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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vestnik-bio-msu</journal-id><journal-title-group><journal-title xml:lang="ru">Вестник Московского университета. Серия 16. Биология</journal-title><trans-title-group xml:lang="en"><trans-title>Vestnik Moskovskogo universiteta. Seriya 16. Biologiya</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">0137-0952</issn><publisher><publisher-name>Lomonosov Moscow State University,  School of Biology</publisher-name></publisher></journal-meta><article-meta><article-id custom-type="elpub" pub-id-type="custom">vestnik-bio-msu-342</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>Методы</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>Methods</subject></subj-group></article-categories><title-group><article-title>ИССЛЕДОВАНИЕ ЖИЗНЕСПОСОБНОСТИ КУЛЬТИВИРУЕМЫХ КЛЕТОК ЧЕЛОВЕКА В СУСПЕНЗИИ</article-title><trans-title-group xml:lang="en"><trans-title>INVESTIGATION OF HUMAN CULTURED CELLS VIABILITY IN SUSPENSION</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Роговая</surname><given-names>О. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Rogovaya</surname><given-names>O. S.</given-names></name></name-alternatives><bio xml:lang="ru"><p>канд. биол. наук, мл. науч. сотр. лаборатории клеточной биологии ИБР РАН. Тел.: 8-499-135-40-81</p></bio><email xlink:type="simple">rogovaya26f@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Петракова</surname><given-names>О. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Petrakova</surname><given-names>O. S.</given-names></name></name-alternatives><bio xml:lang="ru"><p>канд. биол. наук, науч. сотр. кафедры эмбриологии биологического факультета МГУ. Тел.: 8-495-939-14-62</p></bio><email xlink:type="simple">petrakovaol@yandex.ru</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Гвазава</surname><given-names>И. Г.</given-names></name><name name-style="western" xml:lang="en"><surname>Gvazava</surname><given-names>I. G.</given-names></name></name-alternatives><bio xml:lang="ru"><p>канд. биол. наук, науч. сотр. лаборатории клеточной биологии ИБР РАН. Тел.: 8-499-135-40-81</p></bio><email xlink:type="simple">gvazava.inessa@yandex.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Борисов</surname><given-names>М. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Borisov</surname><given-names>M. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>аспирант лаборатории клеточной биологии ИБР РАН. Тел.: 8-499-135-40-81</p></bio><email xlink:type="simple">borisov.mikhail2011@yandex.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Васильев</surname><given-names>А. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Vasiliev</surname><given-names>A. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>директор ИБР РАН. Тел.: 8-499-135-33-22</p></bio><email xlink:type="simple">113162@bk.ru</email><xref ref-type="aff" rid="aff-3"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Институт биологии развития им. Н.К. Кольцова РАН; Российский национальный исследовательский медицинский университет им. Н.И. Пирогова Минздрава России</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Koltsov Institute of Developmental Biology, Russian Academy of Sciences; Pirogov Russian National Research Medical University</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>Российский национальный исследовательский медицинский университет им. Н.И. Пирогова Минздрава России; Московский государственный университет им. М.В. Ломоносова</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Pirogov Russian National Research Medical University; Lomonosov Moscow State University</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-3"><aff xml:lang="ru"><institution>Институт биологии развития им. Н.К. Кольцова РАН; Московский государственный университет им. М.В. Ломоносова</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Koltsov Institute of Developmental Biology, Russian Academy of Sciences; Lomonosov Moscow State University</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2016</year></pub-date><pub-date pub-type="epub"><day>31</day><month>07</month><year>2016</year></pub-date><volume>0</volume><issue>3</issue><fpage>44</fpage><lpage>48</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Роговая О.С., Петракова О.С., Гвазава И.Г., Борисов М.А., Васильев А.В., 2016</copyright-statement><copyright-year>2016</copyright-year><copyright-holder xml:lang="ru">Роговая О.С., Петракова О.С., Гвазава И.Г., Борисов М.А., Васильев А.В.</copyright-holder><copyright-holder xml:lang="en">Rogovaya O.S., Petrakova O.S., Gvazava I.G., Borisov M.A., Vasiliev A.V.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://vestnik-bio-msu.elpub.ru/jour/article/view/342">https://vestnik-bio-msu.elpub.ru/jour/article/view/342</self-uri><abstract><p>Успех клеточной терапии напрямую связан с жизнеспособностью трансплантируемых клеток. В ряде случаев клетки вводят в суспензии. Однако на данный момент не подобраны оптимальные условия для сохранения жизнеспособности клеток при приготовлении суспензионного клеточного трансплантата и при его хранении. Цель работы заключалась в поиске оптимальных условий для хранения суспензии клеток поднижнечелюстной слюнной железы, дифференцированных клеток поднижнечелюстной слюнной железы и дермальных фибробластов человека в физиологически совместимых растворах. В работе использовали стандартные методы выделения и культивирования клеток. Подсчет количества клеток осуществляли на автоматическом счетчике клеток BioRad, жизнеспособность клеток оценивали окрашиванием 4%-ным трипановым синим. В качестве биологически совместимых растворов тестировали фосфатно-солевой буфер, физиологический раствор для инъекций и 2%-ный раствор альбумина человека в фосфатно-солевом буфере. В результате работы было выявлено, что тестируемые клетки человека сохраняют жизнеспособность в суспензии во всех исследуемых растворах при +4°С и +25°С, как минимум в течение 24 ч. Наибольшая жизнеспособность клеток слюнной железы (более 50%) наблюдается в фосфатно-солевом буфере при обоих исследованных температурных режимах. Однако при +4°С клетки слюнной железы лучше сохраняют адгезивные и пролиферативные свойства после 24 ч инкубации в данных условиях. При тестировании фибробластов показано, что в физиологическом растворе клетки сохраняются в виде равномерной одноклеточной суспензии и практически не теряют жизнеспособности в течение 30 ч при +4°С. Добавление 2% альбумина снижает жизнеспособность фибробластов. Таким образом, на основании проведенных исследований рекомендовано хранить и транспортировать суспензию клеток поднижнечелюстной слюнной железы человека в фосфатно-солевом буфере при +4°С; фибробласты человека — в физиологическом растворе при +4°С.</p></abstract><trans-abstract xml:lang="en"><p>The success of cellular therapy is directly related to the viability of transplanted cells. In some cases the cells may be injected as suspension. However, the optimal conditions for maintain of the cell viability during graft preparation and cell suspension storage have not yet been established. Purpose of this study is to investigate the optimal conditions for storage of suspensions of human submandibular salivary gland cells, differentiated submandibular salivary gland cells and dermal fibroblasts in a physiologically compatible solutions. Standard methods for cell isolation and cultivation were used. Counting was performed on an automatic cell counter BioRad, cell viability was assessed by staining with 4% Trypan blue. As biocompatible solutions we tested phosphate-buffered saline, saline solution for injection and a 2% solution of human albumin in phosphate-buffered saline. It was found that the tested human cells retain viability in suspension in all solutions for at least 24 hours at +4°C and +25°C. Highest salivary gland cells viability (more 50%) was observed in phosphate buffered saline at both storage temperatures. However, the salivary gland cells better maintain adhesive and proliferative properties after 24 hours of incubation at +4°C. The study of fibroblasts shows that in saline solution these cells are preserved as a single cell suspension and hardly lose viability during 30 hours storage at +4°C. Addition of 2% albumin reduces the viability of fibroblasts. Based on our studies we recommend to store and transport the human submandibular salivary gland cells in phosphate buffered saline at +4°C; human fibroblasts — in saline solution at +4°C.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>жизнеспособность</kwd><kwd>клетки поднижнечелюстной слюнной железы человека</kwd><kwd>суспензия</kwd><kwd>фибробласты человека</kwd><kwd>хранение суспензионных трансплантатов</kwd><kwd>культуры клеток человека</kwd></kwd-group><kwd-group xml:lang="en"><kwd>cell viability</kwd><kwd>human submandibular salivary gland cells</kwd><kwd>suspension</kwd><kwd>human fibroblasts</kwd><kwd>suspension grafts storage</kwd><kwd>human cell cultures</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">FDA. 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