Evaluation of the spectrum of proteolytic activity of micromycetes of the genus Aspergillus in relation to proteins of the hemostasis system
Abstract
The activity of extracellular proteinases in seven strains of different species of micromycetes of the genus Aspergillus in relation to proteins of the hemostasis system was studied. Comparison of the values of enzymatic indices during the growth of strains on agar media with casein and fibrin, followed by their submerged cultivation and analysis of amidolytic activity with chromogenic peptide substrates of proteinases of the hemostasis system, made it possible to select strains of A. candidus A4 and A. crustosus A29 as promising producers of fibrinolytic proteinases. The proteinases formed by both strains were able to cleave the chromogenic peptide substrates of thrombin, plasmin, factor X, protein C, and urokinase, exhibiting high plasmin-like and thrombin-like activity and not having an activating effect on the proenzymes of the hemostasis system.
Keywords
micromycete proteinases,
fibrinolytic enzymes,
thrombolytic agents,
plasmin-like activity,
hemostasis protein activators,
chromogenic peptide substrates
Supporting agencies: The work was supported by the Financial Council for Grants of the President of the Russian Federation No. SP-3906.2021.4.
About the Authors
A. A. Osmolovsky
Department of Microbiology, Faculty of Biology, Lomonosov Moscow State University
Russian Federation
Russian Federation
1–12 Leninskie Gory, Moscow, 119234, Russia
B. Şaş
Department of Molecular Biology and Genetics, Faculty of Natural Sciences and Literature, Istanbul Kültür University
Turkey
Turkey
Ataköy 7-8-9-10, E5 Karayolu Üzeri Ataköy Yerleşkesi, Bakırköy, 34158, Istanbul, Turkey
A. V. Aleksandrova
Department of Mycology and Algology, Faculty of Biology, Lomonosov Moscow State University
Russian Federation
Russian Federation
1–12 Leninskie Gory, Moscow, 119234, Russia
N. A. Baranova
Department of Microbiology, Faculty of Biology, Lomonosov Moscow State University
Russian Federation
Russian Federation
1–12 Leninskie Gory, Moscow, 119234, Russia
V. G. Kreyer
Department of Microbiology, Faculty of Biology, Lomonosov Moscow State University
Russian Federation
Russian Federation
1–12 Leninskie Gory, Moscow, 119234, Russia
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Review
For citations:
Osmolovsky A.A., Şaş B., Aleksandrova A.V., Baranova N.A., Kreyer V.G. Evaluation of the spectrum of proteolytic activity of micromycetes of the genus Aspergillus in relation to proteins of the hemostasis system. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2022;77(2):138–144. (In Russ.)
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