vestnik-bio-msuВестник Московского университета. Серия 16. БиологияVestnik Moskovskogo universiteta. Seriya 16. Biologiya0137-0952Lomonosov Moscow State University, School of Biology10.55959/MSU0137-0952-16-78-3-4vestnik-bio-msu-1258Research ArticleОРИГИНАЛЬНОЕ ИССЛЕДОВАНИЕRESEARCH ARTICLEОценка первичных реакций фотосинтеза в индивидуальных клетках микроводорослей микрофлуориметрическим методомEvaluation of the primary reactions of photosynthesis in microalgae single cell by microfluorimetric methodhttps://orcid.org/0000-0002-0414-1236ВолгушеваА. А.VolgushevaА. А.

Волгушева Алена Александровна – канд. биол. наук, ст. науч. сотр. кафедры биофизики биологического факультета МГУ. Тел.: 8-495-939-51-50

119234, г. Москва, Ленинские горы, д. 1, стр. 12

 

Department of Biophysics, Faculty of Biology

1–12 Leninskie gory, Moscow, 119234

volg-alena@yandex.ruhttps://orcid.org/0000-0002-7686-461XКонюховИ. В.KonyukhovI. V.

Конюхов Иван Владимирович – канд. биол. наук, ст. науч. сотр. кафедры биофизики биологического факультета. Тел.: 8-495-939-51-50

119234, г. Москва, Ленинские горы, д. 1, стр. 12

Department of Biophysics, Faculty of Biology

1–12 Leninskie gory, Moscow, 119234

vanka.kon@gmail.comhttps://orcid.org/0000-0002-9690-8034АнталТ. К.AntalT. K.

Антал Тарас Корнелиевич – докт. биол. наук, проректор по научной работе. Тел.: 8-811-220-16-99

180000, г. Псков, пл. Ленина, д. 2

Laboratory of Integrated Environmental Research

21 Sovetskaya st., Pskov, 180000

taras_an@mail.ruМосковский государственный университет имени М.В. ЛомоносоваРоссияLomonosov Moscow State UniversityRussian FederationПсковский государственный университетРоссияPskov State UniversityRussian Federation202320112023783170177Copyright © Волгушева А.А., Конюхов И.В., Антал Т.К., 20232023Волгушева А.А., Конюхов И.В., Антал Т.К.Volgusheva А.А., Konyukhov I.V., Antal T.K.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestnik-bio-msu.elpub.ru/jour/article/view/1258

Регистрация кривых световой индукции флуоресценции хлорофилла высокого разрешения (т.н. OJIP-кривых) широко используется для оценки первичных реакций фотосинтеза фототрофных организмов. Современные флуоресцентные методы в совокупности с методами микроскопии предоставляют перспективную возможность регистрации OJIP-кривых на индивидуальных водорослевых клетках, что позволяет исследовать механизмы адаптации микроводорослей к стрессовым воздействиям, сопровождающиеся реорганизацией структуры популяции. В данной работе мы впервые охарактеризовали OJIP-кривые, полученные на индивидуальных водорослевых клетках с помощью оригинального микрофлуориметра, и сравнили их с кривыми, снятыми традиционно в суспензиях микроводорослей. По результатам исследования предложены метод анализа OJIP-кривых индивидуальных клеток микроводорослей, а также пути дальнейшего улучшения микрофлуориметрического метода.

High-resolution chlorophyll fluorescence light induction curves (OJIP transients) are widely used to assess the primary photosynthetic responses of phototrophic microorganisms. Chlorophyll fluorescence measuring methods coupled with microscopy techniques provide a promising opportunity to measure OJIP transients on individual algal cells, allowing scientists to investigate stress adaptation mechanisms related to reorganization of microalgae population or phytoplankton community. In this work, we first characterized the OJIP transients measured on individual algae cells using the original microfluorimeter and compared them with OJIP transients recorded in microalgae suspensions. Based on the results of the study, we proposed a method for analyzing OJIP curves of individual microalgae cells as well as ways to further improve microfluorimeters.

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The authors declare that there are no conflicts of interest present.