Morphofunctional characteristics of fibroblasts of the papillary and reticular layers of the dermis of human skin
Abstract
Fibroblasts of the dermis are a heterogeneous population – among them there are several subpopulations that differ in origin, anatomical regional specificity and location in the thickness of the dermis. We investigated some phenotypic and functional features of fibroblasts of the papillary and reticular dermis of human skin. It was found that the introduction of reticular fibroblasts into the primary culture with an oxygen level reduced to 5% increases the efficiency of obtaining this population of fibroblasts. Morphometric analysis of cultured fibroblasts of the studied populations, comparison of the strength and rate of collagen gel contraction, and data on the expression levels of CD90 and CD73 obtained by flow cytometry showed significant differences in fibroblasts of the papillary and reticular layers of the human dermis of the temporal region.
Keywords
human skin,
dermis,
cultivation of cells,
fibroblasts,
reticular fibroblasts,
papillary fibroblasts,
collagen gel contraction
Supporting agencies: The research was funded by Russian Science Foundation, project number № 21-74-30015.
About the Authors
O. S. Rogovaya
N.K. Koltsov Institute of Developmental Biology, Russian Academy of Sciences
Russian Federation
Russian Federation
Vavilova 26 Moscow, 119334
A. O. Zupnik
Lomonosov Moscow State University, Leninskiye gory 1–12, Moscow
Russian Federation
Russian Federation
Department of Cell Biology and Histology, Faculty of Biology
L. Sh. Izmailova
N.K. Koltsov Institute of Developmental Biology, Russian Academy of Sciences
Russian Federation
Russian Federation
Vavilova 26 Moscow, 119334
E. A. Vorotelyak
N.K. Koltsov Institute of Developmental Biology, Russian Academy of Sciences
Russian Federation
Russian Federation
Vavilova 26 Moscow, 119334
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Review
For citations:
Rogovaya O.S., Zupnik A.O., Izmailova L.Sh., Vorotelyak E.A. Morphofunctional characteristics of fibroblasts of the papillary and reticular layers of the dermis of human skin. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2021;76(4):250-257. (In Russ.)
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