vestnik-bio-msuВестник Московского университета. Серия 16. БиологияVestnik Moskovskogo universiteta. Seriya 16. Biologiya0137-0952Lomonosov Moscow State University, School of Biologyvestnik-bio-msu-1136Research ArticleКРАТКОЕ СООБЩЕНИЕSHORT COMMUNICATIONОценка спектра протеолитической активности микромицетов рода Aspergillus по отношению к белкам системы гемостазаEvaluation of the spectrum of proteolytic activity of micromycetes of the genus Aspergillus in relation to proteins of the hemostasis systemhttps://orcid.org/0000-0001-6672-0551ОсмоловскийА. А.OsmolovskyA. A.

 канд. биол. наук, доц.

Тел.: 8-495-939-30-33

 Россия, 119234, г. Москва, Ленинские горы, д. 1, стр. 12 

 1–12 Leninskie Gory, Moscow, 119234, Russia 

aosmol@mail.ruШашБ.ŞaşB.

 студент кафедры молекулярной биологии и генетики, факультет естественных наук и литературы

Тел.: +90 542 747 61 10

Ataköy 7-8-9-10, E5 Karayolu Üzeri Ataköy Yerleşkesi, Bakırköy, 34158, Istanbul, Turkey 

Ataköy 7-8-9-10, E5 Karayolu Üzeri Ataköy Yerleşkesi, Bakırköy, 34158, Istanbul, Turkey 

begum.sas99@gmail.comАлександроваА. В.AleksandrovaA. V.

 докт. биол. наук, вед. науч. сотр.

Тел.: 8-495-939-54-82

 Россия, 119234, г. Москва, Ленинские горы, д. 1, стр. 12 

 1–12 Leninskie Gory, Moscow, 119234, Russia 

alinaalex2011@yandex.ruБарановаН. А.BaranovaN. A.

 канд. биол. наук, ст. науч. сотр.

Тел.: 8-495-939-30-33 

 Россия, 119234, г. Москва, Ленинские горы, д. 1, стр. 12 

 1–12 Leninskie Gory, Moscow, 119234, Russia 

baranovana2012@mail.ruКрейерВ. Г.KreyerV. G.

 канд. биол. наук, ст. науч. сотр.

Тел.: 8-495-939-30-33; e-mail: 

 Россия, 119234, г. Москва, Ленинские горы, д. 1, стр. 12 

 1–12 Leninskie Gory, Moscow, 119234, Russia 

vkreyer@yandex.ruКафедра микробиологии, биологический факультет, Московский государственный университет имени М.В. ЛомоносоваРоссияDepartment of Microbiology, Faculty of Biology, Lomonosov Moscow State UniversityRussian FederationСтамбульский университет КюльтюрТурцияDepartment of Molecular Biology and Genetics, Faculty of Natural Sciences and Literature, Istanbul Kültür UniversityTurkeyКафедра микологии и альгологии, биологический факультет, Московский государственный университет имени М.В. ЛомоносоваРоссияDepartment of Mycology and Algology, Faculty of Biology, Lomonosov Moscow State UniversityRussian Federation202215062022772138–144Copyright © Осмоловский А.А., Шаш Б., Александрова А.В., Баранова Н.А., Крейер В.Г., 20222022Осмоловский А.А., Шаш Б., Александрова А.В., Баранова Н.А., Крейер В.Г.Osmolovsky A.A., Şaş B., Aleksandrova A.V., Baranova N.A., Kreyer V.G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestnik-bio-msu.elpub.ru/jour/article/view/1136

Изучена активность внеклеточных протеиназ у семи штаммов разных видов микромицетов рода Aspergillus по отношению к белкам системы гемостаза. Сравнение значений энзиматических индексов при росте штаммов на агаризованных средах с казеином и фибрином с последующим их глубинным культивированием и анализом амидолитической активности с хромогенными пептидными субстратами протеиназ системы гемостаза позволило отобрать штаммы A. candidus A4 и A. crustosus A29 в качестве перспективных продуцентов фибринолитических протеиназ. Образуемые обоими штаммами протеиназы оказались способны расщеплять хромогенные пептидные субстраты тромбина, плазмина, фактора Х, протеина С и урокиназы, проявляя высокую плазминоподобную и тромбиноподобную активность и не обладая активаторным действием к проферментам системы гемостаза.

The activity of extracellular proteinases in seven strains of different species of micromycetes of the genus Aspergillus in relation to proteins of the hemostasis system was studied. Comparison of the values of enzymatic indices during the growth of strains on agar media with casein and fibrin, followed by their submerged cultivation and analysis of amidolytic activity with chromogenic peptide substrates of proteinases of the hemostasis system, made it possible to select strains of A. candidus A4 and A. crustosus A29 as promising producers of fibrinolytic proteinases. The proteinases formed by both strains were able to cleave the chromogenic peptide substrates of thrombin, plasmin, factor X, protein C, and urokinase, exhibiting high plasmin-like and thrombin-like activity and not having an activating effect on the proenzymes of the hemostasis system.

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The authors declare that there are no conflicts of interest present.