Effect of single-strand DNA breaks on transcription of nucleosomes
https://doi.org/10.55959/MSU0137-0952-16-2022-77-4-241-247
Abstract
Previous studies revealed the inhibitory effect of single-stranded breaks in a non-template DNA strand (NT-SSB) on RNA polymerase transcription through the nucleosome. The observed effect was explained within the model of chromatin transcription mechanism with the formation of intranucleosomal DNA loops (i-loops) – intermediates in which the enzyme is locked in the DNA loop on the histone octamer. According to the model, NT-SSBs reduce the tension in the DNA structure caused by transcription and hinders the opening of the i-loop with the enzyme and further elongation. In this work, the boundaries of such i-loops are determined. Nucleosomes with NT-SSBs were transcribed. It was determined that the formation of contacts between DNA and histones behind RNAP occurs at a distance of more than 17 base pairs from the active center of the enzyme.
About the Authors
N. S. Gerasimova
Lomonosov Moscow State University
Russian Federation
Russian Federation
Department of Bioengineering, School of Biology
1–12 Leninskie gory, Moscow, 119234
M. S. Akhtar
CSIR-Central Drug Research Institute
India
India
Molecular and Structural Biology Division
Lucknow 226031, Uttar Pradesh
V. M. Studitsky
Fox Chase Cancer Center
United States
United States
Cancer Epigenetics Team
Cottman Avenue 333, Philadelphia, PA 19111
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Review
For citations:
Gerasimova N.S., Akhtar M.S., Studitsky V.M. Effect of single-strand DNA breaks on transcription of nucleosomes. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2022;77(4):241-247. (In Russ.) https://doi.org/10.55959/MSU0137-0952-16-2022-77-4-241-247
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