vestnik-bio-msuВестник Московского университета. Серия 16. БиологияVestnik Moskovskogo universiteta. Seriya 16. Biologiya0137-0952Lomonosov Moscow State University, School of Biology10.55959/MSU0137-0952-16-78-3S-9vestnik-bio-msu-1273Research ArticleКРАТКИЕ СООБЩЕНИЯSHORT COMMUNICATIONSКриоэлектронная микроскопия на биологическом факультете МГУ имени М.В. ЛомоносоваCryo-electron microscopy at the Faculty of Biology of Lomonosov Moscow State Universityhttps://orcid.org/0000-0003-1112-2356МоисеенкоА. В.MoiseenkoA. V.

Моисеенко Андрей Владимирович – вед. инженер лаборатории электронной микроскопии биологического факультета

г. Москва, 119234, Ленинские горы, д. 1, стр. 32

Тел.: 8-495-939-33-59

Electron Microscopy Laboratory, Faculty of Biology

1–32 Leninskie Gory, 119234, Moscow

postmoiseenko@gmail.comЕгоровА. М.EgorovA. M.

Егоров Алексей Михайлович – докт. биол. наук, академик РАН, гл. науч. сотр. кафедры химической энзимологии химического факультета

г. Москва, 119234, Ленинские горы, д. 1, стр. 32

Тел.: 8-495-939-57-38

Department of Chemical Enzymology, Faculty of Chemistry

1–11 Leninskie Gory, 119234, Moscow

aegorov@enz.chem.msu.ruhttps://orcid.org/0000-0002-5137-303XШайтанК. В.ShaitanK. V.

Шайтан Константин Вольдемарович – докт. физ.-мат. наук, проф. кафедры биоинженерии биологического факультета

г. Москва, 119234, Ленинские горы, д. 1, стр. 73

Тел.: 8-495-939-57-38

Faculty of Biology

1–73 Leninskie Gory, 119234, Moscow

shaytan49@yandex.ruhttps://orcid.org/0000-0003-4678-232XСоколоваО. С.SokolovaO. S.

Соколова Ольга Сергеевна – докт. биол. наук, проф. кафедры биоинженерии биологического факультета

г. Москва, 119234, Ленинские горы, д. 1, стр. 73

Тел.: 8-495-939-57-38

Faculty of Biology

1–73 Leninskie Gory, 119234, Moscow

sokolova@mail.bio.msu.ruМосковский государственный университет имени М.В. ЛомоносоваРоссияLomonosov Moscow State UniversityRussian Federation202301122023783S5156Copyright © Моисеенко А.В., Егоров А.М., Шайтан К.В., Соколова О.С., 20232023Моисеенко А.В., Егоров А.М., Шайтан К.В., Соколова О.С.Moiseenko A.V., Egorov A.M., Shaitan K.V., Sokolova O.S.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestnik-bio-msu.elpub.ru/jour/article/view/1273

Продемонстрирован пример успешной модернизации аналитического просвечивающего электронного микроскопа JEOL JEM-2100 до криоэлектронного микроскопа низкого разрешения, предназначенного для решения задач отработки пробоподготовки и оценки качества препарата. В результате модернизации прибор позволяет получать субнанометровое разрешение реконструкций белковых молекул (в пределах 8 Å). Обсуждается влияние подложек из графена и аморфного углерода для предотвращения эффекта преимущественной ориентации белковых частиц в замороженном образце.

This paper demonstrates an example of a successful upgrade of a JEOL JEM-2100 analytical transmission electron microscope to a low-resolution cryo-electron microscope designed for routine tasks of sample preparation and quality evaluation. As a result of the upgrade, the instrument allows the subnanometer resolution of protein molecule reconstructions (within 8 Å). The influence of graphene and amorphous carbon support films to prevent the effect of preferred orientation of protein particles in the frozen sample is discussed.

криоэлектронная микроскопияструктурная биологияанализ единичных частицмодернизация оборудованияструктуравируснуклеосомаCryo-electron microscopystructural biologysingle particle analysisequipment upgradingstructurevirusnucleosomeМодернизация микроскопа проводилась в рамках проекта Министерства науки и высшего образования Российской Федерации. Анализ образцов и получение трехмерных структур вируса клещевого энцефалита выполнен при финансовой поддержке Программы развития МГУ (проект №23А-Ш04-01). Разработка методик из мерений нуклеосом производилась при поддержке Российского научного фонда (проект №19-74-30003). Работа проведена без использования животных и без привлечения людей в качестве испытуемых.The modernization of the microscope was carried out as part of a project of the Ministry of Science and Higher Education of the Russian Federation. 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The authors declare that there are no conflicts of interest present.