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<article article-type="research-article" dtd-version="1.3" xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">vestnik-bio-msu</journal-id><journal-title-group><journal-title xml:lang="ru">Вестник Московского университета. Серия 16. Биология</journal-title><trans-title-group xml:lang="en"><trans-title>Vestnik Moskovskogo universiteta. Seriya 16. Biologiya</trans-title></trans-title-group></journal-title-group><issn pub-type="ppub">0137-0952</issn><publisher><publisher-name>Lomonosov Moscow State University,  School of Biology</publisher-name></publisher></journal-meta><article-meta><article-id custom-type="elpub" pub-id-type="custom">vestnik-bio-msu-623</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research Article</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="ru"><subject>Биофизика</subject></subj-group><subj-group subj-group-type="section-heading" xml:lang="en"><subject>Biophysics</subject></subj-group></article-categories><title-group><article-title>ВОЗМОЖНОСТИ ОПТИЧЕСКОГО МОНИТОРИНГА ФОСФОРНОГО ГОЛОДАНИЯ В СУСПЕНЗИОННЫХ КУЛЬТУРАХ МИКРОВОДОРОСЛИ CHLORELLA VULGARIS IPPAS C-1 (CHLOROPHYCEAE)</article-title><trans-title-group xml:lang="en"><trans-title>POSSIBILITIES OF OPTICAL MONITORING OF PHOSPHORUS STARVATION IN SUSPENSIONS OF THE MICROALGA CHLORELLA VULGARIS IPPAS C-1 (CHLOROPHYCEAE)</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Кузнецов</surname><given-names>А. Г.</given-names></name><name name-style="western" xml:lang="en"><surname>Kuznetsov</surname><given-names>A. G.</given-names></name></name-alternatives><bio xml:lang="ru"><p>аспирант кафедры биофизики биологического факультета,</p><p>119234, г. Москва, Ленинские горы, д. 1, стр. 12</p></bio><bio xml:lang="en"><p>Departments of Biophysics, Faculty of Biology,</p><p>119234, Moscow, Leninskiye Gory 1–12</p></bio><email xlink:type="simple">79257291630@yandex.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Погосян</surname><given-names>С. И.</given-names></name><name name-style="western" xml:lang="en"><surname>Pogosyan</surname><given-names>S. I.</given-names></name></name-alternatives><bio xml:lang="ru"><p>докт. биол. наук, проф. кафедры биофизики биологического факультета,</p><p>119234, г. Москва, Ленинские горы, д. 1, стр. 12</p></bio><bio xml:lang="en"><p>Departments of Biophysics, Faculty of Biology,</p><p>119234, Moscow, Leninskiye Gory 1–12</p></bio><email xlink:type="simple">pogosyan@biophys.msu.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Конюхов</surname><given-names>И. В.</given-names></name><name name-style="western" xml:lang="en"><surname>Konyukhov</surname><given-names>I. V.</given-names></name></name-alternatives><bio xml:lang="ru"><p>канд. биол. наук, вед. науч. сотр. кафедры биофизики биологического факультета,</p><p>119234, г. Москва, Ленинские горы, д. 1, стр. 12</p></bio><bio xml:lang="en"><p>Departments of Biophysics, Faculty of Biology, </p><p>119234, Moscow, Leninskiye Gory 1–12</p></bio><email xlink:type="simple">vanka_kon@gmail.com</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Васильева</surname><given-names>С. Г.</given-names></name><name name-style="western" xml:lang="en"><surname>Vasilieva</surname><given-names>S. G.</given-names></name></name-alternatives><bio xml:lang="ru"><p>канд. биол. наук, науч. сотр. кафедры биоинженерии биологического факультета,</p><p>119234, г. Москва, Ленинские горы, д. 1, стр. 12</p></bio><bio xml:lang="en"><p>Departments of Bioengineering, Faculty of Biology,</p><p>119234, Moscow, Leninskiye Gory 1–12;</p></bio><email xlink:type="simple">vankat2009@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Лукьянов</surname><given-names>А. А.</given-names></name><name name-style="western" xml:lang="en"><surname>Lukyanov</surname><given-names>A. A.</given-names></name></name-alternatives><bio xml:lang="ru"><p>канд. биол. наук, науч. сотр. кафедры биоинженерии биологического факультета,</p><p>119234, г. Москва, Ленинские горы, д. 1, стр. 12</p></bio><bio xml:lang="en"><p>Departments of Bioengineering, Faculty of Biology,</p><p>119234, Moscow, Leninskiye Gory 1–12</p></bio><email xlink:type="simple">loockart@mail.ru</email><xref ref-type="aff" rid="aff-1"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Зотов</surname><given-names>В. С.</given-names></name><name name-style="western" xml:lang="en"><surname>Zotov</surname><given-names>V. S.</given-names></name></name-alternatives><bio xml:lang="ru"><p>канд. биол. наук, руководитель группы,</p><p>119071, г. Москва, Ленинский проспект, д. 33, стр. 2</p></bio><bio xml:lang="en"><p>119071 Moscow, Leninskii Prospekt 33–2</p></bio><email xlink:type="simple">algo.consortium@gmail.com</email><xref ref-type="aff" rid="aff-2"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Недбал</surname><given-names>Л.</given-names></name><name name-style="western" xml:lang="en"><surname>Nedbal</surname><given-names>L.</given-names></name></name-alternatives><bio xml:lang="ru"><p>доктор наук (Ph.D.), ст. исследователь,</p><p>52428, г. Юлих, Вилем-Йонен штрассе, 2</p></bio><bio xml:lang="en"><p>52428 Jülich, Leo-Brandt str</p></bio><email xlink:type="simple">l.nedbal@fz-juelich.de</email><xref ref-type="aff" rid="aff-3"/></contrib><contrib contrib-type="author" corresp="yes"><name-alternatives><name name-style="eastern" xml:lang="ru"><surname>Соловченко</surname><given-names>А. Е.</given-names></name><name name-style="western" xml:lang="en"><surname>Solovchenko</surname><given-names>A. E.</given-names></name></name-alternatives><bio xml:lang="ru"><p>докт. биол. наук, проф. кафедры биоинженерии биологического факультета;</p><p>вед. науч. сотр. Евразийского центра по продовольственной безопасности, </p><p>119234, г. Москва, Ленинские горы, д. 1, стр. 12</p><p> </p></bio><bio xml:lang="en"><p>Departments of Bioengineering, Faculty of Biology,</p><p>119234, Moscow, Leninskiye Gory, 1–12</p></bio><email xlink:type="simple">solovchenko@mail.bio.msu.ru</email><xref ref-type="aff" rid="aff-4"/></contrib></contrib-group><aff-alternatives id="aff-1"><aff xml:lang="ru"><institution>Московский государственный университет имени М.В. Ломоносова</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Lomonosov Moscow State University</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-2"><aff xml:lang="ru"><institution>ФИЦ Биотехнологии РАН</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Federal Research Centre “Fundamentals of Biotechnology” RAS</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-3"><aff xml:lang="ru"><institution>Институт биологии и геологии (IBG-2), Исследовательский центр Юлих (FZJ)</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Institute of Bio- and Geosciences 2: Plant Sciences (IBG-2), Forschungszentrum J lich (FZJ)</institution><country>Russian Federation</country></aff></aff-alternatives><aff-alternatives id="aff-4"><aff xml:lang="ru"><institution>Московский государственный университет имени М.В. Ломоносова;&#13;
Евразийский центр по продовольственной безопасности</institution><country>Россия</country></aff><aff xml:lang="en"><institution>Lomonosov Moscow State University;&#13;
Euarsian Center for Food Security</institution><country>Russian Federation</country></aff></aff-alternatives><pub-date pub-type="collection"><year>2018</year></pub-date><pub-date pub-type="epub"><day>22</day><month>08</month><year>2018</year></pub-date><volume>73</volume><issue>3</issue><fpage>146</fpage><lpage>152</lpage><permissions><copyright-statement>Copyright &amp;#x00A9; Кузнецов А.Г., Погосян С.И., Конюхов И.В., Васильева С.Г., Лукьянов А.А., Зотов В.С., Недбал Л., Соловченко А.Е., 2018</copyright-statement><copyright-year>2018</copyright-year><copyright-holder xml:lang="ru">Кузнецов А.Г., Погосян С.И., Конюхов И.В., Васильева С.Г., Лукьянов А.А., Зотов В.С., Недбал Л., Соловченко А.Е.</copyright-holder><copyright-holder xml:lang="en">Kuznetsov A.G., Pogosyan S.I., Konyukhov I.V., Vasilieva S.G., Lukyanov A.A., Zotov V.S., Nedbal L., Solovchenko A.E.</copyright-holder><license xml:lang="ru" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>Данная работа распространяется под лицензией Creative Commons Attribution 4.0.</license-p></license><license xml:lang="en" license-type="creative-commons-attribution" xlink:href="https://creativecommons.org/licenses/by/4.0/" xlink:type="simple"><license-p>This work is licensed under a Creative Commons Attribution 4.0 License.</license-p></license></permissions><self-uri xlink:href="https://vestnik-bio-msu.elpub.ru/jour/article/view/623">https://vestnik-bio-msu.elpub.ru/jour/article/view/623</self-uri><abstract><p>Исследования влияния обеспеченности неорганическим фосфором (Pi ), важным биогенным элементом, на рост и физиологические параметры одноклеточных водорослей важны для определения динамики численности и продуктивности фитопланктона в природных экосистемах и промышленных системах с целью культивирования этих организмов. Затруднения при проведении таких исследований связаны со сложной кинетикой поглощения Pi клетками и способностью микроводорослей к внутриклеточному запасанию фосфора. В этой связи необходимы эффективные способы экспресс-мониторинга состояния культур микроводорослей. Данным критериям отвечают методы, основанные на регистрации оптических свойств клеток, таких как поглощение и рассеяние света культурами и флуоресценции содержащегося в клетках хлорофилла. В настоящей статье описаны результаты мониторинга культуры зеленой микроводоросли Chlorella vulgaris IPPAS C-1, культивируемой в среде, не содержащей фосфора. Установлено, что и оптические (поглощение света в полосах фотосинтетических пигментов – хлорофиллов и каротиноидов), и люминесцентные (переменная флуоресценция хлорофилла) параметры отражают состояние культуры. При регистрации оптических свойств необходима коррекция вклада светорассеяния в общее ослабление света суспензиями клеток микроводорослей, при этом сам по себе уровень светорассеяния является точной мерой общего количества взвешенных частиц в суспензии. Однако мониторинг культур, содержащих значительное количество светорассеивающих частиц без фотосинтетических пигментов (таких как гетеротрофные бактерии) затруднен. Для подобных культур оптимальным является использование переменной флуоресценции – например, параметра Fv/Fm, отражающего максимальную фотохимическую эффективность фотосистемы II.</p></abstract><trans-abstract xml:lang="en"><p>Studies of the impact of inorganic phosphorus (Pi ), an important nutrient, on the growth and physiological parameters of single-celled algae are important for investigations of the dynamics of phytoplankton abundance and productivity in natural ecosystems as well as in industrial systems for the cultivation of microalgae. Difficulties in carrying out such studies are associated with the complex kinetics of Pi uptake by and the ability of microalgae to store phosphorus in their cells. This situation necessitates the efficient methods for express monitoring of microalgal cultures such as the methods based on the registration of optical properties of cells, such as absorption and scattering of light and fluorescence of chlorophyll contained in the cells. Here, we describe the results of monitoring the cultures of a chlorophyte Chlorella vulgaris IPPAS C-1, starving for phosphorus. It was found that both optical (light absorption in the bands of the key pigments–chlorophylls and carotenoids) and luminescent (variable fluorescence of chlorophyll) parameters reflect closely the culture condition. The correction for the contribution of light scattering to the overall extinction of light by microalgal cell suspensions turned to be necessary. At the same time, the light scattering signal is an accurate measure of the total number of suspended particles in the suspension. However, it is difficult to monitor via optical absorption the samples with abundant light-scattering particles lacking the photosynthetic pigments (such as heterotrophic bacteria). For such cultures, the using of variable fluorescence-based parameter Fv/Fm reflecting the maximum photochemical efficiency of the photosystem II is advisable.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>микроводоросли</kwd><kwd>светорассеяние</kwd><kwd>фосфорное голодание</kwd><kwd>флуоресценция хлорофилла</kwd><kwd>фотобиореактор</kwd><kwd>Chlorella vulgaris</kwd></kwd-group><kwd-group xml:lang="en"><kwd>microalgae</kwd><kwd>light scattering</kwd><kwd>phosphorus starvation</kwd><kwd>chlorophyll fluorescence</kwd><kwd>photobioreactor</kwd><kwd>Chlorella vulgaris</kwd></kwd-group><funding-group><funding-statement xml:lang="ru">Минобрнауки</funding-statement></funding-group></article-meta></front><back><ref-list><title>References</title><ref id="cit1"><label>1</label><citation-alternatives><mixed-citation xml:lang="ru">Grossman A.R., Aksoy M. Algae in a phosphorus-limited landscape // Annual plant reviews. Vol. 48. Phosphorus metabolism in plants / Eds. W. Plaxton and H. Lambers. 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