vestnik-bio-msuВестник Московского университета. Серия 16. БиологияVestnik Moskovskogo universiteta. Seriya 16. Biologiya0137-0952Lomonosov Moscow State University, School of Biologyvestnik-bio-msu-940Research ArticleКРАТКОЕ СООБЩЕНИЕSHORT COMMUNICATIONСтруктура оболочек вокруг метацеркарий трематод Podocotyle atomon (Rudolphi, 1802) во втором промежуточном хозяинеStructure of the envelopes that are formed around metacercariae of the trematode Podocotyle atomon (Rudolphi, 1802) in its second intermediate hosthttps://orcid.org/0000-0002-9497-584XСкоробреховаЕ. М.SkorobrekhovaE. M.

Скоробрехова Екатерина Михайловна – науч. сотр. лаборатории экологии гельминтов

685000, г. Магадан, ул. Портовая, д. 18

Тел.: 8-4132-634-463 

Portovaya str., 18, Magadan, 685000

skorobrechova@mail.ruИнститут биологических проблем Севера, ДВО РАНРоссияInstitute of Biological Problems of the North, Far Eastern Branch of the Russian Academy of SciencesRussian Federation202007112020754285290Copyright © Скоробрехова Е.М., 20202020Скоробрехова Е.М.Skorobrekhova E.M.Данная работа распространяется под лицензией Creative Commons Attribution 4.0.This work is licensed under a Creative Commons Attribution 4.0 License.https://vestnik-bio-msu.elpub.ru/jour/article/view/940

С использованием методов световой и трансмиссионной электронной микроскопии изучено строение капсулы и цисты, окружающих метацеркарии трематод Podocotyle atomon во вторых промежуточных хозяевах – Locustogammarus locustoides (Brandt, 1851) и Spinulogammarus ochotensis (Brandt, 1851). Показано, что образованная тканью хозяина капсула состоит из двух слоев: 1) электронно-светлого, рыхловатого, внутреннего и 2) электронно-темного, деструктурированного наружного слоя, включающего небольшое количество клеток и их фрагментов. Циста, образуемая самим паразитом, организована в виде единого аморфного слоя. Предполагается, что внутренний слой капсулы подокотилей образуется путем дегенерации и трансформации участка мышечного волокна амфиподы, в котором локализуется личинка, а ее наружный слой представлен отложениями пигмента.

We assessed the structure of Podocotyle atomon metacercariae cysts and their surrounding capsules in the trematodeʼs second intermediate hosts – Locustogammarus locustoides (Brandt, 1851) and Spinulogammarus ochotensis (Brandt, 1851) – using light and transmission electron microscopy. The cysts, which are synthesized from materials secreted by the parasite tegument, exhibited a single, amorphous layer. The capsules formed by the host consisted of two layers: an electron-light, loose inner layer and an electron-dark, unstructured outer layer, which incorporated a small amount of intact and fragmented host cells. The inner capsule layer may have been formed by degeneration and transformation of the amphipodʼs muscle tissue that surrounds the larva. The outer layer of the capsule appeared to contain pigment deposits.

ультраструктураамфиподыметацеркариицистакапсулаPodocotyle atomonultrastructureamphipodsmetacercariaecystcapsulePodocotyle atomonРабота выполнена в рамках государственного задания «Таксономическое, морфологическое и экологическое разнообразие гельминтов позвоночных животных Северной Азии» № ААА-А17-117012710031-6.ReferencesОрловская О.М. Новые сведения о жизненных циклах некоторых видов трематод прибрежных рыб Северного Охотоморья // Биоразнообразие и экология паразитов. Труды Центра паразитологии. Том XLVI / Под ред. С.В. Зиновьева. М.: Наука, 2010. C. 186–197.Орловская О.М. Новые сведения о жизненных циклах некоторых видов трематод прибрежных рыб Северного Охотоморья // Биоразнообразие и экология паразитов. Труды Центра паразитологии. Том XLVI / Под ред. С.В. Зиновьева. М.: Наука, 2010. C. 186–197.Успенская А.В. Паразитофауна бентических ракообразных Баренцева моря. М.: Изд-во акад. наук СССР, 1963. 126 с.Успенская А.В. Паразитофауна бентических ракообразных Баренцева моря. М.: Изд-во акад. наук СССР, 1963. 126 с.James B.L. Host selection and ecology of marine digenean larvae // Fourth European marine biology symposium / Ed. D.J Crisp. Cambridge: Cambridge Univ. Press, 1971. P. 179–197.James B.L. Host selection and ecology of marine digenean larvae // Fourth European marine biology symposium / Ed. D.J Crisp. Cambridge: Cambridge Univ. Press, 1971. P. 179–197.Køie M. On the morphology and life history of Podocotyle reflexa and a comparison of its developmental stages with those of P. atomon (Trematoda, Opecoelidae) // Ophelia. 1980. Vol. 20. N 1. P. 17–43.Køie M. On the morphology and life history of Podocotyle reflexa and a comparison of its developmental stages with those of P. atomon (Trematoda, Opecoelidae) // Ophelia. 1980. Vol. 20. N 1. P. 17–43.Shimazu T. On two metacercariae on the genus Podocotyle from the shrimp, Pandalus goniurus, from Aniwa Bay, Sakhalin, USSR (Trematoda, Opecoelidae) // Bull. Japan. Soc. Sci. Fish. 1973. Vol. 39. N 5. P. 481–487.Shimazu T. On two metacercariae on the genus Podocotyle from the shrimp, Pandalus goniurus, from Aniwa Bay, Sakhalin, USSR (Trematoda, Opecoelidae) // Bull. Japan. Soc. Sci. Fish. 1973. Vol. 39. N 5. P. 481–487.Lo S.J., Hall J.E., Allender P.A., Klainer A.S. Scanning electron microscopy of an opecoelid cercaria and its encystment and encapsulation in an insect host // J. Parasitol. 1975. Vol. 61. N 3. P. 413–417.Lo S.J., Hall J.E., Allender P.A., Klainer A.S. Scanning electron microscopy of an opecoelid cercaria and its encystment and encapsulation in an insect host // J. Parasitol. 1975. Vol. 61. N 3. P. 413–417.Knowles E.E., Hall J.E. Histopathology of an opecoelid trematode infection in mayfly Naiads // J. Invert. Pathol. 1976. Vol. 27. N 3. P. 351–362.Knowles E.E., Hall J.E. Histopathology of an opecoelid trematode infection in mayfly Naiads // J. Invert. Pathol. 1976. Vol. 27. N 3. P. 351–362.Beckett E.B. Histological changes in mosquito flight muscle fibres associated with parasitization by filarial larvae // Parasitology. 1971. Vol. 63. N 3. P. 365–372.Beckett E.B. Histological changes in mosquito flight muscle fibres associated with parasitization by filarial larvae // Parasitology. 1971. Vol. 63. N 3. P. 365–372.Chikilian M.L., Bradley T.J., Nayar J.K., Knight J.W. Ultrastructural comparison of extracellular and intracellular encapsulation of Brugia malayi in Anopheles quadrimaculatus // J. Parasitol. 1994. Vol. 80. N 1. P. 133–140.Chikilian M.L., Bradley T.J., Nayar J.K., Knight J.W. Ultrastructural comparison of extracellular and intracellular encapsulation of Brugia malayi in Anopheles quadrimaculatus // J. Parasitol. 1994. Vol. 80. N 1. P. 133–140.Глупов В.В., Слепнева И.А., Дубовский И.М. Генерация активированных кислородных метаболитов при формировании иммунного ответа у членистоногих // Труды Зоол. ин-та. РАН. 2009. Т. 313. № 3. С. 297–307.Глупов В.В., Слепнева И.А., Дубовский И.М. Генерация активированных кислородных метаболитов при формировании иммунного ответа у членистоногих // Труды Зоол. ин-та. РАН. 2009. Т. 313. № 3. С. 297–307.Dubovskiy I.M., Kryukova N.A., Glupov V.V., Ratcliffe N.A. Encapsulation and nodulation in insects // Invert. Surv. J. 2016. Vol. 13. N 1. P. 229–246.Dubovskiy I.M., Kryukova N.A., Glupov V.V., Ratcliffe N.A. Encapsulation and nodulation in insects // Invert. Surv. J. 2016. Vol. 13. N 1. P. 229–246.Chen C.C. Further evidence of both humoral and cellular encapsulations of sheathed microfilariae of Brugia pahangi in Anopheles quadrimaculatus // Parasitology. 1988. Vol. 78. N 6. P. 819–826.Chen C.C. Further evidence of both humoral and cellular encapsulations of sheathed microfilariae of Brugia pahangi in Anopheles quadrimaculatus // Parasitology. 1988. Vol. 78. N 6. P. 819–826.Nappi A., Poirié M., Carton Y. The role of melanization and cytotoxic by products in the cellular immune responses of Drosophila against parasitic wasps // Adv. Parasitol. 2009. Vol. 70. N 4. P. 99–121.Nappi A., Poirié M., Carton Y. The role of melanization and cytotoxic by products in the cellular immune responses of Drosophila against parasitic wasps // Adv. Parasitol. 2009. Vol. 70. N 4. P. 99–121.Kim-Jo C., Gatti J-L., Poirié M. Drosophila cellular immunity against parasitoid wasps: a complex and timedependent process // Frontiers Physiol. 2019. Vol. 10: 603.Kim-Jo C., Gatti J-L., Poirié M. Drosophila cellular immunity against parasitoid wasps: a complex and timedependent process // Frontiers Physiol. 2019. Vol. 10: 603.Dezfuli B.S., Simoni E., Duclos L., Rossetti E. Crustacean-acanthocephalan interaction and host cellmediated immunity: parasite encapsulation and melanization // Folia Parasitol. 2008. Vol. 55. N 1. P. 53–59.Dezfuli B.S., Simoni E., Duclos L., Rossetti E. Crustacean-acanthocephalan interaction and host cellmediated immunity: parasite encapsulation and melanization // Folia Parasitol. 2008. Vol. 55. N 1. P. 53–59.Kostadinova A., Mavrodieva R.S. Microphallids in Gammarus insensibilis Stock, 1966 from a Black Sea Lagoon: host response to infection // Parasitology. 2005. Vol. 131. N 3. P. 347–354.Kostadinova A., Mavrodieva R.S. Microphallids in Gammarus insensibilis Stock, 1966 from a Black Sea Lagoon: host response to infection // Parasitology. 2005. Vol. 131. N 3. P. 347–354.Skorobrekhova E.M., Nikishin V.P. Encapsulation of the acanthocephalan Corynosoma strumosum (Rudolphi, 1802) Lühe, 1904, in the intermediate host Spinulogammarus ochotensis // J. Parasitol. 2019. Vol. 105. N 4. P. 567–570.Skorobrekhova E.M., Nikishin V.P. Encapsulation of the acanthocephalan Corynosoma strumosum (Rudolphi, 1802) Lühe, 1904, in the intermediate host Spinulogammarus ochotensis // J. Parasitol. 2019. Vol. 105. N 4. P. 567–570.Christensen B.M., Li J., Chen C.-C., Nappi A.J. Melanization immune responses in mosquito vectors // Trends Parasitol. 2005. Vol. 21. N 4. P. 192–199.Christensen B.M., Li J., Chen C.-C., Nappi A.J. Melanization immune responses in mosquito vectors // Trends Parasitol. 2005. Vol. 21. N 4. P. 192–199.Carton Y., Frey F., Nappi A.J. Inheritance of cellular immune resistance in Drosophila melanogaster // Heredity. 1992. Vol. 69. P. 393–399.Carton Y., Frey F., Nappi A.J. Inheritance of cellular immune resistance in Drosophila melanogaster // Heredity. 1992. Vol. 69. P. 393–399.Salt G. The cellular defense reactions of insects. Cambridge monograph in experimental biology. London: Cambridge Univ. Press, 1970. 118 pp.Salt G. The cellular defense reactions of insects. Cambridge monograph in experimental biology. London: Cambridge Univ. Press, 1970. 118 pp.Kobayashi M., Ogura N., Yamamoto H. Studies on filariasis VIII: histological observation on the abortive development of Brugia malayi larvae in the thoracic muscles of the mosquitoes, Armigeres subalbatus // Jpn. J. Sanit. Zool. 1986. Vol. 37. N 2. P. 127–132.Kobayashi M., Ogura N., Yamamoto H. Studies on filariasis VIII: histological observation on the abortive development of Brugia malayi larvae in the thoracic muscles of the mosquitoes, Armigeres subalbatus // Jpn. J. Sanit. Zool. 1986. Vol. 37. N 2. P. 127–132.Dedkhad W., Christensen B.M., Bartholomay L.C., Joshi D., Hempolchom C., Saeung A. Immune responses of Aedes togoi, Anopheles paraliae and Anopheles lesteri against nocturnally subperiodic Brugia malayi microfilariae during migration from the midgut to the site of development // Parasites Vectors. 2018. Vol. 11: 528.Dedkhad W., Christensen B.M., Bartholomay L.C., Joshi D., Hempolchom C., Saeung A. Immune responses of Aedes togoi, Anopheles paraliae and Anopheles lesteri against nocturnally subperiodic Brugia malayi microfilariae during migration from the midgut to the site of development // Parasites Vectors. 2018. Vol. 11: 528.Martin T.R., Conn D.B. The pathogenicity, localization, and cyst structure of echinostomatid metacercariae (Trematoda) infecting the kidneys of the frogs Rana clamitans and Rana pipiens // J. Parasitol. 1990. Vol. 76. N 3. P. 414–419.Martin T.R., Conn D.B. The pathogenicity, localization, and cyst structure of echinostomatid metacercariae (Trematoda) infecting the kidneys of the frogs Rana clamitans and Rana pipiens // J. Parasitol. 1990. Vol. 76. N 3. P. 414–419.Taft S.J. Cinephotomicrographic and histochemical observations on cercarial penetration and encystment by Plagiorchis sp. in larvae of Chaoborus sp. // Trans. Am. Micros. Soc. 1990. Vol. 109. N 2. P. 160–167.Taft S.J. Cinephotomicrographic and histochemical observations on cercarial penetration and encystment by Plagiorchis sp. in larvae of Chaoborus sp. // Trans. Am. Micros. Soc. 1990. Vol. 109. N 2. P. 160–167.Galaktionov K.V., Malkova I.I., Irwin S.W.B., Saville D.H., Maguire J.G. The structure and formation of metacercarial cysts in the trematode family Microphallidae Travassos 1920 // J. Helminthol. 1997. Vol. 71. N 1. P. 13–20.Galaktionov K.V., Malkova I.I., Irwin S.W.B., Saville D.H., Maguire J.G. The structure and formation of metacercarial cysts in the trematode family Microphallidae Travassos 1920 // J. Helminthol. 1997. Vol. 71. N 1. P. 13–20.

The authors declare that there are no conflicts of interest present.