Preview

Vestnik Moskovskogo universiteta. Seriya 16. Biologiya

Advanced search

PHYLOGENETIC RELATIONSHIPS IN FAGOPYRUM SPECIES BASED ON NUCLEOTIDE POLYMORPHISM OF b/c INTRON OF NAD1 GENE

https://doi.org/10.1234/XXXX-XXXX-2010-4-36-38

Abstract

The sequence analysis of b/c intron of nadl mitochondrial gene in 13 Fagopyrum species was carried out and demonstrated ability of this intron to use in phylogenetic studies. The length of the mitochondrial nadl b/c intron varied from 1217 bp in F. tataricum, F. cymosum, F. giganteum to 1239 bp in F. capillatum. Species- and accession- specific SNPs and indels were detected. For obtained molecular phylogeny of Fagopyrum species a good agreement with the recognized taxo- nomic divisions within the genus was observed. NJ and MP trees showed a clear clustering into two major genetic groups: cymosum and urophyllum. The urophyllum group was characterized by the presence of several synapomorphic characters including 11 bp insert flanked by directrepeats.

About the Authors

G. D. Kadyrova
Центр "Биоинженерия" РАН, г.Москва; Татарский научно-исследовательский институт сельского хозяйства, г. Казань
Russian Federation


N. N. Ryzhova
Центр "Биоинженерия" РАН, г.Москва
Russian Federation


E. Z. Kochieva
Центр "Биоинженерия" РАН, г.Москва
Russian Federation


References

1. Yamane K., Yasui Y, Ohnishi O. Intraspecific cpDNA gopyrum cymosum (Polygonaceae) // Am. J. Bot. 2003. Vol. 90. variation of diploid and tetraploid perennial buckwheat, Fa- P. 339—346.

2. Campbell C.G. Buckwheat. Fagopyrum esculentum Moench // International Plant Genetic Resources Institute. Rome, Italy, 1997. 95 p.

3. Ohsako T., Yamane K., Ohnishi O. Two new Fagopyrum (Polygonaceae) species F.gracilipedoides and F.jinshaense from Yunnan, China // Genes and Genet. Syst. 2002. N 77. P. 399—408.

4. Yasui Y., Ohnishi O. Interspecific relationships in Fagopyrum (Polygonaceae) revealed by the nucleotide sequen¬ces of the rbcL and accD genes and their intergenic region // Am. J. Bot. 1998a. Vol. 85. P. 1134—1142.

5. Cameron K.M. On the value of nuclear and mitochon- drial gene sequences for reconstructing the phylogeny of vanilloid orchids (Vanilloideae, Orchidaceae) // Ann. Bot. 2009. Vol. 104(3). P. 377—385.

6. Edwards K., Johnstone C., Thompson C. A simple and rapid method for the preparation of plant genomic DNA for PCR analysis // Nucl. Acids Res. 1991. Vol. 19(6). Ð. 1349.

7. Demesure B., Sodzi N., Petit R.J. A set of universal primers for amplication of polymorphic non-coding regions of mitochondrial and chloroplast DNA in plants // Molec. Ecol. 1995. Vol.4. P. 129—131.

8. Sharma T.R., Jana S. Species relationships in Fago- pyrum revealed by PCR-based DNA fingerprinting // Theor. Appl. Genet. 2002. Vol. 105. P. 306—312.


Review

For citations:


Kadyrova G.D., Ryzhova N.N., Kochieva E.Z. PHYLOGENETIC RELATIONSHIPS IN FAGOPYRUM SPECIES BASED ON NUCLEOTIDE POLYMORPHISM OF b/c INTRON OF NAD1 GENE. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2010;(4):36-38. (In Russ.) https://doi.org/10.1234/XXXX-XXXX-2010-4-36-38

Views: 281


ISSN 0137-0952 (Print)