vestnik-bio-msuВестник Московского университета. Серия 16. БиологияVestnik Moskovskogo universiteta. Seriya 16. Biologiya0137-0952Lomonosov Moscow State University, School of Biologyvestnik-bio-msu-837Research ArticleОРИГИНАЛЬНОЕ ИССЛЕДОВАНИЕRESEARCH ARTICLEСекреция микромицетами протеиназ с активностью, подобной активности белков системы гемостазаSecretion of proteinase with activity that is similar to activity of proteins of the hemostatic system by micromycetesЛукьяноваА. А.LukianovaA. A.

аспирант кафедры микробиологии биологического факультета,

119234, г. Москва, Ленинские горы, д. 1, стр. 12

Department of Microbiology, Faculty of Biology,

Leninskiye gory 1–12, Moscow, 119234

a.al.lukianova@gmail.comКорниенкоЕ. И.KornienkoE. I.

мл. науч. сотр. кафедры микробиологии биологического факультета,

119234, г. Москва, Ленинские горы, д. 1, стр. 12

Department of Microbiology, Faculty of Biology,

Leninskiye gory 1–12, Moscow, 119234

aljnka-93@mail.ruВиганП. А.VigandP. A.

магистр,

Route de l’Orme aux Merisiers, RD 128, 91190, Saint-Aubin

Route de l’Orme aux Merisiers, RD 128, 91190, Saint-Aubin

pauline-vigand@hotmail.frКрейерВ. Г.KreyerV. G.

канд. биол. наук, науч. сотр. кафедры микробиологии биологического факультета,

119234, г. Москва, Ленинские горы, д. 1, стр. 12

Department of Microbiology, Faculty of Biology, 

Leninskiye gory 1–12, Moscow, 119234

vkreyer@yandex.ruКураковА. В.KurakovA. V.

докт. биол. наук, зав. кафедрой микологии и альгологии биологического факультета,

119234, г. Москва, Ленинские горы, д. 1, стр. 12

Department of Mycology and Algology, Faculty of Biology, 

Leninskiye gory 1–12, Moscow, 119234

kurakov57@mail.ruОсмоловскийА. А.OsmolovskiyA. A.

канд. биол. наук, ст. преп. кафедры микробиологии биологического факультета,

119234, г. Москва, Ленинские горы, д. 1, стр. 12

Department of Microbiology, Faculty of Biology,

Leninskiye gory 1–12, Moscow, 119234

aosmol@mail.ruМосковский государственный университет имени М.В. ЛомоносоваРоссияLomonosov Moscow State UniversityRussian FederationParis-Sacley UniversityФранцияParis-Sacley UniversityFrance2020100320207513742Copyright © Лукьянова А.А., Корниенко Е.И., Виган П.А., Крейер В.Г., Кураков А.В., Осмоловский А.А., 20202020Лукьянова А.А., Корниенко Е.И., Виган П.А., Крейер В.Г., Кураков А.В., Осмоловский А.А.Lukianova A.A., Kornienko E.I., Vigand P.A., Kreyer V.G., Kurakov A.V., Osmolovskiy A.A.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestnik-bio-msu.elpub.ru/jour/article/view/837

С использованием хромогенных пептидных субстратов изучена протеолитическая активность по отношению к белкам системы гемостаза у 8 микромицетов, относящихся к разным эколого-трофическим группам: фито-, немато-, мико- и энтомопатогенам, а также патогенам человека. Показано, что исследованные микромицеты секретируют протеазы с широкой субстратной специфичностью, однако среди них есть продуценты с выраженной плазминоподобной, урокиназной (Arthrobotrys longa 1, Sarocladium strictum 203) и подобной тканевому активатору плазминогена активностью (Purpureocillium lilacinum k1). Наиболее высокоактивным продуцентом протеаз с фибринолитической и активаторной к плазминогену активностью оказался микромицет S. strictum 203. Активаторная к плазминогену активность протеиназ, образуемых S. strictum 203, составляет около 50% от фибринолитической, что делает его перспективным продуцентом фибринолитических ферментов.

Using the chromogenic peptide substrates, proteolytic activity against proteins of the hemostasis system was studied in 9 micromycetes belonging to different ecological-trophic groups: phyto-, nemato-, myco- and entomopathogens, as well as human pathogens. It is shown that the investigated micromycetes secrete proteases with broad substrate specificity, but among them there are producers with pronounced both plasmin-like and urokinase (Arthrobotrys longa 1, Sarocladium strictum 203) also as TAP-like (Purpureocillium lilacinum k1) activity. The most active producer of proteases with fibrinolytic and activator activity to plasminogen activity was S. strictum 203 micromycete. The activity of proteinases formed by S. strictum 203 is about 50% of the fibrinolytic activity of plasminogen, which makes it a promising producer of fibrinolytic enzymes.

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The authors declare that there are no conflicts of interest present.