vestnik-bio-msuВестник Московского университета. Серия 16. БиологияVestnik Moskovskogo universiteta. Seriya 16. Biologiya0137-0952Lomonosov Moscow State University, School of Biologyvestnik-bio-msu-497Research ArticleМолекулярная биологияMolecular biologyРОЛЬ БЕЛКА NHP6 В ТРАНСКРИПЦИИ НУКЛЕОСОМ IN VITROROLE OF NHP6 PROTEIN IN TRANSCRIPTION THROUGH A NUCLEOSOME IN VITROХсиехФ. К.HsiehF. K.

Department of Molecular Biology

25 Shattuck St., Boston, MA 02114, USA

науч. сотр. Медицинского факультета Гарвардского университета. Тел.: +1-617-432-1000

Department of Molecular Biology

25 Shattuck St., Boston, MA 02114, USA

hsieh@molbio.mgh.harvard.eduКозловаА. Л.KozlovaA. L.

кафедра биоинженерии, биологический факультет

Россия, 119234, г. Москва, Ленинские горы, д. 1, стр. 12

студентка кафедры молекулярной биологии биологического факультета МГУ. Тел.: 8-495-938-22-91

Department of Bioengineering, School of Biology

1–12 Leninskiye Gory, Moscow, 119234, Russia

mika.lorens@yandex.ruГерасимоваН. С.GerasimovaN. S.

кафедра биоинженерии, биологический факультет

Россия, 119234, г. Москва, Ленинские горы, д. 1, стр. 12

канд. биол. наук, мл. науч. сотр. кафедры биоинженерии биологического факультета МГУ. Тел.: 8-495-938-22-91

Department of Bioengineering, School of Biology

1–12 Leninskiye Gory, Moscow, 119234, Russia

shordome@gmail.comКотоваЕ. Ю.KotovaE.

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науч. сотр. кафедры эпигенетики рака Онкологического центра Фокс Чейз. Тел.: +1-215-728-7014

Cottman Avenue 333, Philadelphia, PA 19111, USA

elena.kotova@fccc.eduФормозаТ.FormosaT.

30 N 1900 E, Salt Lake City, Utah, UT 84132, USA

PhD, проф. кафедры биохимии медицинского факультета университета Юты. Тел: +1-801-581-7201

30 N 1900 E, Salt Lake City, Utah, UT 84132, USA

tim@biochem.utah.eduСтудитскийВ. М.StuditskyV. M.

кафедра биоинженерии, биологический факультет

Россия, 119234, г. Москва, Ленинские горы, д. 1, стр. 12

Cottman Avenue 333, Philadelphia, PA 19111, USA

докт. биол. наук, гл. науч. сотр. кафедры биоинженерии биологического факультета МГУ. Тел. 8-495-938-22-91

Department of Bioengineering, School of Biology

1–12 Leninskiye Gory, Moscow, 119234, Russia

Cottman Avenue 333, Philadelphia, PA 19111, USA

vasily.studitsky@fccc.eduHarvard Medical SchoolСоединённые Штаты АмерикиHarvard Medical SchoolUnited StatesМосковский государственный университет имени М.В. ЛомоносоваРоссияLomonosov Moscow State UniversityRussian FederationCancer Epigenetics Program, Fox Chase Cancer CenterСоединённые Штаты АмерикиCancer Epigenetics Program, Fox Chase Cancer CenterUnited StatesUniversity of Utah, School of MedicineСоединённые Штаты АмерикиUniversity of Utah, School of MedicineUnited StatesМосковский государственный университет имени М.В. Ломоносова Cancer Epigenetics Program, Fox Chase Cancer CenterРоссияLomonosov Moscow State University Cancer Epigenetics Program, Fox Chase Cancer CenterRussian Federation201720102017724253257Copyright © Хсиех Ф.К., Козлова А.Л., Герасимова Н.С., Котова Е.Ю., Формоза Т., Студитский В.М., 20172017Хсиех Ф.К., Козлова А.Л., Герасимова Н.С., Котова Е.Ю., Формоза Т., Студитский В.М.Hsieh F.K., Kozlova A.L., Gerasimova N.S., Kotova E., Formosa T., Studitsky V.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestnik-bio-msu.elpub.ru/jour/article/view/497

Nhp6 – это небольшой белок дрожжей, неспецифично связывающий ДНК. Ранее было показано, что Nhp6 входит в состав нескольких комплексов (в том числе комплекса FACT), присутствует на многих дрожжевых промоторах и транскрибируемых участках генов in vivo и участвует в процессе дестабилизации структуры нуклеосом in vitro. В нашей лаборатории был изучен фактор FACT и показана его роль в трaнскрипции хроматина эукариотической РНК-полимеразой 2 in vitro, однако роль белка Nhp6 в транскрипции ранее не была исследована. В данной работе мы изучили влияние этого белка на транскрипцию нуклеосом эукариотической РНК-полимеразой 2 и показали, что он увеличивает эффективность транскрипции в нескольких положениях на нуклеосомной ДНК, в первую очередь – участка в положении +(11–17) в нуклеосоме. Предложена модель действия Nhp6, предполагающая стабилизацию транзитного отворачивания ДНК от октамера гистонов в процессе транскрипции хроматина.

Nhp6 is a small yeast protein, which binds DNA nonspecifically. It was shown that Nhp6 is a part of several protein complexes (including FACT complex) and is presented on many yeast promoters and transcribed regions of genes in vivo. It also participates in the process of destabilizing the structure of nucleosomes in vitro. In our laboratory, we studied FACT complex and showed its role in the transcription of eukaryotic RNA-polymerase 2 in vitro, but the role of the Nhp6 protein in transcription has not been studied previously. In this paper, we describe the effect of Nhp6 protein on transcription through a nucleosome by eukaryotic RNA-polymerase 2 and show that Nhp6 protein increases the transcription efficiency at several positions on the nucleosomal DNA, primarily the transcription at the positions +(11–17) in the nucleosome. We proposed a model of the action of Nhp6 during transcription through chromatin, suggesting the stabilization of DNA transiently uncoiled from the octamer during this process.

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The authors declare that there are no conflicts of interest present.