vestnik-bio-msuВестник Московского университета. Серия 16. БиологияVestnik Moskovskogo universiteta. Seriya 16. Biologiya0137-0952Lomonosov Moscow State University, School of Biology10.55959/MSU0137-0952-16-79-4-8vestnik-bio-msu-1436Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯRESEARCH ARTICLESВзаимодействующие гены PARG1 и EXA1 являются важными регуляторами аутоиммунитета у растенийPARG1 and EXA1 interacting genes are important regulators of autoimmunity in plantshttps://orcid.org/0000-0001-8106-5911КуприяноваЕ. В.KupriyanovaE. V.

Куприянова Евгения Владимировна – канд. биол. наук, ст. науч. сотр. кафедры 

119234, г. Москва, Ленинские горы, д. 1, стр. 12, Тел.: 8-495-939-54-90

Leninskie gory 1–12, Moscow, 119234 

ekupriyanova@gmail.comhttps://orcid.org/0000-0002-7405-1914МанаховаК. А.ManakhovaK. A.

Манахова Карина Айратовна – науч. сотр. (направление «Генетика») научного центра 

354340, Краснодарский край, пгт Сириус, Тел.: 8-800-100-41-55

Sirius, Sochi, Krasnodar region, 354340 

manahova.ka@talantiuspeh.ruhttps://orcid.org/0000-0002-5816-3051ЕжоваТ. А.EzhovaT. A.

Ежова Татьяна Анатольевна – докт. биол. наук, проф. кафедры 

119234, г. Москва, Ленинские горы, д. 1, стр. 12, Тел.: 8-495-939-54-90 

Leninskie gory 1–12, Moscow, 119234 

ezhova2001@gmail.comКафедра генетики, биологический факультет, Московский государственный университет имени М.В. ЛомоносоваРоссияDepartment of Genetics, Lomonosov Moscow State UniversityRussian FederationЦентр генетики и наук о жизни, Научно-технологический университет СириусРоссияCenter of Genetics and Life Science, Sirius University of Science and TechnologyRussian Federation202403032025794287297Copyright © Куприянова Е.В., Манахова К.А., Ежова Т.А., 20252025Куприянова Е.В., Манахова К.А., Ежова Т.А.Kupriyanova E.V., Manakhova K.A., Ezhova T.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestnik-bio-msu.elpub.ru/jour/article/view/1436

Исследования выполнены на растениях двойного мутанта резуховидки (Arabidopsis thaliana) parg1 exa1, характеризующегося смертью клеток по краю листовой пластинки, активацией экспрессии генов иммунных рецепторов, генов PR-белков и генов гиперчувствительного ответа, что является признаком аутоиммунитета. В то же время, в отличие от других мутантов c проявлением аутоиммунитета, мутант parg1 exa1 демонстрировал приобретение клетками листа плюрипотентности и способность к регенерации нового края листа. В связи с необычным фенотипом в данной работе проведен углубленный анализ транскриптома молодых листьев мутанта и растений дикого типа. У мутанта обнаружено повышение уровня экспрессии всех основных компонентов иммунного ответа, включая гены Са2+-проводящих каналов, НАДФН-оксидаз, генов синтеза салициловой кислоты, жасмоновой кислоты и многие другие. Полученные данные свидетельствуют о том, что гены PARG1 и EXA1, контролирующие уровень поли(АДФ-рибозилирования) и процесс нонсенс-опосредованной деградации РНК соответственно, являются важными регуляторами иммунного ответа. Их функция необходима для предотвращения чрезмерной активации защитных систем в растениях.

Studies were performed on the Arabidopsis double mutant plants parg1 exa1 characterized by cell death along the leaf margin, activation of expression of immune receptor genes, PR-genes and hypersensitive response genes, which is a sign of autoimmunity. At the same time, unlike other autoimmunity mutations, the parg1 exa1 mutant demonstrated the acquisition of pluripotency by leaf cells and the ability to regenerate new leaf margins. Because of the unusual phenotype, this study analyzed in depth the transcriptome of young leaves of mutant and wild-type plants. The mutant was found to have increased expression levels of all major components of the immune response, including genes for Ca2+ conducting channels, NADPH oxidases, salicylic acid synthesis genes, jasmonic acid, and many others. The findings suggest that PARG1 and EXA1 genes, which control the level of poly(ADP-ribosylation) and the process of nonsensemediated RNA degradation, respectively, are important regulators of the immune response. Their function is essential to prevent overactivation of defense systems in plants.

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The authors declare that there are no conflicts of interest present.