vestnik-bio-msuВестник Московского университета. Серия 16. БиологияVestnik Moskovskogo universiteta. Seriya 16. Biologiya0137-0952Lomonosov Moscow State University, School of Biology10.55959/MSU0137-0952-16-80-3-3vestnik-bio-msu-1560Research ArticleОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯRESEARCH ARTICLESСравнительное исследование слуховой чувствительности крыс линии Wistar и крыс-гетерозигот со сниженной экспрессией гена транспортера дофамина DAT-1Comparative study of auditory sensitivity in normal Wistar rats and heterozygous rats with reduced expression f the dopamine transporter (DAT-1) genehttps://orcid.org/0000-0002-2650-5619ХорунжийГ. Д.KhorunzhiiG. D.

Хорунжий Глеб Дмитриевич – канд. биол. наук, науч. сотр. группы нейрофизиологических основ механо-рецепторных функций

194223, г. Санкт-Петербург, пр. Тореза, д. 44

Тел.: 8-812-552-32-56

Torez Ave. 44, St. Petersburg, 194223

khorunzhii.gd@gmail.comhttps://orcid.org/0000-0002-4216-6469ЕгороваМ. А.EgorovaM. A.

Егорова Марина Александровна – канд. биол. наук, руководитель группы нейрофизиологических основ механо-рецепторных функций

194223, г. Санкт-Петербург, пр. Тореза, д. 44

Тел.: 8-812-552-32-56

Torez Ave. 44, St. Petersburg, 194223

ema6913@yandex.ruФесенкоЗ. С.FesenkoZ. S.

Фесенко Зоя Сергеевна – мл. науч. сотр. лаборатории нейробиологии и молекулярной фармакологи

199034, г. Санкт-Петербург, Университетская наб., д. 7/9

Тел.: 8-921-630-28-03

Universitetskaya Emb. 7/9, St. Petersburg, 199034

z.fesenko@spbu.ruhttps://orcid.org/0000-0002-9641-515XЕфимоваЕ. В.EfimovaE. V.

Ефимова Евгения Викторовна – канд. биол. наук, вед. науч. сотр. лаборатории нейробиологии и молекулярной фармакологи

199034, г. Санкт-Петербург, Университетская наб., д. 7/9

Тел.: 8-921-630-28-03

Universitetskaya Emb. 7/9, St. Petersburg, 199034

e.v.efimova@mail.ruИнститут эволюционной физиологии и биохимии имени И.М. Сеченова, Российская академия наукРоссияSechenov Institute of Evolutionary Physiology and Biochemistry, Russian Academy of SciencesRussian FederationСанкт-Петербургский государственный университет, Институт трансляционной биомедицины Санкт-Петербургского государственного университетаРоссияSaint Petersburg State University, Saint Petersburg State University Institute of Translational BiomedicineRussian Federation202526112025803165172Copyright © Хорунжий Г.Д., Егорова М.А., Фесенко З.С., Ефимова Е.В., 20252025Хорунжий Г.Д., Егорова М.А., Фесенко З.С., Ефимова Е.В.Khorunzhii G.D., Egorova M.A., Fesenko Z.S., Efimova E.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestnik-bio-msu.elpub.ru/jour/article/view/1560

Выполнено сравнительное исследование характеристик слуховой чувствительности интактных крыс линии Wistar и крыс-гетерозигот трансгенной линии DAT-het (dopamine transporter heterozygous) со сниженным уровнем экспрессии гена Slc6a3, кодирующего дофаминовый транспортер обратного захвата 1 (dopamine transporter 1, DAT-1), представляющих собой экспериментальную модель патологии системы дофаминергических нейронов (синдром дефицита внимания и гиперактивности). В ходе работы анализировали амплитудные и временные параметры коротколатентных стволовых слуховых вызванных потенциалов, зарегистрированных у крыс обеих линий при действии парных щелчков и одиночных тональных сигналов. Характеристики слуховой чувствительности крыс линий Wistar и DAT-het были сходными, но, тем не менее, сравнительный анализ амплитуд волн коротколатентных стволовых слуховых вызванных потенциалов выявил значимо бóльшую амплитуду волны 1 у крыс линии DAT-het. Полученные данные позволяют предположить, что гетерозиготы DAT-het отличаются от крыс с нормально функционирующим транспортером обратного захвата дофамина DAT-1 повышенным уровнем дофаминергической передачи сигнала, сопряженной с активацией D1-рецепторов, локализованных в мембранах нейронов спирального ганглия улитки внутреннего уха.

The comparative electrophysiological study of auditory sensitivity of intact Wistar rats and heterozygous rats of the transgenic DAT-het line with a reduced expression level of the Slc6a3 gene encoding the dopamine reuptake transporter (DAT-1), providing an experimental model of dopaminergic neurons pathology, was performed. The amplitude and time parameters of auditory brainstem responses recorded in rats of both lines under presentation of paired clicks and single tones were analyzed. Generally, the auditory sensitivities of Wistar and DAT-het rats were similar, but a comparative analysis of the amplitudes of auditory brainstem response peaks, obtained from experimental animals, revealed a significantly greater amplitude of peak 1 in DAT-het rats. Thus, the data obtained suggest that DAT-het heterozygotes differ from rats with a normally functioning dopamine reuptake DAT-1 transporter by an increased level of dopaminergic signaling via activation of D1 receptors localized in the membranes of neurons of the cochlea spiral ganglion.

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The authors declare that there are no conflicts of interest present.