vestnik-bio-msuВестник Московского университета. Серия 16. БиологияVestnik Moskovskogo universiteta. Seriya 16. Biologiya0137-0952Lomonosov Moscow State University, School of Biologyvestnik-bio-msu-293Research ArticleБиохимияBiochemistryСАЛИЦИЛГИДРОКСАМАТ УСИЛИВАЕТ НАДН-ОКСИДАЗНУЮ АКТИВНОСТЬ ПЕРОКСИДАЗЫ В СУСПЕНЗИЯХ МИТОХОНДРИЙ И ХЛОРОПЛАСТОВ ГОРОХАSALICYLHYDROXAMIC ACID ACCELERATES THE NADH OXIDASE ACTIVITY OF PEROXIDASE IN SUSPENSIONS OF PEA MITOCHONDRIA AND CHLOROPLASTSСамуиловВ. Д.SamuilovV. D.

докт. биол. наук, гл. науч. сотр. кафедры иммунологии биологического факультета МГУ. Тел.: 8-495-939-13-56

vdsamuilov@mail.ruКиселевскийД. Б.KiselevskyD. B.

канд. биол. наук, ст. науч. сотр. кафедры иммунологии биологического факультета МГУ. Тел.: 8-495-939-13-56

dkiselevs@mail.ruКафедра иммунологии, биологический факультет, Московский государственный университет имени М.В. Ломоносова; Россия, 119234, г. Москва, Ленинские горы, д. 1, стр. 12РоссияDepartment of Immunology, School of Biology, M.V. Lomonosov Moscow State University, Leninskiye gory 1-12, Moscow, 119234, RussiaRussian FederationКафедра иммунологии, биологический факультет, Московский государственный университет имени М.В. Ломоносова; Россия, 119234, г. Москва, Ленинские горы, д. 1, стр. 12РоссияDepartment of Immunology, School of Biology, M.V. Lomonosov Moscow State University, Leninskiye gory 1-12, Moscow, 119234, RussiaRussian Federation201614032016012328Copyright © Самуилов В.Д., Киселевский Д.Б., 20162016Самуилов В.Д., Киселевский Д.Б.Samuilov V.D., Kiselevsky D.B.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestnik-bio-msu.elpub.ru/jour/article/view/293

Салицилгидроксамат (СГ), ингибитор альтернативной оксидазы митохондрий растений, усиливает НАДH-оксидазную активность в суспензиях митохондрий и хлоропластов, полученных при их выделении из корней или листьев гороха, соответственно. Реакция подавляется при отмывании митохондрий и хлоропластов и проявляется в надосадочных растворах, полученных при их удалении центрифугированием. Реакция чувствительна к CN– и антиоксиданту пропилгаллату. Окисление НАДН, наряду с СГ, стимулируется 2,4-дихлорфенолом или фенолом, но не салициловой кислотой. Ускорение окисления НАДН фенольными соединениями наблюдается с коммерческой пероксидазой хрена, оно связано с участием этих соединений в НАДH-зависимой пероксидазной реакции. 2,4-Дихлорфенол и СГ значительно усиливают разрушение ядер устьичных клеток в эпидермисе из листьев гороха, вызванное образованием активных форм кислорода при окислении добавленного НАДН с участием апопластной пероксидазы.

Salicylhydroxamic acid (SHAM), an inhibitor of the alternative oxidase in plant mitochondria, accelerated the NADH-oxidase activity in suspensions of mitochondria and chloroplasts, obtained by their isolation from the roots or leaves of pea, respectively. The reaction was suppressed by washing mitochondria and chloroplasts. It also proceeded in supernatants where the organelles were removed by centrifugation. The reaction was sensitive to CN– and propyl gallate, an antioxidant. In addition to SHAM, NADH oxidation was stimulated by 2,4-dichlorophenol or phenol, but not by salicylic acid. The acceleration of NADH oxidation by the phenolic compounds occurred in the presence of commercial horseradish peroxidase. It is due to the involvement of these compounds in the NADH-dependent peroxidase reaction. 2,4-Dichlorophenol and SHAM enhanced significantly destruction of nuclei in guard cells of the epidermis from pea leaves induced by generation of reactive oxygen species under oxidation of exogenous NADH by means of the apoplastic peroxidase.

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The authors declare that there are no conflicts of interest present.