PILOT STUDY OF A POTENTIAL GEROPROTECTOR, “QUINTON MARINE PLASMA”, IN EXPERIMENTS ON CULTURED CELLS
https://doi.org/10.1234/XXXX-XXXX-2015-1-9-13
Abstract
Effect of isotonic “Quinton Marine Plasma” (QMP) solution on the growth and “stationary phase aging” (accumulation of “age” changes in cultured cells during cell proliferation slowing down within a single passage and subsequent “aging” in the stationary phase of growth) of transformed Chinese hamster cells was studied. No positive effects of QMP on the studied viability indexes of the cultured cells were found in all the experiments. It is assumed that QMP, like many other potential anti-aging agents the authors studied recently (2,4-dinitrophenol in concentrations that provide mild uncoupling, the essential oil of oregano, hydrated C60-fullerene, etc.), can demonstrate its beneficial effect only at the level of the whole organism, triggering neurohumoral mechanisms that are not present in cytological model systems.
About the Authors
A. N. KhokhlovRussian Federation
G. V. Morgunova
Russian Federation
T. S. Ryndina
Russian Federation
F. Coll
Russian Federation
References
1. Yoshizawa Y., Tanojo H., Kim S.J., Maibach H.I. Sea water or its components alter experimental irritant dermatitis in man // Skin Res. Technol. 2001. Vol. 7. P. 36—39.
2. Kimata H., Tai H., Nakagawa K., Yokoyama Y., Nakajima H., Ikegami Y. Improvement of skin symptoms and mineral imbalance by drinking deep sea water in patients with atopic eczema/dermatitis syndrome (AEDS) // Acta Medica (Hradec Kralove). 2002. Vol. 45. N 2. P. 83—84.
3. Yoshioka S., Hamada A., Cui T., Yokota J., Yamamoto S., Kusunose M., Miyamura M., Kyotani S., Kaneda R., Tsutsui Y., Odani K., Odani I., Nishioka Y. Pharmacological activity of deep-sea water: examination of hyperlipemia prevention and medical treatment effect // Biol. Pharm. Bull. 2003. Vol. 26. N 11. P. 1552—1559.
4. Tabary O., Muselet C., Miesch M.C., Yvin J.C., Clйment A., Jacquot J. Reduction of chemokine IL-8 and RANTES expression in human bronchial epithelial cells by a sea-water derived saline through inhibited nuclear factor-kB activation // Biochem. Biophys. Res. Commun. 2003. Vol. 309. N 2. P. 310—316.
5. Miyamura M., Yoshioka S., Hamada A., Takuma D., Yokota J., Kusunose M., Kyotani S., Kawakita H., Odani K., Tsutsui Y., Nishioka Y. Difference between deep seawater and surface seawater in the preventive effect of atherosclerosis // Biol. Pharm. Bull. 2004. Vol. 27. N 11. P. 1784—1787.
6. Hataguchi Y., Tai H., Nakajima H., Kimata H. Drinking deep-sea water restores mineral imbalance in atopic eczema/dermatitis syndrome // Eur. J. Clin. Nutr. 2005. Vol. 59. P. 1093—1096.
7. Slapak I., Skoupб J., Strnad P., HornнkP.Efficacy of isotonic nasal wash (seawater) in the treatment and prevention of rhinitis in children // Arch. Otolaryngol. Head Neck Surg. 2008. Vol. 134. N 1. P. 67—74.
8. Original Quinton [Электронный ресурс]. 2010. (URL: http://www.originalquinton.com/ (дата обращения: 04.09.2014).
9. Alberola J., Coll F. Marine therapy and its healing properties // Curr. Aging Sci. 2013. Vol. 6. N 1. P. 63—75.
10. Martнnez-Peinado P., Maseres-Javaloy P., Martнnez-Lуpez J.E., Garcнa-Irles M., Sempere-Ortells J.M. In vitro and in vivo studies to evaluate a potential activity of Quinton Solution on the immune system // International Immunology Meeting Abstracts (Suppl. 1. Pt. 3). Oxford: Oxford University Press, 2010. P. iii93.
11. Хохлов А.Н. Пролиферация и старение // Итоги науки и техники ВИНИТИ АН СССР. Сер. Общие проблемы физико-химической биологии. Т. 9. М.: ВИНИТИ, 1988. 176 с.
12. Khokhlov A.N. Stationary cell cultures as a tool for gerontological studies // Ann. N.Y. Acad. Sci. 1992. Vol. 663. P. 475—476.
13. Khokhlov A.N. Cell proliferation restriction: is it the primary cause of aging? // Ann. N.Y. Acad. Sci. 1998. Vol. 854. P. 519.
14. Khokhlov A.N. Does aging need its own program, or is the program of development quite sufficient for it? Stationary cell cultures as a tool to search for anti-aging factors // Curr. Aging Sci. 2013. Vol. 6. N 1. P. 14—20.
15. Khokhlov A.N. Decline in regeneration during aging: appropriateness or stochastics? // Russ. J. Dev. Biol. 2013. Vol. 44 . N 6. P. 336—341.
16. Khokhlov A.N. Impairment of regeneration in aging: appropriateness or stochastics? // Biogerontology. 2013. Vol. 14. N 6. P. 703—708.
17. Khokhlov A.N. Evolution of the term “cellular senescence” and its impact on the current cytogerontological research // Moscow Univ. Biol. Sci. Bull. 2013. Vol. 68. N 4. P. 158—161.
18. Khokhlov A.N. What will happen to molecular and cellular biomarkers of aging in case its program is canceled (provided such a program does exist)? // Adv. Gerontol. 2014. Vol. 4. N 2. P. 150—154.
19. Khokhlov A.N., Klebanov A.A., Karmushakov A.F., Shilovsky G.A., Nasonov M.M., Morgunova G.V. Testing of geroprotectors in experiments on cell cultures: choosing the correct model system // Moscow Univ. Biol. Sci. Bull. 2014. Vol. 69. N 1. P. 10—14.
20. Khokhlov A.N. From Carrel to Hayflick and back, or what we got from the 100-year cytogerontological studies // Biophysics. 2010. Vol. 55. N 5. P. 859—864.
21. Alinkina E.S., Vorobyova A.K., Misharina T.A., Fatkullina L.D., Burlakova E.B., Khokhlov A.N. Cytogerontological studies of biological activity of oregano essential oil // Moscow Univ. Biol. Sci. Bull. 2012. Vol. 67. N 2. P. 52—57.
22. Yablonskaya O.I., Ryndina T.S., Voeikov V.L., Khokhlov A.N. A paradoxical effect of hydrated C60-fullerene at an ultralow concentration on the viability and aging of cultured Chinese hamster cells // Moscow Univ. Biol. Sci. Bull. 2013. Vol. 68. N 2. P. 63—68.
23. Khokhlov A.N. Can cancer cells age? Stationary cell culture approach to the problem solution // Visualizing of senescent cells in vitro and in vivo. Programme and abstracts (Warsaw, Poland, 15—16 December 2012). Warsaw, 2012. P. 48—49.
24. Lewis M.R. Sea water as a medium for tissue cultures // Anat. Rec. 1916. Vol. 10. N 4. P. 287—299.
25. Berges J.A, Franklin D.J., Harrison P.J. Evolution of an artificial seawater medium: improvements in enriched seawater, artificial water over the last two decades // J. Phycol. 2001. Vol. 37. N 6. P. 1138—1145.
26. Khokhlov A.N., Wei L., Li Y., He J. Teaching cytogerontology in Russia and China // Adv. Gerontol. 2012. Vol. 25. N 3. P. 513—516.
27. Wei L., Li Y., He J., Khokhlov A.N. Teaching the cell biology of aging at the Harbin Institute of Technology and Moscow State University // Moscow Univ. Biol. Sci. Bull. 2012. Vol. 67. N 1. P. 13—16.
28. Khokhlov A.N. The cell kinetics model for determination of organism biological age and for geroprotectors or geropromoters studies // Biomarkers of aging: expression and regulation. Proceeding / Eds. F. Licastro, C.M. Caldarera. Bologna: CLUEB, 1992. P. 209—216.
29. Moldaver M.V., Yegorov Y.E. Sparse plating increases the heterogeneity of proliferative potential of fibroblasts // Mech. Ageing Dev. 2009. Vol. 130. N 5. P. 337—342.
30. Бурнаевский Н.С., Вишнякова Х.С., Сафенина А.В., Рыбалко Д.В., Попов К.В., Егоров Е.Е. Влияние парциального давления кислорода на эффективность колониеобразования и дифференцировки мезенхимальных стромальных клеток человека, полученных из различных источников // Клеточная трансплантология и тканевая инженерия. 2010. Т. 5. № 4. С. 24—30.
Review
For citations:
Khokhlov A.N., Morgunova G.V., Ryndina T.S., Coll F. PILOT STUDY OF A POTENTIAL GEROPROTECTOR, “QUINTON MARINE PLASMA”, IN EXPERIMENTS ON CULTURED CELLS. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2015;(1):9-13. (In Russ.) https://doi.org/10.1234/XXXX-XXXX-2015-1-9-13