<?xml version="1.0" encoding="UTF-8"?>
<!DOCTYPE article PUBLIC "-//NLM//DTD JATS (Z39.96) Journal Publishing DTD v1.3 20210610//EN" "JATS-journalpublishing1-3.dtd">
<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vestnik-bio-msu</journal-id><journal-title-group><journal-title xml:lang="ru">Вестник Московского университета. Серия 16. Биология</journal-title><trans-title-group xml:lang="en"><trans-title>Vestnik Moskovskogo universiteta. Seriya 16. Biologiya</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">0137-0952</issn><publisher><publisher-name>Lomonosov Moscow State University,  School of Biology</publisher-name></publisher></journal-meta><article-meta><article-id custom-type="elpub" pub-id-type="custom">vestnik-bio-msu-476</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>Физиология растений</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>Plant Physiology</subject></subj-group></article-categories><title-group><article-title>ВЛИЯНИЕ УЛЬТРАФИОЛЕТОВОГО ИЗЛУЧЕНИЯ СРЕДНЕВОЛНОВОГО ДИАПАЗОНА НА СОДЕРЖАНИЕ И СПЕКТР ПОЛИАМИНОВ В ЛИСТЬЯХ И КОРНЯХ ДИКОРАСТУЩИХ РАСТЕНИЙ</article-title><trans-title-group xml:lang="en"><trans-title>THE EFFECT OF MEDIUM WAVE ULTRAVIOLET RADIATION ON THE CONTENT AND SPECTRUM OF POLYAMINES IN LEAVES AND ROOTS OF WILD-GROWING PLANTS</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Радюкина</surname><given-names>Н. Л.</given-names></name><name name-style="western" xml:lang="en"><surname>Radyukina</surname><given-names>N. L.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Россия, 127276, г. Москва, ул. Ботаническая, д. 35</p><p>докт. биол. наук, ст. науч. сотр. Института физиологии растений имени К.А. Тимирязева РАН. Тел. 8-499-231-83-59</p></bio><bio xml:lang="en"><p>Botanicheskaya ul. 354, 127276, Moscow, Russia</p></bio><email xlink:type="simple">natrad@yandex.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Иванов</surname><given-names>Ю. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Ivanov</surname><given-names>Y. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Россия, 127276, г. Москва, ул. Ботаническая, д. 35</p><p>канд. биол. наук, ст. науч. сотр. Института физиологии растений имени К.А.Тимирязева РАН. Тел. 8-499-231-83-59</p></bio><bio xml:lang="en"><p>Botanicheskaya ul. 354, 127276, Moscow, Russia</p></bio><email xlink:type="simple">ivanovinfo@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Мапелли</surname><given-names>С.</given-names></name><name name-style="western" xml:lang="en"><surname>Mapelli</surname><given-names>S.</given-names></name></name-alternatives><bio xml:lang="ru"><p>National Research Council</p><p>via Bassini 15, 20133 Milano, Italy</p><p>науч. сотр. Института сельскохозяйственной биологии и биотехнологии Национального совета по научным исследованиям, Милан, Италия. Тел.: +39 0223699 430</p></bio><bio xml:lang="en"><p>National Research Council</p><p>via Bassini 15, 20133 Milano, Italy</p></bio><email xlink:type="simple">mapelli@ibba.cnr.it</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Михеева</surname><given-names>Л. Е.</given-names></name><name name-style="western" xml:lang="en"><surname>Mikheeva</surname><given-names>L. E.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Кафедра генетики, биологический факультет</p><p>Россия, 119234, г. Москва, Ленинские горы, д.1, стр. 12</p><p>канд. биол. наук, вед. науч. сотр. кафедры генетики биологического факультета МГУ. Тел. 8-495-939-11-79</p></bio><bio xml:lang="en"><p>Department of Genetics, School of Biology</p><p>Leninskiye gory 1–12, Moscow, 119234, Russia</p></bio><email xlink:type="simple">lidamikheeva@yandex.ru</email><xref ref-type="aff" rid="aff-3"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Карбышева</surname><given-names>Е. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Karbysheva</surname><given-names>E. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>Кафедра генетики, биологический факультет</p><p>Россия, 119234, г. Москва, Ленинские горы, д.1, стр. 12</p><p>канд. биол. наук, вед. науч. сотр. кафедры генетики биологического факультета МГУ. Тел. 8-495-939-11-79</p></bio><bio xml:lang="en"><p>Department of Genetics, School of Biology</p><p>Leninskiye gory 1–12, Moscow, 119234, Russia</p></bio><email xlink:type="simple">karbisheva@mail.ru</email><xref ref-type="aff" rid="aff-3"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Институт физиологии растений имени К.А. Тимирязева, РАН</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Timiryazev Institute of Plant Physiology, Russian Academy of Sciences</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>Institute of Agricultural Biology and Biotechnology</institution><country>Италия</country></aff><aff xml:lang="en"><institution>Institute of Agricultural Biology and Biotechnology</institution><country>Italy</country></aff></aff-alternatives><aff-alternatives id="aff-3"><aff xml:lang="ru"><institution>Московский государственный университет имени М.В. Ломоносова</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Lomonosov Moscow State University</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2017</year></pub-date><pub-date pub-type="epub"><day>28</day><month>07</month><year>2017</year></pub-date><volume>72</volume><issue>3</issue><fpage>179</fpage><lpage>183</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Радюкина Н.Л., Иванов Ю.В., Мапелли С., Михеева Л.Е., Карбышева Е.А., 2017</copyright-statement><copyright-year>2017</copyright-year><copyright-holder xml:lang="ru">Радюкина Н.Л., Иванов Ю.В., Мапелли С., Михеева Л.Е., Карбышева Е.А.</copyright-holder><copyright-holder xml:lang="en">Radyukina N.L., Ivanov Y.V., Mapelli S., Mikheeva L.E., Karbysheva E.A.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://vestnik-bio-msu.elpub.ru/jour/article/view/476">https://vestnik-bio-msu.elpub.ru/jour/article/view/476</self-uri><abstract><p>Настоящая работа является продолжением серии работ по изучению кратковременного действия ультрафиолетового излучения средневолнового диапазона (12,5 кДж/м2) на растения. При этом особое внимание уделено быстрому ответу антиоксидантной системы (измерение через 24 ч после облучения). Свободные и конъюгированные формы полиаминов (ПА) путресцинового ряда (путресцин, спермин, спермидин) и кадаверина, как известно, являются частью антиоксидантной системы. В растениях Thellungiella salsuginea, Salvia officinalis L., Plantago major L., Geum urbanum L., выращенных в водной культуре в условиях фитотрона определяли содержание свободных и конъюгированных форм ПА через 24 ч после действия ультрафиолетового излучения средневолнового диапазона. Полученные данные подтверждают существующую гипотезу о наибольшей роли путресцина в защитном ответе растений на действие ультрафиолетового излучения средневолнового диапазона. Повышение содержания этого ПА наблюдалось в листьях трёх из четырёх исследованных растений, и именно изменения в его содержании определяли суммарное повышение уровня свободных ПА. В уровне конъюгированных форм спермина, спермидина и кадаверина не удалось выявить общую тенденцию, и только для конъюгатов путресцина отмечено чёткое повышение. Проведенные исследования позволяют сделать вывод о том, что относительный вклад конкретных форм ПА в защитный ответ определяется прежде всего видовой принадлежностью исследованных растений. Вполне вероятно, что конъюгированные формы ПА могут служить резервом для быстрого восстановления необходимого уровня эндогенных свободных форм ПА.</p></abstract><trans-abstract xml:lang="en"><p>This work is a continuation of a series of studies on the short-term effects of UV-B radiation (12.5 kJ/m2) on plants with particular attention paid to the rapid response of the antioxidant system (measurement 24 hrs after irradiation). Free and conjugated forms of putrescine polyamines (putrescine, spermine, spermidine) and cadaverine are known to be part of the antioxidant system. In the plants Thellungiella salsuginea, Salvia officinalis L, Plantago major L., and Geum urbanum L. grown in aquatic culture under phytotron conditions, the content of free and conjugated forms of polyamins was determined 24 hrs after the treatment with UV-B. The obtained data confirm the existing hypothesis about the major role of putrescine in the protective response of plants to UV-B irradiation. An increase in the content of this polyamine was observed in the leaves of three of the four plants studied. And it was the changes in its content that determined the total increase in the level of free polyamines. It was not possible to reveal a general trend in the level of conjugated forms of spermine, spermidine and cadaverine, and only for putrescine conjugates there was a clear increase. The data obtained allow to conclude that the relative contribution of specific forms of polyamines to the protective response is determined primarily by the species of the investigated plants. It is likely that conjugated forms of polyamines can serve as a reserve for rapid recovery of the required level of endogenous free forms of polyamines.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>дикорастущие растения</kwd><kwd>свободные полиамины</kwd><kwd>конъюгированные полиамины</kwd><kwd>излучение УФ-B</kwd><kwd>окислительный стресс</kwd><kwd>антиоксидантная система</kwd></kwd-group><kwd-group xml:lang="en"><kwd>wild-growing plants</kwd><kwd>free polyamines</kwd><kwd>conjugated polyamines</kwd><kwd>UV-B irradiation</kwd><kwd>oxidative stress</kwd><kwd>antioxidant system</kwd></kwd-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Miura K., Tada Y. Regulation of water, salinity and cold stress responses by salicylic acid // Front. Plant Sci. 2014. Vol. 5. Article 4, doi: https://doi.org/10.3389/fpls.2014.00004.</mixed-citation><mixed-citation xml:lang="en">Miura K., Tada Y. Regulation of water, salinity and cold stress responses by salicylic acid // Front. Plant Sci. 2014. Vol. 5. Article 4, doi: https://doi.org/10.3389/fpls.2014.00004.</mixed-citation></citation-alternatives></ref><ref id="cit2"><label>2</label><citation-alternatives><mixed-citation xml:lang="ru">Kuznetsov V.V., Shevyakova N.I. Polyamines and plant adaptation to saline environment // Desert Plants. Biology and Biotechnology / Ed. K.B. Ramawat. Berlin-Heidelberg: Spinger, 2010. P. 261–297.</mixed-citation><mixed-citation xml:lang="en">Kuznetsov V.V., Shevyakova N.I. Polyamines and plant adaptation to saline environment // Desert Plants. Biology and Biotechnology / Ed. K.B. Ramawat. Berlin-Heidelberg: Spinger, 2010. P. 261–297.</mixed-citation></citation-alternatives></ref><ref id="cit3"><label>3</label><citation-alternatives><mixed-citation xml:lang="ru">Bouchereau A., Aziz A., Larher F., Martin-Tanguy J. Polyamines and environmental challenges: recent development // Plant Sci. 1999. Vol. 140. N 2. P. 103–125.</mixed-citation><mixed-citation xml:lang="en">Bouchereau A., Aziz A., Larher F., Martin-Tanguy J. Polyamines and environmental challenges: recent development // Plant Sci. 1999. Vol. 140. N 2. P. 103–125.</mixed-citation></citation-alternatives></ref><ref id="cit4"><label>4</label><citation-alternatives><mixed-citation xml:lang="ru">Kaur-Sawhney R., Tiburcio A.F., Altabella T., Galston A.W. Polyamines in plants: An overview // J. Cell Mol. Biol. 2003. Vol. 2. N 1. P. 1–12.</mixed-citation><mixed-citation xml:lang="en">Kaur-Sawhney R., Tiburcio A.F., Altabella T., Galston A.W. Polyamines in plants: An overview // J. Cell Mol. Biol. 2003. Vol. 2. N 1. P. 1–12.</mixed-citation></citation-alternatives></ref><ref id="cit5"><label>5</label><citation-alternatives><mixed-citation xml:lang="ru">Korolkova D.V., Radyukina N.L., Soshinkova T.N., Mapelli S., Kuznetsov V.V. Effect of spermine treatment on the functioning of Thellungiella salsuginea // Russ. J. Plant Physiol. 2014. Vol. 61. N 1. P. 69–76.</mixed-citation><mixed-citation xml:lang="en">Korolkova D.V., Radyukina N.L., Soshinkova T.N., Mapelli S., Kuznetsov V.V. Effect of spermine treatment on the functioning of Thellungiella salsuginea // Russ. J. Plant Physiol. 2014. Vol. 61. N 1. P. 69–76.</mixed-citation></citation-alternatives></ref><ref id="cit6"><label>6</label><citation-alternatives><mixed-citation xml:lang="ru">Minocha R., Majumbar R., Minocha S.C. Poliamines and abiotic stress in plants: a complex relationship // Front. Plant Sci. 2014. Vol. 5. Article 175. P. 6–22.</mixed-citation><mixed-citation xml:lang="en">Minocha R., Majumbar R., Minocha S.C. Poliamines and abiotic stress in plants: a complex relationship // Front. Plant Sci. 2014. Vol. 5. Article 175. P. 6–22.</mixed-citation></citation-alternatives></ref><ref id="cit7"><label>7</label><citation-alternatives><mixed-citation xml:lang="ru">Mapelli S., Brambilla I.M., Radyukina N.L., Ivanov Y.V., Kartashov A.V., Reggiani R., Kuznetsov V.V. Free and bound polyamines changes in different plants as a consequence of UV-B light irradiation // Gen. Appl. Plant Physiol. 2008. Vol. 34. N 1–2. P. 55–66.</mixed-citation><mixed-citation xml:lang="en">Mapelli S., Brambilla I.M., Radyukina N.L., Ivanov Y.V., Kartashov A.V., Reggiani R., Kuznetsov V.V. Free and bound polyamines changes in different plants as a consequence of UV-B light irradiation // Gen. Appl. Plant Physiol. 2008. Vol. 34. N 1–2. P. 55–66.</mixed-citation></citation-alternatives></ref><ref id="cit8"><label>8</label><citation-alternatives><mixed-citation xml:lang="ru">Radyukina N.L., Shashukova A.V., Mapelli S., Shevyakova N.I., Kuznetsov V.V. Proline controls the level of polyamines in common sage plants under normal conditions and at UV-B irradiation // Russ. J. Plant Physiol. 2010. Vol. 57. N 3. P. 422–429.</mixed-citation><mixed-citation xml:lang="en">Radyukina N.L., Shashukova A.V., Mapelli S., Shevyakova N.I., Kuznetsov V.V. Proline controls the level of polyamines in common sage plants under normal conditions and at UV-B irradiation // Russ. J. Plant Physiol. 2010. Vol. 57. N 3. P. 422–429.</mixed-citation></citation-alternatives></ref><ref id="cit9"><label>9</label><citation-alternatives><mixed-citation xml:lang="ru">Radyukina N.L., Kartashov A.V., Ivanov Y.V., Shevyakova N.I., Kuznetsov V.V. Functioning of defense systems in halophytes and glycophytes under progressing salinity // Russ. J. Plant. Physiol. 2007. Vol. 54. N 6. P. 806–815.</mixed-citation><mixed-citation xml:lang="en">Radyukina N.L., Kartashov A.V., Ivanov Y.V., Shevyakova N.I., Kuznetsov V.V. Functioning of defense systems in halophytes and glycophytes under progressing salinity // Russ. J. Plant. Physiol. 2007. Vol. 54. N 6. P. 806–815.</mixed-citation></citation-alternatives></ref><ref id="cit10"><label>10</label><citation-alternatives><mixed-citation xml:lang="ru">Son S., Lewis B.A. F ree radical scavenging and antioxidative activity of caffeic acid amide and ester analogues: structure-activity relationship // J. Agric. Food Chem. 2002. Vol. 50. N 3. P. 468–472.</mixed-citation><mixed-citation xml:lang="en">Son S., Lewis B.A. F ree radical scavenging and antioxidative activity of caffeic acid amide and ester analogues: structure-activity relationship // J. Agric. Food Chem. 2002. Vol. 50. N 3. P. 468–472.</mixed-citation></citation-alternatives></ref><ref id="cit11"><label>11</label><citation-alternatives><mixed-citation xml:lang="ru">Alcázar R., Altabella T., Marco F., Bortolotti C., Reymond M., Koncz C., Carrasco P., Tiburcio A.F. Polyamines: molecules with regulatory functions in plant abiotic stress tolerance // Planta. 2010. Vol. 231. N 6. P. 1237–1249.</mixed-citation><mixed-citation xml:lang="en">Alcázar R., Altabella T., Marco F., Bortolotti C., Reymond M., Koncz C., Carrasco P., Tiburcio A.F. Polyamines: molecules with regulatory functions in plant abiotic stress tolerance // Planta. 2010. Vol. 231. N 6. P. 1237–1249.</mixed-citation></citation-alternatives></ref></ref-list><fn-group><fn fn-type="conflict"><p>The authors declare that there are no conflicts of interest present.</p></fn></fn-group></back></article>
