Role of reactive oxygen species in tone regulation of respiratory and locomotor muscle feed arteries in the rat

The skeletal muscles contractile activity is associated with increased reactive oxygen species (ROS) production. Respiratory and locomotor muscles differ in the patterns of contractile activity, which can create different conditions for ROS production and their effect on feed arteries. The aim of this work was to study the role of ROS, including produced by NADPH oxidases (NOX), in diaphragm artery (a. phrenica) and deep shoulder artery (a. profunda brachii) tone regulation of the rat. Vasomotor responses of arterial preparations were studied in isometric regimen. The NOX inhibitor, VAS2870, caused relaxation, which was more pronounced in the diaphragm arteries compared to the shoulder arteries: at the concentration of 1 μM, the diaphragm arteries relaxed to 33%, and the shoulder arteries – to 91% of the precontraction level. Tiron (O2. - scavenger) showed similar results: at the 10 mM concentration, it caused relaxation of the diaphragm arteries to 38%, and the shoulder arteries – to 66%. At the same time, catalase (3000 U/ml) increased the deep shoulder arteries contraction but did not affect it in the diaphragm arteries. Using quantitative PCR, it was shown that the contents of mRNA isoforms NOX, p22phox, p47phox, p67phox, Poldip2, Gpx-1, SOD-1 and catalase do not differ between arteries, while the content of SOD-3 mRNA in the diaphragm arteries is less than in the shoulder arteries. Thus, the contribution of ROS, produced by NOX, to the feed arteries tone regulation of the respiratory muscles is higher than in the locomotor muscles. Experiments using tiron and catalase have shown that O2. - increases the arteries contractile responses, while H2O2, on the contrary, causes the shoulder arteries relaxation. One of the reasons for the more pronounced effect of O2 - in the diaphragm arteries may be the relatively low SOD-3 expression.

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