The role of protein kinase C isoforms in the formation of neutrophil extracellular traps
Abstract
Neutrophils release decondensed nuclear chromatin or neutrophil extracellular traps (NET) in response to a great number of physiological and pharmacological stimuli. However, apart from the host defensive function, NETs play an essential role in the pathogenesis of various autoimmune, inflammatory, and malignant diseases. Therefore, understanding the molecular mechanisms of NET formation, usually leading to the neutrophil death (NETosis), is important to control the consequences of aberrant or excessive NET release. Protein kinase C (PKC) is a serine/threonine kinase that is involved in a variety of neutrophil functions, but its role in NETosis is not well understood. Since five PKC isoforms (α, βI, βII, δ, and ζ) have been described in human neutrophils, we studied their contribution to NETosis and oxidative burst using inhibitory analysis. Using specific PKC isoform inhibitors, we have shown that PKCβ, PKCδ, and PKCζ are involved in the oxidative burst and NETosis activated by calcium ionophore A23187, while PKCβ is involved in the oxidative burst and NETosis upon cell activation by diacylglycerol mimetic phorbol 12-myristate 13-acetate.
Keywords
Supporting agencies: The research was carried out within the framework of the project “Molecular and cellular bases of immunity” (project 21-1-21, CITIS no. 121042600047-9).
About the Authors
N. V. Vorobjeva
Department of Immunology, Biology Faculty, Lomonosov Moscow State University
Russian Federation
Russian Federation
1–12 Leninskie Gory, Moscow, 119234, Russia
S. S. Vakhlyarskaya
Russian Children’s Clinical Hospital, N.I. Pirogov Russian National Research Medical University
Russian Federation
Russian Federation
117 Leninsky prospect, Moscow, 119571, Russia
B. V. Chernyak
A.N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University
Russian Federation
Russian Federation
1–40 Leninskie Gory, Moscow, 119992, Russia
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Review
For citations:
Vorobjeva N.V., Vakhlyarskaya S.S., Chernyak B.V. The role of protein kinase C isoforms in the formation of neutrophil extracellular traps. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2022;77(2):112–121. (In Russ.)
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