vestnik-bio-msuВестник Московского университета. Серия 16. БиологияVestnik Moskovskogo universiteta. Seriya 16. Biologiya0137-0952Lomonosov Moscow State University, School of Biologyvestnik-bio-msu-898Research ArticleОРИГИНАЛЬНОЕ ИССЛЕДОВАНИЕRESEARCH ARTICLEМолекулярный анализ генов сигнального пути гиббереллинов у ржи посевной (Secale cereale L.)Molecular analysis of the gibberellin signaling pathway genes in cultivated rye (Secale cereale L.)БаженовМ. С.BazhenovM. S.

Баженов Михаил Сергеевич - канд. биол. наук, ст. науч. сотр. лаборатории прикладной геномики и частной селекции сельскохозяйственных растений

127550, г. Москва, ул. Тимирязевская, д. 42, тел.: 8-499-976-65-44

Laboratory of Applied Genomics and Breeding of Agricultural Plants

Timiryazevskaya st. 42, Moscow, 127550

mikhabazhenov@gmail.comЧерноокА. Г.ChernookA. G.

Черноок Анастасия Геннадиевна - мл. науч. сотр. лаборатории прикладной геномики и частной селекции сельскохозяйственных растений

127550, г. Москва, ул. Тимирязевская, д. 42, тел.: 8-499-976-65-44

Laboratory of Applied Genomics and Breeding of Agricultural Plants

Timiryazevskaya st. 42, Moscow, 127550

irbis-sibri@yandex.ruДивашукМ. Г.DivashukM. G.

Дивашук Михаил Георгиевич - канд. биол. наук, вед. науч. сотр., зав. лаборатории прикладной геномики и частной селекции сельскохозяйственных растений, доцент кафедры генетики, селекции и семеноводства

 127550, г. Москва, ул. Тимирязевская, 127550, тел.: 8-499-976-65-44

Laboratory of Applied Genomics and Breeding of Agricultural Plants, Center for Molecular Biotechnology

Timiryazevskaya st. 42, Moscow, 127550

Timiryazevskaya st. 49, Moscow, 127550

divashuk@gmail.comВсероссийский научно-исследовательский институт сельскохозяйственной биотехнологииРоссияAll-Russia Research Institute of Agricultural BiotechnologyRussian FederationВсероссийский научно-исследовательский институт сельскохозяйственной биотехнологии; Российский государственный аграрный университет - МСХА имени К.А. ТимирязеваРоссияAll-Russia Research Institute of Agricultural Biotechnology; Russian State Agrarian University - Moscow Timiryazev Agricultural AcademyRussian Federation202031072020753153157Copyright © Баженов М.С., Черноок А.Г., Дивашук М.Г., 20202020Баженов М.С., Черноок А.Г., Дивашук М.Г.Bazhenov M.S., Chernook A.G., Divashuk M.G.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestnik-bio-msu.elpub.ru/jour/article/view/898

Гены сигнального пути гиббереллинов, кодирующие DELLA-белок и рецептор гиббереллинов GID1, были секвенированы у нескольких сортов (Альфа, Валдай, Орловская 9, Прача) и одной линии ржи (ЕМ-1) с применением методов нового поколения. Выявленные в результате несколько аллелей этих генов различаются преимущественно однонуклеотидными полиморфизмами, реже – инсерциями и делециями. Бо́льшая часть обнаруженных мутаций оказались синонимичными либо приходящимися на некодирующие регионы генов. Изменения в аминокислотной последовательности белков, связанные с прочими мутациями, вероятно, являются функционально-нейтральными. Мутации, аналогичные пшеничным аллелям гиббереллин-нечувствительной короткостебельности, обнаружены не были.

Gibberellin signaling pathway genes encoding the DELLA protein and gibberellin receptor GID1 were sequenced in several varieties (Alpha, Valdai, Orlovskaya 9, Pracha) and one line of rye (EM-1) using next generation methods. The revealed multiple alleles of these genes differ mainly in single-nucleotide polymorphisms, and less frequently in insertions and deletions. Most of the detected mutations turned out to be synonymous or located in the non-coding regions of the genes. Changes in the amino acid sequences of proteins associated with other mutations are probably functionally neutral. Mutations similar to wheat reduced-height gibberellin-insensitive alleles were not detected.

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The authors declare that there are no conflicts of interest present.