DEPENDENCE OF GROWTH CHARACTERISTICS OF NATURAL STRAINS OF LACTOCOCCUS LACTIS SUBSP. LACTIS ON THE COMPOSITION OF THE NUTRIENT AGAR MEDIA USED FOR BIOMASS GROWTH
https://doi.org/10.1234/XXXX-XXXX-2015-1-31-36
Abstract
The results of dependence of growth characteristics of strains Lactococcus lactis subsp. lactis 729 and TV2 isolated from milk and homemade curd the content in the agar medium peptone from casein as a main nitrogen source. Different composition on the level of enrichment of the agar medium was used for biomass growth strains in the study of growth characteristics in liquid media with the same composition. Compared the number of colony forming units (CFU), the rate of cell division (TDC) and the average hourly doubling time (AHDT) at two time points of cultivation: from 1,0 to 2,5 and from 2,5 to 3,5 hours. Liquid culture medium was maintained logarithmic growth of strain 729 in all variations on a time interval of cultivation to 3,5 hours with a maximum decrease in pH to 6,5. Maximum accumulation level of biomass (2,5 Ѕ 108 cells/ml) was observed when cultured in a pair of media containing meat extract, and the smallest AHDT (37,5 min) when moving biomass MA4 minimal medium (peptone from casein, and 0,4% lactose 0,4%) and rich in containing yeast extract liquid medium M7. Strain 729 in contrast to a strain incapable TB2 form colonies when the content of casein peptone agar medium is less than 0,4%. Reducing the amount of nitrogen source (peptone from casein from 1,0% to 0,4%) resulted in an increase in colony size strain TV2 from
2,85 mm2 to5mm2. The stimulatory effect of yeast extract in liquid media under these concentration ratios allowing cell division to increase the effectiveness while reducing the accumulation of lactic acid to achieve critical acidification (pH 5,5) blocks the transport of oligopeptides into a cell. Meat extract contributed bacteria adapt to the conditions of acidity and has a positive effect on cell division of strain 729 at a late stage of development of bacterial populations. Comparative small size of the colonies (area ~0,5 mm2 , diameter ~0,8 mm) can be a sign of strain as active acidifier.
About the Authors
M. A. Treninа
Лаборатория иммунологии ФГБУ НИИ вирусологии им. Д.И. Ивановского
Russian Federation
Russian Federation
H. S. Epremyan
Научно-производственный центр “Армбиотехнология” НАН РА государственная некоммерческая организация (НПЦ “Армбио-технология” НАН РА, ГНКО)
Russian Federation
Russian Federation
L. G. Stoyanova
Russian Federation
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For citations:
Treninа M.A., Epremyan H.S., Stoyanova L.G. DEPENDENCE OF GROWTH CHARACTERISTICS OF NATURAL STRAINS OF LACTOCOCCUS LACTIS SUBSP. LACTIS ON THE COMPOSITION OF THE NUTRIENT AGAR MEDIA USED FOR BIOMASS GROWTH. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2015;(1):31-36. (In Russ.) https://doi.org/10.1234/XXXX-XXXX-2015-1-31-36
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