vestnik-bio-msuВестник Московского университета. Серия 16. БиологияVestnik Moskovskogo universiteta. Seriya 16. Biologiya0137-0952Lomonosov Moscow State University, School of Biologyvestnik-bio-msu-624Research ArticleГеронтологияGerontologyУХУДШЕНИЕ ЖИЗНЕСПОСОБНОСТИ ТРАНСФОРМИРОВАННЫХ КЛЕТОК КИТАЙСКОГО ХОМЯЧКА В НЕПЕРЕСЕВАЕМОЙ КУЛЬТУРЕ ПОД ВЛИЯНИЕМ ЭКЗОГЕННОГО ОКИСЛЕННОГО ГУАНОЗИДА ПРОЯВЛЯЕТСЯ ТОЛЬКО В СТАЦИОНАРНОЙ ФАЗЕ РОСТАIMPAIRMENT OF THE VIABILITY OF TRANSFORMED CHINESE HAMSTER CELLS IN A NONSUBCULTURED CULTURE UNDER THE INFLUENCE OF EXOGENOUS OXIDIZED GUANOSIDE IS MANIFESTED ONLY IN THE STATIONARY PHASE OF GROWTHМоргуноваГ. В.MorgunovaG. V.

Сектор эволюционной цитогеронтологии, биологический факультет, 

119234, Москва, Ленинские горы, д. 1, стр. 12

Evolutionary Cytogerontology Sector, School of Biology, 

Leninskiye gory 1–12, Moscow, 119234

morgunova@mail.bio.msu.ruКлебановА. А.KlebanovA. A.

Сектор эволюционной цитогеронтологии, биологический факультет, 

119234, Москва, Ленинские горы, д. 1, стр. 12

Evolutionary Cytogerontology Sector, School of Biology, 

Leninskiye gory 1–12, Moscow, 119234

Московский государственный университет имени М.В. ЛомоносоваРоссияLomonosov Moscow State UniversityRussian Federation201822082018733153159Copyright © Моргунова Г.В., Клебанов А.А., 20182018Моргунова Г.В., Клебанов А.А.Morgunova G.V., Klebanov A.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestnik-bio-msu.elpub.ru/jour/article/view/624

Несмотря на то, что продукты окисления нуклеотидов и нуклеозидов являются маркёрами окислительного стресса, всё чаще стали появляться сообщения, свидетельствующие о парадоксальной способности этих соединений защищать клетки от повреждающего воздействия активных форм кислорода. Среди всех азотистых оснований наиболее восприимчив к влиянию окислительного стресса гуанин, поэтому чаще остальных окисляются гуанозины. В настоящей работе исследовано влияние экзогенного 8-оксо-2'- дезоксигуанозина на кинетику роста и “стационарного старения” (накопление “возрастных” изменений клеток при замедлении скорости размножения в пределах одного пассажа и дальнейшем их пребывании в стационарной фазе роста) непересеваемой культуры трансформированных клеток китайского хомячка. Показано, что нуклеозид быстро поглощается клетками из среды, однако он никак не влияет на кинетику роста культуры и ухудшает жизнеспособность клеток, находящихся в поздней стационарной фазе. Таким образом, не было обнаружено митогенного или геропротекторного эффекта 8-оксо-2'- дезоксигуанозина.

Despite the fact that oxidation products of nucleotides and nucleosides are markers of oxidative stress, reports of the paradoxical ability of these compounds to protect cells from the harmful effects of reactive oxygen species began to appear more often. Among all nitrogenous bases, guanine is most susceptible to the influence of oxidative stress, therefore, guanosine is oxidized more often than other bases. In the present work, the effect of exogenous 8-oxo-2'-deoxyguanosine on the growth and “stationary phase aging” (accumulation of “age” changes in cultured cells during cell proliferation slowing down within a single passage and subsequent “aging” in the stationary phase of growth) of a nonsubcultured culture of transformed Chinese hamster cells was studied. We showed that the nucleoside is rapidly absorbed by the cells from the medium, but it does not affect the growth of the culture, and in the late stationary phase of growth impairs the viability of the cells. Thus, no mitogenic or geroprotective effect of 8-oxo-2'-deoxyguanosine was found.

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The authors declare that there are no conflicts of interest present.