Effect of graphene derivatives on biofilm formation by Candida maltose
https://doi.org/10.55959/MSU0137-0952-16-79-3-7
Abstract
Graphene derivatives (oxide and its reduced form) are promising carbon nanomaterials (CNMs) used in industry, electronics, medicine and biotechnology. The aim of the work was to study the effect of graphene oxide (GO) and its reduced form (rGO) on the formation and eradication of Candida maltosa VKPM Y-194 biofilms, metabolic activity, intracellular ATP content and the permeability of the cytoplasmic membrane of biofilm cells. It was found that GO and rGO slightly suppress yeast biofilm formation, and the decrease in biofilm biomass during growth in the presence of GO is significantly greater than during cell growth with rGO. The destruction of mature 7-day yeast biofilms is slightly greater in the presence of CNMs than in the control, and significantly greater than that of 3-day ones. At the same time, the metabolic activity of biofilm cells, assessed by the reduction of tetrazolium salt (methyl thiazolyl tetrazolium reagent), upon contact of biofilm cells with CNM for 4 hours, significantly increased in 3-day biofilms exposed to rGO. The content of intracellular ATP in biofilms grown in the presence of CNMs exceeded that in the control, but was lower after 4-hour effect on mature biofilms grown in a nutrient medium without CNM. The greatest negative effect on the cytoplasmic membrane of biofilm cells, which was expressed in an increase in its permeability, was exerted by GO upon 4-hour exposure to a 7-day biofilm. It was found that the negative effect of CNMs on biofilms of C. maltosa VKPM Y-194 is more pronounced when exposed to GO than to rGO, and higher when exposed to 7-day biofilms than to 3-day ones. Complete inhibition of biofilm formation and complete eradication of mature biofilms under the effect of CNMs have not been established.
Supporting agencies: The research was funded by Russian Science Foundation, project number № 24-24-20008, https://rscf.ru/en/project/24-24-20008/, Perm Krai.
About the Authors
E. V. Pyankova
Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Center, Ural Branch of the Russian Academy of Sciences
Russian Federation
Russian Federation
Goleva st. 13, Perm, 614081
Yu. G. Maksimova
Institute of Ecology and Genetics of Microorganisms, Perm Federal Research Center, Ural Branch of the Russian Academy of Sciences ; Perm State National Research University
Russian Federation
Russian Federation
Goleva st. 13, Perm, 614081
Bukireva st. 15, Perm, 614990
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Review
For citations:
Pyankova E.V., Maksimova Yu.G. Effect of graphene derivatives on biofilm formation by Candida maltose. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2024;79(3):227-234. (In Russ.) https://doi.org/10.55959/MSU0137-0952-16-79-3-7
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