Recently, Johnson et al. (2013) suggested that in the primary PSII in the Archean period, the cluster Mn4CaO5 was absent, and PSII oxidized not water, but Mn(II) cations, i.e., manganese cations were the  source of electrons. In the presented work, we investigated the possibility of the influence of some environmental factors (citrate and hydrogen peroxide) on the oxidation rate of Mn(II) cations by a PSII that does not contain OEC. We found that citrate inhibit the oxidation of Mn(II) cations binding them but doesn’t extract Mn cations from OEC. Hydrogen peroxide, on the contrary, significantly increases the oxidation rate of manganese (from 28±2 to 145±7 μmol 2,6-dichlorophenolindophenol mg Chl-1 h-1 in the presence of H2O2). Such effects should be taken into account when investigating the oxidation of manganese cations by the photosystem as a possible source of electrons in the early stages of evolution. 

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The authors declare that there are no conflicts of interest present.