Aspergillus proteolytic enzymes hydrolyzing fibrillar proteins for biomedicine and biotechnological process
Abstract
Fibrillar proteins play a significant role in the living organisms functioning, and recently much attention has been paid to them as targets in the therapy and diagnosis of various diseases, substrates for the biopolymers production, as well as industrially important proteins that are key agents in the textile and food manufacture. To implement the development of these directions, fibrillar proteins hydrolysis and processing methods are needed, the most promising of which is the use of proteolytic enzymes. 11 enzyme preparations obtained by cultivation of micromycetes belong to the genus Aspergillus and containing from 1 to 10 proteins were studied for the ability to hydrolyze fibrillar proteins: collagen, keratin, fibrin and elastin. The proteolytic enzymes of A. alliaceus 7dN1, A. clavatus A16, and A. ustus 1 showed an activity comparable to or higher than that of the commercial preparation Terrilitin used in wound therapy. The A. fumigatus D1 enzyme preparation also showed high level of fibrinolytic activity.
Keywords
proteases,
fibrillar proteins,
collagenases,
keratinases,
fibrinolytic enzymes,
elastases,
Aspergillus
Supporting agencies: The research was funded by Russian Science Foundation, project number 22-24-00674. The studies were performed without the use of animals and without involving people as subjects. The authors declare no conflict of interest.
About the Authors
S. N. Timorshina
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Microbiology, Faculty of Biology
1–12 Leninskie gory, Moscow, 119234
E. A. Popova
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Microbiology, Faculty of Biology
1–12 Leninskie gory, Moscow, 119234
A. A. Galiakberova
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Microbiology, Faculty of Biology
1–12 Leninskie gory, Moscow, 119234
A. G. Ochneva
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Microbiology, Faculty of Biology
1–12 Leninskie gory, Moscow, 119234
A. A. Osmolovskiy
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Microbiology, Faculty of Biology
1–12 Leninskie gory, Moscow, 119234
References
1. Yeo J., Jung G., Tarakanova A., Martín-Martínez F.J., Qin Z., Cheng Y., Zhang Y.W., Buehler M.J. Multiscale modeling of keratin, collagen, elastin and related human diseases: Perspectives from atomistic to coarse-grained molecular dynamics simulations // Extreme Mech. Lett. 2021. Vol. 20. P. 112–124.
2. Sweeney S.M., Orgel J.P., Fertala A., McAuliffe J.D., Turner K.R., Di Lullo G.A., Barnes A.M., Marini J.C., San Antonio J.D. Candidate cell and matrix interaction domains on the collagen fibril, the predominant protein of vertebrates // J. Biol. Chem. 2008. Vol. 283. N 30. P. 21187–21197.
3. Schweizer J., Bowden P.E., Coulombe P.A., Langbein L., Lane E.B., Magin T.M., Maltais L., Omary M.B., Parry D.A.D., Rogers M.A., Wright M.W. New consensus nomenclature for mammalian keratins // J. Cell Biol. 2006. Vol. 174. N 2. P. 169–174.
4. Libby P., Bonow R.O., Mann D.L., Tomaselli G.F., Bhatt D., Solomon S.D., Braunwald E. Braunwald’s heart disease-e-book: A textbook of cardiovascular medicine. Elsevier, 2021. 2032 pp.
5. Chen N.C., Srinivasan R.C., Shauver M.J., Chung K.C. A systematic review of outcomes of fasciotomy, aponeurotomy, and collagenase treatments for Dupuytren’s contracture // Hand. 2011. Vol. 6. N 3. P. 250–255.
6. Erdeve O., Atasay B., Arsan S. Collagenase application for amputation in a preterm // Pediatr. Dermatol. 2007. Vol. 24. N 2. P. 195–196.
7. Hassan M.A., Abol-Fotouh D., Omer A.M., Tamer T.M., Abbas E. Comprehensive insights into microbial keratinases and their implication in various biotechnological and industrial sectors: A review // Int. J. Biol. Macromol. 2020. Vol. 154. P. 567–583.
8. Kumar J., Singh I., Kushwaha R.K.S. Keratinophilic fungi: Diversity, environmental and biotechnological implications // Progress in Mycology / Eds. T. Satyanarayana, S.K. Deshmukh, and M.V. Deshpande. Singapore: Springer, 2021. P. 419–436.
9. Popova E.A., Kreyer V.G., Komarevtsev S.K., Shabunin S.V., Osmolovskiy A.A. Properties of extracellular proteinase of the micromycete Aspergillus ustus 1 and its high activity during fibrillary-proteins hydrolysis // Appl. Biochem. Microbiol. 2021. Vol. 57. N 2. P. 200–205.
10. Kate S., Pethe A. Study of collagenase production by Penicillium sp. isolated from deteriorated leather sample // J. Adv. Sci. Res. 2022. Vol. 13. N 01. P. 227–234.
11. Cardoso K.B.B., Nascimento M.C., Batista A.C., de Melo Oliveira V., Nascimento T.P., da Silva Batista J.M., Costa R.M.P.B., Pastrana L., Porto A.L.F. Systematic analysis on the obtaining of fibrinolytic fungi enzymes // Res., Soc. Dev. 2022. Vol. 11. N 2: e13611225449.
12. Звонарева Е.С., Осмоловский А.А., Крейер В.Г., Баранова Н.А., Котова И.Б., Егоров Н.С. Продукция протеиназ с плазминоподобной и активирующей прекалликреин активностью микромицетом Aspergillus terreus // Прикл. биохим. микробиол. 2018. Т. 54. № 2. С. 195–200.
13. Попова Е.А., Осмоловский А.А., Крейер В.Г., Котова И.Б., Егоров Н.С. Продукция штаммом Аspergillus ustus протеиназ, высокоактивных в отношении фибриллярных белков // Микол. фитопатол. 2019. Т. 53. № 4. C. 229–235.
14. Osmolovskiy A.A., Schmidt L., Orekhova A.V., Komarevtsev S.K., Kreyer V.G., Shabunin S.V., Egorov N.S. Action of extracellular proteases of Aspergillus flavus and Aspergillus ochraceus micromycetes on plasma hemostasis proteins // Life. 2021. Vol. 11. N 8: 782.
15. Osmolovskiy A.A., Schmidt L., Orekhova A.V., Kreyer V.G., Baranova N.A., Egorov N.S. Effect of proteinase from Aspergillus fumigatus on blood plasma proteins // Moscow Univ. Biol. Sci. Bull. 2021. Vol. 76. N 2. P. 71–76.
16. Smith P.K., Krohn R.I., Hermanson G.T., Mallia A.K., Gartner F.H., Provenzano M.D., Fujimoto E.K., Goeke N.M., Olson B.J., Klenk D.C. Measurement of protein using bicinchoninic acid // Anal. Biochem. 1985. Vol. 150. N 1. P. 76–85.
17. Фокичев Н.С., Корниенко Е.И., Шаркова Т.С., Крейер В.Г., Осмоловский А.А. Тромболитическая активность и свойства препарата протеиназ, образуемых микромицетом Tolypocladium inflatum k1 // Микол. фитопатол. 2021. Т. 55. № 6. С. 449–456.
18. Kothary M.H., Chase T. Jr., Macmillan J.D. Correlation of elastase production by some strains of Aspergillus fumigatus with ability to cause pulmonary invasive aspergillosis in mice // Infect. Immun. 1984. Vol. 43. N 1. P. 320–325.
19. Tamura Y., Suzuki S., Sawada T. Role of elastase as a virulence factor in experimental Pseudomonas aeruginosa infection in mice // Microb. Pathog. 1992. Vol. 12. N 3. P. 237–244.
20. Khosravi A.R., Mahdavi Omran S., Shokri H., Lotfi A., Moosavi Z. Importance of elastase production in development of invasive aspergillosis // J. Mycol. Med. 2012. Vol. 22. N 2. P. 167–172.
Review
For citations:
Timorshina S.N., Popova E.A., Galiakberova A.A., Ochneva A.G., Osmolovskiy A.A. Aspergillus proteolytic enzymes hydrolyzing fibrillar proteins for biomedicine and biotechnological process. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2022;77(3):195-200. (In Russ.)
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