Influence of indole on intracellular polyamines and antibiotic susceptibility of Escherichia coli
Abstract
Small regulatory molecules such as indole and polyamines are involved in the regulation of various processes in bacteria, including the response to antibiotics. It is known that small regulatory molecules can influence each other, but there is no information on the interaction of indole and polyamines. We have shown here that indole at micromolar concentrations reduces the susceptibility of Escherichia coli to fluoroquinolone, beta-lactam and aminoglycoside antibiotics. Indole had a stronger effect on the antibiotic susceptibility of bacteria capable of synthesizing the polyamines putrescine and spermidine as compared to a polyamine-deficient isogenic strain. Exogenous indole increased intracellular level of putrescine and spermidine by 2 and 2.5 folds, respectively, and had no effect on the cadaverine level. The supplementation of cultivation media with common bacterial polyamines, putrescine, cadaverine, and spermidine, did not influence the production of indole by E. coli cells. The addition of spermine, a polyamine mainly synthesized by eukaryotes, increased the release of indole into the medium by E. coli cells (by no more than 20%). Thus, indole reduces antibiotic susceptibility of E. coli to antibiotics with different mechanisms of antibacterial action, in particular, by increasing the amount of polyamines in bacterial cells.
About the Authors
L. Y. Nesterova
Институт экологии и генетики микроорганизмов УрО РАН, Пермский федеральный исследовательский центр УрО РАН
Russian Federation
Russian Federation
A. V. Akhova
Институт экологии и генетики микроорганизмов УрО РАН, Пермский федеральный исследовательский центр УрО РАН
Russian Federation
Russian Federation
A. G. Tkachenko
Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Center, Ural Brunch of the Russian Academy of Sciences
Russian Federation
Russian Federation
Golev st. 13, Perm, 614081
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Review
For citations:
Nesterova L.Y., Akhova A.V., Tkachenko A.G. Influence of indole on intracellular polyamines and antibiotic susceptibility of Escherichia coli. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2021;76(4):219-224. (In Russ.)
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