vestnik-bio-msuВестник Московского университета. Серия 16. БиологияVestnik Moskovskogo universiteta. Seriya 16. Biologiya0137-0952Lomonosov Moscow State University, School of Biologyvestnik-bio-msu-1161Research ArticleОРИГИНАЛЬНОЕ ИССЛЕДОВАНИЕRESEARCH ARTICLEХарактеристики быстрого натриевого тока в изолированных кардиомиоцитах перепелаCharacteristics of fast sodium current in isolated quail cardiomyocyteshttps://orcid.org/0000-0003-0131-1911ФилатоваТ. С.FilatovaT. S.

Филатова Татьяна Сергеевна – канд. биол. наук, мл. науч. сотр. кафедры физиологии человека и животных биологического факультета

119234, г. Москва, Ленинские горы, д. 1, стр. 12Тел. 8-929-515-25-78

Department of Human and Animal Physiology

1–12 Leninskie gory, Moscow, 119234

filatova@mail.bio.msu.ruhttps://orcid.org/0000-0001-5751-8853АбрамочкинД. В.AbramochkinD. V.

Абрамочкин Денис Валерьевич – докт. биол. наук, вед. науч. сотр. кафедры физиологии человека и животных биологического факультета; зав. лабораторией электрофизиологии сердца

119234, г. Москва, Ленинские горы, д. 1, стр. 12121552, г. Москва, ул. Черепковская 3-я, д. 15аТел. 8-916-603-05-02

Department of Human and Animal PhysiologyLaboratory of Cardiac Electrophysiology

1–12 Leninskie gory, Moscow, 11923415A-10 3rd Cherepkovskaya st., Moscow, 121552

abram340@mail.ruМосковский государственный университет имени М.В. ЛомоносоваРоссияMoscow State UniversityRussian FederationМосковский государственный университет имени М.В. Ломоносова; Национальный медицинский исследовательский центр кардиологииРоссияMoscow State University; National Medical Research Center for CardiologyRussian Federation202220082022773173179Copyright © Филатова Т.С., Абрамочкин Д.В., 20222022Филатова Т.С., Абрамочкин Д.В.Filatova T.S., Abramochkin D.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestnik-bio-msu.elpub.ru/jour/article/view/1161

Быстрый натриевый ток (INa) обеспечивает деполяризацию рабочего миокарда, определяя возбудимость его клеток и скорость распространения возбуждения. Нарушения активации и инактивации каналов натриевого тока могут приводить к развитию различных аритмий. У большинства позвоночных, за исключением млекопитающих, INa исследован достаточно слабо – в том числе и у птиц, представляющих большой интерес для сравнительной физиологии. Данная работа впервые рассматривает характеристики быстрого натриевого тока в миокарде взрослых птиц. С использованием метода пэтч-кламп мы зарегистрировали INa в изолированных предсердных и желудочковых кардиомиоцитах японского перепела. Ток имел бóльшую амплитуду (в сравнении с другими позвоночными) и быстро восстанавливался после инактивации; быстрая временная константа инактивации INa в предсердных миоцитах оказалась меньше, чем в желудочковых. Параметры стационарной активации и инактивации позволяют предположить меньшую выраженность оконного натриевого тока в миокарде птиц по сравнению с таковым в миокарде млекопитающих. В желудочковых кардиомиоцитах перепела блокатор позднего натриевого тока ранолазин вызывал слабое снижение пиковой амплитуды INa и не менял кинетику инактивации, однако сдвигал кривую стационарной активации в сторону более отрицательных потенциалов, укорачивал потенциалы действия и снижал максимальную скорость нарастания их переднего фронта. Таким образом, можно предполагать, что данные характеристики INa в миокарде перепела отражают адаптацию к высокой частоте сокращений сердца птиц и могут указывать на возможные различия в структуре и функционировании натриевых каналов в сердцах птиц и млекопитающих.

Fast sodium current (INa) provides depolarization of working myocardium and defines the excitability of its cells and the velocity of excitation propagation in the tissue. Alterations in activation and inactivation of INa channels can lead to the onset of various arrhythmias. Cardiac INa is poorly studied in most vertebrate animals (excepting mammals) – including birds which are of great interest for comparative physiology. In the present work we for the first time study the characteristics of fast sodium current in myocardium of adult bird. Using standard patch clamp method, we recorded INa in isolated atrial and ventricular cardiomyocytes of Japanese quail. The current had great amplitude and quickly recovered from inactivation both in atrial and ventricular cells; the fast inactivation time constant of INa in atrial cells was lower than that of ventricular cells. Steady-state activation and inactivation suggest that sodium window current in avian myocardium is less pronounced in comparison to that in mammalian heart. In quail ventricular myocytes the blocker of late sodium current ranolazine caused a slight decrease in peak current amplitude and did not affect inactivation – however, it shifted steady-state inactivation curve towards more negative potentials, shortened action potentials and caused a decrease in maximum upstroke velocity. Thus, the characteristics of INa in quail myocardium reflect an adaptation to high heart rates in birds, and also suggest possible differences in the structure and function of INa channels between birds and mammals.

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The authors declare that there are no conflicts of interest present.