vestnik-bio-msuВестник Московского университета. Серия 16. БиологияVestnik Moskovskogo universiteta. Seriya 16. Biologiya0137-0952Lomonosov Moscow State University, School of Biologyvestnik-bio-msu-522Research ArticleГенетикаGeneticsСОЗДАНИЕ ST/J/V-ГЕНОМ-СПЕЦИФИЧНОГО МОЛЕКУЛЯРНОГО МАРКЕРА НА ОСНОВЕ ПОЛИМОРФИЗМА ЛОКУСОВ 5S-рДНК THINOPYRUM BESSARABICUM, PSEUDOROEGNERIA SPICATA И DASYPYRUM VILLOSUMDEVELOPMENT OF THE ST/J/V GENOME SPECIFIC MOLECULAR MARKER BASED ON 5S RDNA POLYMORPHISM IN THINOPYRUM BESSARABICUM, PSEUDOROEGNERIA SPICATA AND DASYPYRUM VILLOSUMАлександровО. С.AlexandrovO. S.

Александров Олег Сергеевич – канд. биол. наук, ст. науч. сотр. Центра молекулярной биотехнологии РГАУ – МСХА имени К.А. Тимирязева.

Россия, 127550, г. Москва, ул. Тимирязевская, д. 49;

Timiryazevskaya st. 49, Moscow, 127550, Russia

olegsandrov@gmail.comДивашукМ. Г.DivashukM. G.

Дивашук Михаил Георгиевич – канд. биол. наук, ст. науч. сотр. Центра молекулярной биотехнологии РГАУ – МСХА имени К.А. Тимирязева, зав. лаб. диагностики патогенов растений.

Россия, 127550, г. Москва, ул. Тимирязевская, д. 49;Россия, 127550, г. Москва, ул. Тимирязевская, д. 42

Timiryazevskaya st. 49, Moscow, 127550, Russia;Timiryazevskaya st. 42, Moscow, 12755, Russia

divashuk@gmail.comКарловГ. И.KarlovG. I.

Карлов Геннадий Ильич – докт. биол. наук, проф., член-корр. РАН, и. о. директора Всероссийского научно-исследовательского института сельскохозяйственной биотехнологии, руководитель Центра молекулярной биотехнологии РГАУ – МСХА имени К.А. Тимирязева.

Россия, 127550, г. Москва, ул. Тимирязевская, д. 49;Россия, 127550, г. Москва, ул. Тимирязевская, д. 42

Timiryazevskaya st. 49, Moscow, 127550, Russia;Timiryazevskaya st. 42, Moscow, 12755, Russia

karlovg@gmail.ruЦентр молекулярной биотехнологии Российского государственного аграрного университета - МСХА имени К.А. ТимирязеваРоссияCentre for Molecular Biotechnology, Russian State Agrarian University-Moscow Timiryazev Agricultural AcademyRussian FederationЦентр молекулярной биотехнологии Российского государственного аграрного университета - МСХА имени К.А. Тимирязева; Всероссийский научно-исследовательский институт сельскохозяйственной биотехнологииРоссияCentre for Molecular Biotechnology, Russian State Agrarian University-Moscow Timiryazev Agricultural Academy; All-Russia Research Institute of Agricultural BiotechnologyRussian Federation2018230120187312228Copyright © Александров О.С., Дивашук М.Г., Карлов Г.И., 20182018Александров О.С., Дивашук М.Г., Карлов Г.И.Alexandrov O.S., Divashuk M.G., Karlov G.I.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestnik-bio-msu.elpub.ru/jour/article/view/522

Нетранскрибируемые спейсеры (non-transcribed spacers, NTS) 5S-рДНК часто являются полиморфными у достаточно близких видов и даже внутри одного генома. У представителей трибы Triticeae – Thinopyrum bessarabicum, Pseudoroegneria spicata и Dasypyrum villosum – был выявлен полиморфизм по NTS 5S-рДНК между геномами St, J и V. На основе данного полиморфизма была осуществлена разработка молекулярно-генетического маркера, позволяющего идентифицировать эти три генома. Пара праймеров была подобрана к последовательностям консервативных областей NTS 5S-рДНК данных геномов.Длина амплифицируемого фрагмента составила 158 п.о. для V-генома, 171 п.о. для St-генома и 172 п.о. для J-генома. Фрагменту St-генома также свойственно наличие сайта узнавания для эндонуклеазы рестрикции SmiM I, что позволяет отличать его от фрагмента J-генома, в котором этот сайт отсутствует. Разработанный маркер показал высокую эффективность при верификации образцов коллекции и изучении аллополиплоидов.

Non-transcribed spacers (NTS) of 5S rDNA are often polymorphic in closely related species and even in the same genome. The polymorphism of 5S rDNA NTS was shown between genomes St, J and V of Triticeae species Thinopyrum bessarabicum, Pseudoroegneria spicata, and Dasypyrum villosum, respectively. A molecular genetic marker was designed based on the 5S rDNA NTS polymorphism that allows identification of the St, J and V genomes. We designed a pair of primers that correspond to the conserved regions of 5S rDNA NTS between the genomes studied. The PCR amplicon length is 158 bp for V, 171 bp for St and 172 bp for J genome. The fragment of St genome is characterized by SmiM I restriction site that enables its differentiation from J genome fragment that lacks this site. The developed marker showed its efficiency for verification of germplasm accessions and the study of allopolyploids.

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The authors declare that there are no conflicts of interest present.