References

vestnik-bio-msu

Вестник Московского университета. Серия 16. Биология

Vestnik Moskovskogo universiteta. Seriya 16. Biologiya

0137-0952

Lomonosov Moscow State University, School of Biology

vestnik-bio-msu-933

Research Article

ОБЗОР

REVIEW

Клетки китайского хомячка в биотехнологических и геронтологических исследованиях

Chinese hamster cells in biotechnological and gerontological research

https://orcid.org/0000-0002-5259-0861

Моргунова

Г. В.

Morgunova

G. V.

Моргунова Галина Васильевна – канд. биол. наук, науч. сотр. сектора эволюционной цитогеронтологии биологического факультета МГУ

119234, г. Москва, Ленинские горы, д. 1, стр. 12

Тел.: 8-495-939-15-90

morgunova@mail.bio.msu.ru

Сектор эволюционной цитогеронтологии, биологический факультет, Московский государственный университет имени М.В. ЛомоносоваРоссияEvolutionary Cytogerontology Sector, School of Biology, Lomonosov Moscow State UniversityRussian Federation

2020

06112020

754237243

2020

Моргунова Г.В.

Morgunova G.V.

Данная работа распространяется под лицензией Creative Commons Attribution 4.0.

This work is licensed under a Creative Commons Attribution 4.0 License.

https://vestnik-bio-msu.elpub.ru/jour/article/view/933

Доступно обновление статьиОдним из наиболее часто используемых в геронтологии модельных объектов являются дрожжи, в первую очередь – Saccharomyces cerevisiae. Накоплено значительное количество данных, позволяющих считать, что в претерпевающих хронологическое, или «стационарное», старение дрожжах возникают нарушения, сходные с возрастными нарушениями в клетках многоклеточного организма. Однако дрожжи, как и любые объекты исследований, не лишены недостатков – в частности, они, хотя и являются эукариотами, в эволюционном плане отстоят далеко от млекопитающих, что накладывает ограничение на изучение у дрожжей неконсервативных метаболических путей. В некоторых случаях в экспериментах с хронологической моделью лучше использовать клетки млекопитающих – например, клетки китайского хомячка. Они широко используются в промышленности для получения моноклональных антител и рекомбинантных белков. Значительная доля этих продуктов образуется после остановки пролиферации, которая инициирует хронологическое старение культуры. Накопленные данные об особенностях метаболизма клеток, роста культуры и продолжительности ее функциональной жизни являются крайне ценными для геронтологов. Обмен информацией между двумя этими направлениями – биотехнологическим и геронтологическим – будет полезен обеим сторонам.

A Correction to this article is availableOne of the most frequently used model objects in gerontology is yeast, primarily Saccharomyces cerevisiae. A significant amount of data has been accumulated, allowing to believe that in yeast undergoing chronological or “stationary phase” aging, damage is similar to age-related lesions in the cells of multicellular organism. However, studies on yeast, like on any objects, are not without drawbacks; in particular, although yeasts are eukaryotes, in terms of evolution they are far from mammals, which impose a limitation on the studies of non-conservative metabolic pathways in yeast. In some cases, mammalian cells are better for chronological model experiments, for example, Chinese hamster cells. They are actively used in industry for the manufacturing of monoclonal antibodies and recombinant proteins. A significant proportion of these products are produced after cessation of proliferation which initiates chronological aging of the culture. The accumulated data on the features of the function of cell metabolism, growth, culture and the duration of its functional activity are extremely valuable for gerontologists. The exchange of information between these two branches – biotechnological and gerontological – will be beneficial to both parties.

клеточное старениеклеточные культуры«стационарное старение»хронологическое старениеметаболизмлактатэффект Варбурга

cell agingcell cultures“stationary phase aging”chronological agingmetabolismlactateWarburg effect

Работа выполнена в рамках государственного задания МГУ, ч. 2 (фундаментальные научные исследования, № АААА-А16-116021660098-8).

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The authors declare that there are no conflicts of interest present.