vestnik-bio-msuВестник Московского университета. Серия 16. БиологияVestnik Moskovskogo universiteta. Seriya 16. Biologiya0137-0952Lomonosov Moscow State University, School of Biology10.55959/MSU0137-0952-16-78-4-1vestnik-bio-msu-1282Research ArticleОРИГИНАЛЬНОЕ ИССЛЕДОВАНИЕRESEARCH ARTICLEДействие нейромедиаторов на программируемую клеточную смерть и образование активных форм кислорода в эпидермисе листьев горохаThe effect of neurotransmitters on programmed cell death and formation of reactive oxygen species in the epidermis of pea leaveshttps://orcid.org/0000-0001-6050-2940КиселевскийД. Б.KiselevskyD. B.

Киселевский Дмитрий Борисович – канд. биол. наук, ст. науч. сотр. кафедры иммунологии биологического факультета МГУ

dkiselevs@mail.ruhttps://orcid.org/0000-0002-6816-1615ОлескинА. В.OleskinA. V.

Олескин Александр Владимирович – докт. биол. наук, проф., проф. кафедры общей экологии и гидробиологии биологического факультета МГУ

aoleskin@rambler.ruСамуиловВ. Д.SamuilovV. D.

Самуилов Виталий Дмитриевич – докт. биол. наук, проф., гл. науч. сотр. кафедры иммунологии биологического факультета МГУ

vdsamuilov@mail.ruМосковский государственный университет имени М.В. ЛомоносоваРоссияLomonosov Moscow State UniversityRussian Federation202328122023784227–234Copyright © Киселевский Д.Б., Олескин А.В., Самуилов В.Д., 20232023Киселевский Д.Б., Олескин А.В., Самуилов В.Д.Kiselevsky D.B., Oleskin A.V., Samuilov V.D.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestnik-bio-msu.elpub.ru/jour/article/view/1282

Нейромедиаторы обнаружены не только у животных, но и у других живых организмов, включая растения. Однако данные о функциях этих соединений в растительном мире далеко не полны, в частности, не исследован вопрос об их влиянии на гибель растительных клеток. В настоящей работе испытано действие нейромедиаторов на программируемую гибель клеток и образование активных форм кислорода (АФК) у растений. Программируемую гибель клеток регистрировали по разрушению их ядер, а АФК определяли с помощью 2',7'-дихлорфлуоресцеина. Использовали дофамин, норадреналин, серотонин, гистамин, ацетилхолин и его синтетический аналог ацетилтиохолин. Катехоламины дофамин и норадреналин в концентрациях 0,01–1 мМ подавляли разрушение ядер устьичных клеток в эпидермисе листьев гороха, вызванное KCN. Серотонин и ацетилхолин в концентрации 1–3 мМ, напротив, усиливали KCN-индуцированное разрушение ядер. Гистамин и ацетилтиохолин не оказывали влияния на KCN-зависимое разрушение ядер в концентрациях 0,01–3 мМ. Ацетилтиохолин в концентрации 3 мМ, в отличие от природных нейромедиаторов, вызывал разрушение ядер устьичных клеток в отсутствие KCN. Дофамин, норадреналин и серотонин подавляли образование АФК в эпидермисе листьев гороха, вызванное менадионом. Гистамин, ацетилхолин и ацетилтиохолин не обладали подобным эффектом. Результаты показывают, что у растений дофамин, норадреналин и серотонин обладают свойствами антиоксидантов. Кроме того, дофамин и норадреналин могут защищать клетки от гибели.

Neurotransmitters are found not only in animals, but also in other living organisms, including plants. They are found in other living organisms, including plants. However, the data on the functions of these compounds in the plant world are far from being comprehensive. In particular, the issue concerning their impact on plant cell death still awaits further research.  In the present work, the effects of neurotransmitters on programmed cell death and the formation of reactive oxygen species (ROS) in plants were tested. Programmed cell death was estimated from the destruction of cell nuclei, and ROS was determined using 2',7'-dichlorofluorescein. Dopamine, norepinephrine, serotonin, histamine, acetylcholine and its synthetic analog acetylthiocholine were used. The catecholamines dopamine and norepinephrine at concentrations of 0.01–1 mM suppressed the destruction of guard cell nuclei in the epidermis of pea leaves, which was caused by KCN. Serotonin and acetylcholine at a concentration of 1–3 mM, on the contrary, increased the destruction of nuclei that was induced by KCN. Histamine and acetylthiocholine had no effect on KCN-dependent destruction of nuclei at concentrations of 0.01–3 mM. Acetylthiocholine at a concentration of 3 mM, in contrast to natural neurotransmitters, caused the destruction of guard cell nuclei in the absence of KCN. Dopamine, norepinephrine, and serotonin reduced the formation of ROS in the epidermis of pea leaves, which was induced by menadione. Histamine, acetylcholine and acetylthiocholine did not have a similar effect. The results demonstrate that dopamine, norepinephrine and serotonin have antioxidant properties in plants. In addition, dopamine and norepinephrine can prevent cell death.

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The authors declare that there are no conflicts of interest present.