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USING THE DYNAMIC PHASE MICROSCOPY FOR NEURON CYTOPLASM STUDIES

https://doi.org/10.1234/XXXX-XXXX-2014-4-25-31

Abstract

Using laser interference microscopy (LIM) an approach to study the state of the plasma membrane (PM) and cytoplasmic-membrane region neuron (vertebrates and invertebrates) was developed. The changes of the PM and neurons cytoplasm state at rest and during the modification of the functioning (a neurotransmitter, acetylcholine) was studied. The method for evaluation of the kinetics of changes in PM and neurons cytoplasm in different parts of the cell was elaborate. Regular PM and neurons cytoplasm processes, the frequency of which depends on the functional state of the neurons was found. It was suggested that the fluctuations depend on the PM relief changes, the restructuring of cytoskeleton-membrane and movement of intracellular vesicles in neurons cytoplasm.

About the Authors

T. A. Kazakova

Russian Federation


S. M. Novikov

Russian Federation


G. G. Levin
Всероссийский НИИ оптико-физических измерений
Russian Federation


G. V. Maksimov

Russian Federation


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Review

For citations:


Kazakova T.A., Novikov S.M., Levin G.G., Maksimov G.V. USING THE DYNAMIC PHASE MICROSCOPY FOR NEURON CYTOPLASM STUDIES. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2014;(4):25-31. (In Russ.) https://doi.org/10.1234/XXXX-XXXX-2014-4-25-31

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ISSN 0137-0952 (Print)