vestnik-bio-msuВестник Московского университета. Серия 16. БиологияVestnik Moskovskogo universiteta. Seriya 16. Biologiya0137-0952Lomonosov Moscow State University, School of Biology10.55959/MSU0137-0952-16-79-2S-12vestnik-bio-msu-1385Research ArticleОРИГИНАЛЬНОЕ ИССЛЕДОВАНИЕRESEARCH ARTICLEРегуляция активности регенерирующих моторных синапсов с участием эндоканнабиноидовRegulation of neurotransmission in regenerating neuromuscular junctions involving endocannabinoidshttps://orcid.org/0000-0002-3525-4815БогачеваП. О.BogachevaP. O.

Богачева Полина Олеговна – канд. биол. наук, доц. кафедры физиологии человека и животных биологического факультета,

119234, г. Москва, Ленинские горы, д. 1, стр. 12

Department of Human and animal physiology, School of Biology, 

Leninskie gory 1–12, Moscow, 119234

untergang@inbox.ruhttps://orcid.org/0000-0002-7692-5080ЧернышевК. А.ChernyshevK. A.

Чернышев Кирилл Андреевич – студент магистратуры 2 года обучения кафедры физиологии человека и животных биологического факультета,

119234, г. Москва, Ленинские горы, д. 1, стр. 12

Department of Human and animal physiology, School of Biology, 

Leninskie gory 1–12, Moscow, 119234

cherkir2000@yandex.ruhttps://orcid.org/0000-0002-2233-1228ТарасоваЕ. О.TarasovaE. A.

Тарасова Екатерина Олеговна – канд. биол. наук, ст. науч. сотр. кафедры физиологии человека и животных биологического факультета,

119234, г. Москва, Ленинские горы, д. 1, стр. 12

Department of Human and animal physiology, School of Biology, 

Leninskie gory 1–12, Moscow, 119234

cate1990@mail.ruhttps://orcid.org/0009-0008-3866-647XПотаповаД. А.PotapovaD. A.

Потапова Диана Андреевна – студент магистратуры 1 года обучения кафедры физиологии человека и животных биологического факультета,

119234, г. Москва, Ленинские горы, д. 1, стр. 12

Department of Human and animal physiology, School of Biology, 

Leninskie gory 1–12, Moscow, 119234

potapovadiana2001@mail.ruhttps://orcid.org/0000-0002-6072-134XБалезинаО. П.BalezinaO. P.

Балезина Ольга Петровна – докт. биол. наук, проф. кафедры физиологии человека и животных биологического факультета,

119234, г. Москва, Ленинские горы, д. 1, стр. 12

Department of Human and animal physiology, School of Biology, 

Leninskie gory 1–12, Moscow, 119234

balezina@mail.ruМосковский государственный университет имени М.В. ЛомоносоваРоссияLomonosov Moscow State UniversityRussian Federation202427082024792S98104Copyright © Богачева П.О., Чернышев К.А., Тарасова Е.О., Потапова Д.А., Балезина О.П., 20242024Богачева П.О., Чернышев К.А., Тарасова Е.О., Потапова Д.А., Балезина О.П.Bogacheva P.O., Chernyshev K.A., Tarasova E.A., Potapova D.A., Balezina O.P.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestnik-bio-msu.elpub.ru/jour/article/view/1385

В работе исследовали влияние эндоканнабиноидов арахидоноилэтаноламида (АЭА), известного также как анандамид, и 2-арахидоноилглицерина (2-АГ) на параметры миниатюрных потенциалов концевой пластинки (МПКП) и вызванных потенциалов концевой пластинки (ПКП) моторных синапсов, восстанавливающихся в ходе реиннервации мышцы – длинного разгибателя пальцев мыши. 2-АГ увеличивал амплитуду МПКП на 35%, а также увеличивал амплитуду ПКП на 37%, не влияя при этом на квантовый состав ПКП и другие параметры секреции медиатора. Эффект предотвращался ингибитором везикулярного транспортера ацетилхолина везамиколом и обратным агонистом каннабиноидных рецепторов СВ1-типа АМ251. АЭА не вызывал изменений амплитуды и других параметров МПКП, однако снижал квантовый состав ПКП на 27%. Тормозный эффект АЭА предотвращался АМ251, а также блокатором Са2+-каналов L-типа нитрендипином. Впервые установлено, что в регенерирующих моторных синапсах АЭА и 2-АГ, активируя один и тот же тип пресинаптических СВ-рецепторов, имеют разные конечные мишени, влияют на разные параметры квантовой секреции ацетилхолина и оказывают разнонаправленное воздействие на синаптическую передачу. Наличие как облегчающих, так и тормозных эффектов эндоканнабиноидов может служить для тонкой настройки и регуляции синаптической передачи в созревающих моторных контактах.

The work was dedicated to investigation of the influence of two endocannabinoids – arachidonoylethanolamide (AEA), also known as anandamide, and 2-arachidonoylglycerol (2-AG) on the parameters of miniature endplate potentials (MEPP) and evoked endplate potentials (EPP) of motor synapses at the early stage of regeneration during muscle reinnervation. 2-AG increased the amplitude of MEPP by 35%, and also increased the amplitude of EPP by 37%, without affecting quantal content of EPP or any other parameters of neurotransmitter secretion. This effect was prevented by vesicular acetylcholine transporter inhibitor vesamicol and by inverse agonist of CB1-type cannabinoid receptors AM251. AEA did not change the amplitude or any other parameters of MEPP, but reduced the quantal content of EPP by 27%. The inhibitory effect of AEA was prevented by AM251 and by the L-type Ca2+ channel blocker nitrendipine. Thus, it was established for the first time that in newly formed motor synapses AEA and 2-AG activate the same type of presynaptic cannabinoid receptors, but have different final targets, influence different parameters of quantal ACh secretion and have multidirectional effects on synaptic transmission. The presence of both facilitatory and inhibitory effects of endocannabinoids in regenerating synapses may serve to fine-tune and regulate synaptic transmission during their maturation.

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The authors declare that there are no conflicts of interest present.