COMPARATIVE ANALYSIS OF SERPINA1 GENE EXPRESSION IN TUMOR CELL LINES
Abstract
The expression of SERPINA1 gene in prostate prostate (DU145, PC-3 and LNCaP) and human liver (HepG2) tumor cell lines was studied. Alpha1-antitrypsin (AAT) level in the whole cell extracts, secretomes, subcellular fractions and SERPINA1 mRNA level in the corresponding cells were detected. Discordance between expression at these two levels in PC-3 and LNCaP lines was revealed. A new 37 KDa AAT N-terminus truncated isoform was detected in the nuclear extracts of some prostate tumor cell lines. The mechanism of 37 KDa AAT isoform intracellular retention was proposed. Two polyadenylation sites in the 3-untranslated region of SERPINA1 transcripts were identified. A SERPINA1 gene 3-untranslated region influence on AAT translation has been discussed.
About the Authors
A. A. Maslakova
биологический факультет МГУ
Russian Federation
Russian Federation
M. V. Telkov
ФНИЦ эпидемиологии и микробиологии им. Н.Ф. Гамалеи
Russian Federation
Russian Federation
I. V. Orlovsky
НИИ Физико-химической биологии имени А.Н. Белозерского МГУ
Russian Federation
Russian Federation
O. S. Sokolova
биологический факультет МГУ
Russian Federation
Russian Federation
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Review
For citations:
Maslakova A.A., Telkov M.V., Orlovsky I.V., Sokolova O.S. COMPARATIVE ANALYSIS OF SERPINA1 GENE EXPRESSION IN TUMOR CELL LINES. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2015;(3):26-31. (In Russ.)
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