vestnik-bio-msuВестник Московского университета. Серия 16. БиологияVestnik Moskovskogo universiteta. Seriya 16. Biologiya0137-0952Lomonosov Moscow State University, School of Biologyvestnik-bio-msu-313Research ArticleФИЗИОЛОГИЯ МИКРООРГАНИЗМОВMICROBIOL PHYSIOLOGYВлияние освещенности и азотного голодания на рост и накопление арахидоновой кислоты у одноклеточной водоросли Parietochloris incisaEffects of light irradiance and nitrogen starvation on the accumulation of arachidonic acid by the microalga Parietochloris incisaСоловченкоА. Е.SolovchenkoA. E.

кафедра физиологии микроорганизмов

Department of Microorganisms Physiology

МерзлякМ. Н.MerzlyakM. N.

кафедра физиологии микроорганизмов

Department of Microorganisms Physiology

m_merzlyak@mail.ruЧ.О. Б.ChivkunovaO. B.

кафедра физиологии микроорганизмов

Department of Microorganisms Physiology

РешетниковаИ. В.ReshetnikovaI. V.

кафедра физиологии микроорганизмов

Department of Microorganisms Physiology

Хозина-ГолдбергИ.Khozin-GoldbergI.

Лаборатория биотехнологии микроводорослей, Институт изучения пустынь им. Дж. Блауштейна

Laboratory of Biotechnology of Microalgae, J. Blaustein Institute for the Study of Deserts

khozin@bgu.ac.ilДиди-КоэнШ.Didi-CohenS.

Лаборатория биотехнологии микроводорослей, Институт изучения пустынь им. Дж. Блауштейна

Laboratory of Biotechnology of Microalgae, J. Blaustein Institute for the Study of Deserts

КоэнЦ.CohenZ.

Лаборатория биотехнологии микроводорослей, Институт изучения пустынь им. Дж. Блауштейна

Laboratory of Biotechnology of Microalgae, J. Blaustein Institute for the Study of Deserts

Московский государственный университет имени М.В.ЛомоносоваРоссияLomosov Moscow State UniversityRussian FederationУниверситет Бен-ГурионаИзраильBen-Gurion UniversityIsrael200801042008014953Copyright © Соловченко А.Е., Мерзляк М.Н., Ч. О.Б., Решетникова И.В., Хозина-Голдберг И., Диди-Коэн Ш., Коэн Ц., 20082008Соловченко А.Е., Мерзляк М.Н., Ч. О.Б., Решетникова И.В., Хозина-Голдберг И., Диди-Коэн Ш., Коэн Ц.Solovchenko A.E., Merzlyak M.N., Chivkunova O.B., Reshetnikova I.V., Khozin-Goldberg I., Didi-Cohen S., Cohen Z.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestnik-bio-msu.elpub.ru/jour/article/view/313

Материалы международной конференции “Физиология микроорганизмов в природных и экспериментальных системах”, посвященной памяти проф. М.В. Гусева. МГУ. Биологический факультет. 16—19 мая 2006 г.

Parietochloris incisa is a unicellular, fresh water green alga, capable of accumulating high amounts of the valuable very long chain polyunsaturated arachidonic acid (AA) in triacylglycerols (TAG) of cytoplasmic oil-bodies. To find out the cultivation conditions providing maximum AA yield, the effects of light irradiance and N- availability on the dry weight (DW), chlorophyll, carotenoid and AA content have been studied. Under nitrogen starvation, TAG accounted for over 30% of dry weight (DW) and AA content became as high as ~ 55% of total fatty acids. From the standpoint of biomass accumulation, light intensity of ca. 400 uE m–2 · s–1 was found to be optimal for growing P. incisa on complete medium. Lower light intensities (or higher cell density of inoculum) resulted in higher AA yield when the alga was cultivated on nitrogen-free media. In the absence of nitrogen, algal cells were unable to cope with high light and suffered from photooxidative damage, whereas the nitrogen-sufficient culture survived under such illumination conditions probably due to accumulation of carotenoids. Nitrogen-deprived P. incisa cells displayed elevated sensitivity to light.

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The authors declare that there are no conflicts of interest present.