vestnik-bio-msuВестник Московского университета. Серия 16. БиологияVestnik Moskovskogo universiteta. Seriya 16. Biologiya0137-0952Lomonosov Moscow State University, School of Biologyvestnik-bio-msu-259Research ArticleГеронтологияGerontologyК ВОПРОСУ О СНЯТИИ КРИВЫХ ВЫЖИВАНИЯ КУЛЬТИВИРУЕМЫХ КЛЕТОК В ЦИТОГЕРОНТОЛОГИЧЕСКИХ ЭКСПЕРИМЕНТАХ: КОРОТКИЙ КОММЕНТАРИЙ С ТРЕМЯ ИЕРАРХИЧЕСКИМИ ДИАГРАММАМИON THE CONSTRUCTING OF SURVIVAL CURVES FOR CULTURED CELLS IN CYTOGERONTOLOGICAL EXPERIMENTS: A BRIEF NOTE WITH THREE HIERARCHY DIAGRAMSХохловАлександр НиколаевичKhokhlovA. N.

докт. биол. наук, зав. сектором эволюционной цитогеронтологии

khokhlov@mail.bio.msu.ruМоргуноваГалина ВасильевнаMorgunovaG. V.

аспирант сектора эволюционной цитогеронтологии

morgunova@mail.bio.msu.ruбиологический факультет МГУRussian Federation201517082015021923Copyright © Хохлов А.Н., Моргунова Г.В., 20152015Хохлов А.Н., Моргунова Г.В.Khokhlov A.N., Morgunova G.V.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestnik-bio-msu.elpub.ru/jour/article/view/259

Проведен краткий сравнительный анализ различных подходов к оценке жизнеспособ­ ности клеток в цитогеронтологических экспериментах. Особый акцент сделан на проблемах, возникающих при снятии кривых выживания культивируемых клеток в модели “стационар­ного старения”. Подчеркивается, что выбор соответствующих методов определяется главным образом представлениями исследователей о молекулярно­клеточных механизмах старения. Отмечается, что хотя оценка эффективности образования клетками колоний является опти­мальным подходом к определению их жизнеспособности, она, к сожалению, неприменима для постмитотических или очень медленно размножающихся клеток. Рассматриваются также некоторые проблемы, возникающие при использовании наиболее популярных молекулярных зондов, предназначенных для оценки соотношения живых и мертвых клеток в клеточной популяции.

A brief comparative analysis of different approaches to cell viability testing in cytogerontological experiments is made. Particular emphasis is placed upon problems arising under constructing of survival curves for cultured cells in the “stationary phase aging” model. It is noted that the choice of the appropriate methods is mainly determined by the researchers‘ ideas about molecular and cellular mechanisms of aging. It is emphasized that the evaluation of cell cloning efficiency, though optimal for cell viability determination, is, unfortunately, non­applicable to postmitotic or very slowly propagating cells. Some problems encountered when using the most popular molecular probes designed for “live/dead” assays of cell population are also briefly reviewed.

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The authors declare that there are no conflicts of interest present.