vestnik-bio-msuВестник Московского университета. Серия 16. БиологияVestnik Moskovskogo universiteta. Seriya 16. Biologiya0137-0952Lomonosov Moscow State University, School of Biology10.55959/MSU0137-0952-16-78-3S-7vestnik-bio-msu-1271Research ArticleКРАТКИЕ СООБЩЕНИЯSHORT COMMUNICATIONSСтруктура бычьего шаперонина TRiC/CCT в открытой конформации по данным криоэлектронной микроскопииCryo-EM structure of bovine chaperonin TRiC/CCT in open conformationhttps://orcid.org/0000-0002-8427-8178Станишнева-КоноваловаТ. Б.Stanishneva-KonovalovaT. B.

Станишнева-Коновалова Татьяна Борисовна – канд. биол. наук, ст. науч. сотр. кафедры биоинженерии биологического факультета

г. Москва, 119234, Ленинские горы, д. 1, стр. 12

Тел.: 8-495-939-41-95

Department of Bioengineering, Faculty of Biology

1–12 Leninskie Gory, 119234, Moscow

stanishneva-konovalova@mail.bio.msu.ruПичкурЕ. Б.PichkurE. B.

Пичкур Евгений Борисович – лаборант-исследователь Отдела структурной биологии

г. Москва, 123182, пл. Академика Курчатова, д. 1

Тел.: 8-499-196–95–39

Department of Structural Biology

1 Kurchatov Square, 123182, Moscow

eugene.pichkur@gmail.comКудрявцеваС. С.KudryavtsevaS. S.

Кудрявцева София Станиславовна – канд. биол. наук, мл. науч. сотр.

г. Москва, 119991, Ленинские горы, д. 1, стр. 40

Тел.: 8-495-939-53-59

1–40 Leninskie Gory, 119991, Moscow

sofiia.kudriavtceva@gmail.comЯрошевичИ. А.YaroshevichI. A.

Ярошевич Игорь Александрович – канд. биол. наук, науч. сотр. кафедры биофизики биологического факультета

г. Москва, 119234, Ленинские горы, д. 1, стр. 12

Тел.: 8-495-939-10-00

Department of Biophysics, Laboratory of Physical Chemistry of Biomembranes, Faculty of Biology

1–12 Leninskie Gory, 119234, Moscow

iyapromo@gmail.comСеменовА. Н.SemenovA. N.

Семенов Алексей Николаевич – канд. биол. наук, мл. науч. сотр. лаборатории физико-химии биомембран биологического факультета

г. Москва, 119234, Ленинские горы, д. 1, стр. 12

Тел.: 8-495-939-10-00

Laboratory of Physical Chemistry of Biomembranes, Faculty of Biology

1–12 Leninskie Gory, 119234, Moscow

semenov@physics.msu.ruМаксимовЕ. Г.MaksimovE. G.

Максимов Евгений Георгиевич – докт. биол. наук, зав. лабораторией физико-химии биомембран биологического факультета

г. Москва, 119234, Ленинские горы, д. 1, стр. 12

Тел.: 8-495-939-10-00

Laboratory of Physical Chemistry of Biomembranes, Faculty of Biology

1–12 Leninskie Gory, 119234, Moscow

emaksimoff@yandex.ruhttps://orcid.org/0000-0003-1112-2356МоисеенкоА. В.MoiseenkoA. V.

Моисеенко Андрей Владимирович – вед. инженер лаборатории электронной микроскопии биологического факультета

г. Москва, 119234, Ленинские горы, д. 1, стр. 32

Тел.: 8-495-939-33-59

Electron Microscopy Laboratory, Faculty of Biology

1–32 Leninskie Gory, 119234, Moscow

postmoiseenko@gmail.comВолохО. И.VolokhO. I.

Волох Олеся Игоревна – канд. биол. наук, науч. сотр. кафедры биоинженерии биологического факультета

г. Москва, 119234, Ленинские горы, д. 1, стр. 12

Тел.: 8-495-939-23-74

Department of Bioengineering, Faculty of Biology

1–12 Leninskie Gory, 119234, Moscow

olesyavolokh@icloud.comМуронецВ. И.MuronetzV. I.

Муронец Владимир Израилевич – док. биол. наук, проф., зав. отделом биохимии животной клетки

г. Москва, 119991, Ленинские горы, д. 1, стр. 40

Тел.: 8-495-939-53-59

Belozersky Institute of Physico-Chemical Biology

1–40 Leninskie Gory, 119991, Moscow

vimuronets@belozersky.msu.ruМосковский государственный университет имени М.В. ЛомоносоваРоссияLomonosov Moscow State UniversityRussian FederationНациональный исследовательский центр «Курчатовский институт»РоссияNational Research Centre “Kurchatov institute”Russian FederationНаучно-исследовательский институт физико-химической биологии имени А.Н. Белозерского, Московский государственный университет имени М.В. ЛомоносоваРоссияBelozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State UniversityRussian FederationНаучно-исследовательский институт физико-химической биологии имени А.Н. Белозерского, Московский государственный университет имени М.В. ЛомоносоваРоссияLomonosov Moscow State UniversityRussian Federation202301122023783S4046Copyright © Станишнева-Коновалова Т.Б., Пичкур Е.Б., Кудрявцева С.С., Ярошевич И.А., Семенов А.Н., Максимов Е.Г., Моисеенко А.В., Волох О.И., Муронец В.И., 20232023Станишнева-Коновалова Т.Б., Пичкур Е.Б., Кудрявцева С.С., Ярошевич И.А., Семенов А.Н., Максимов Е.Г., Моисеенко А.В., Волох О.И., Муронец В.И.Stanishneva-Konovalova T.B., Pichkur E.B., Kudryavtseva S.S., Yaroshevich I.A., Semenov A.N., Maksimov E.G., Moiseenko A.V., Volokh O.I., Muronetz V.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestnik-bio-msu.elpub.ru/jour/article/view/1271

В этой работе были подобраны условия для получения образца эукариотического шаперонина TRiC, пригодного для изучения методом криоэлектронной микроскопии. С помощью метода дифференциальной сканирующей (время-разрешенной) флуориметрии была охарактеризована температурная стабильность образцов белка при разных концентрациях соли и глицерина, а затем выбранные условия использовались для подготовки образца для микроскопии. В результате съемки и обработки изображений получена структура бычьего TRiC в открытой конформации с разрешением 4,42 Å.

In this work, conditions were selected for obtaining a sample of eukaryotic chaperonin TRiC suitable for studying by cryo-electron microscopy. Using the method of differential scanning (time-resolved) fluorimetry, the temperature stability of protein samples at different concentrations of salt and glycerol was compared, and then the selected conditions were used to prepare the sample for microscopy. As a result, the structure of bovine TRiC in an open conformation was obtained at 4.42 Å resolution.

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The authors declare that there are no conflicts of interest present.