RAPD AND ISSR MARKERS FOR STUDY OF GENETIC VARIATION AMONG PARSLEY (PETROSELINUM CRISPUM (MILL.) NYM.) ACCESSIONS
https://doi.org/10.1234/XXXX-XXXX-2010-4-25-28
Abstract
Five RAPD primers out of 20 and 6 SSR primers out of 13 were selected to discriminate 33 accessions of parsley (Petroselinum crispum [Mill] Nym. ex A.W. Hill). In the total, 51 RAPD and 66 ISSR bands were generated with these primers. The UPGMA dendrogram based on the Jaccard's coefficients was constructed. The two clusters of leaf and root plant forms were the most distant at similarity 0,292 from each other. Consequently, root form-specific markers were observed. Not all flat leaf and curly-leaf forms have been clearly separated, but some of curly-leaf accessions formed their own sub-cluster.
About the Authors
A. S. Domblides
Всероссийский научно-исследовательский институт селекции и семеноводства овощных культур
Russian Federation
Russian Federation
E. A. Domblides
Всероссийский научно-исследовательский институт селекции и семеноводства овощных культур
Russian Federation
Russian Federation
В. V.A. Kharchenko
Всероссийский научно-исследовательский институт селекции и семеноводства овощных культур
Russian Federation
Russian Federation
G. A. Potekhin
Всероссийский научно-исследовательский институт селекции и семеноводства овощных культур
Russian Federation
Russian Federation
References
1. Williams J.G.K., Kubelik A.R., Livak K.J, Rafals- ky J.A., Tingey S.V. DNA polymorphisms amplified by ar¬bitrary primers are useful as genetic markers // Nucleic Acids Res. 1990. N 18. P. 6531—6535.
2. Welsh J., McClelland M. Fingerprinting genomes using PCR with arbitrary primers // Nucleic Acids Res. 1990. N 18. P. 7213—7218.
3. Zietkiewicz E, Rafalski A., Labuda D. Genome fin¬gerprinting by simple sequence repeats (SSR)-anchored po- lymerase chain reaction amplification // Genomics. 1994. N 20. P. 176—183.
4. Dellaporta S.L., Wood J., Hicks J.B. A plant DNA minipreparation: Version II // Plant Mol. Biol. Rep. 1983. N 1(4). P. 19—21.
5. Waugh R. RAPD Analysis: use for genome characterization, tagging traits and mapping // Plant Molecular Biology — A Laboratory Manual / Ed. S. Melody. Clark. Berlin; Heidelberg: Springer-Verlag, 1997. P. 305—333.
6. GenStat 6 Committee. The guide to GenStat release 6.1. VSN. Oxford: International Ltd., 2002.
7. Van de Peer Y, De Wachter R. TREECON for Win¬dows: A software package for the construction and drawing of evolutionary trees for the Microsoft Windows environment // Comput. Appl. Biosci. 1994. Vol. 10. P. 569—570.
8. Jaccard P. Nouvelles researches sur la distribution flo- rale // Bull. Soc. Vaud. Sci. Nat. 1908. Vol. 44. P. 223—270.
9. Lohwasser U., Dittbrenner A., Marthe F., Borner A. Taxonomy of plant genetic resources — an interaction of mor¬phological, molecular and phytochemical data // Eucarpia 19 Genetic Recourses Section/Ljubljana: Kmetijski ins titut Slovenije, 2009. P. 36.
Review
For citations:
Domblides A.S., Domblides E.A., V.A. Kharchenko , Potekhin G.A. RAPD AND ISSR MARKERS FOR STUDY OF GENETIC VARIATION AMONG PARSLEY (PETROSELINUM CRISPUM (MILL.) NYM.) ACCESSIONS. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2010;(4):25-28. (In Russ.) https://doi.org/10.1234/XXXX-XXXX-2010-4-25-28
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