DETECTION OF ANAEROBIC SULFATE-REDUCING BACTERIA IN OXYGEN-CONTAINING UPPER WATER LAYERS OF THE BLACK AND BALTIC SEAS
Abstract
Fluorescent in situ hybridization (FISH) and PCR were used for analysis of the phylogenetic structure of anaerobic sulfate-reducing bacterial communities in oxygen-containing upper water layers of meromictic basins — the Black Sea and the Gdańsk Deep of the Baltic Sea. In the Black Sea (continental slope at depths 30–70 m), cells of sulfate-reducing bacteria (SRB) hybridizing with 16S rRNAspecific FISH-probes for Desulfotomaculum, Desulfobacter and Desulfovibrio genera were revealed, whereas Desulfomicrobium-related bacteria were prevalent in the chemocline zone at 150-m depth. Besides of Desulfotomaculum (SRB subgroup 1), Desulfobacter (SRB subgroup 4) and Desulfovibrio-Desulfomicrobium (SRB subgroup 6), nested PCR with the use of 16S rRNA gene-specific primers detected the presence of Desulfococcus-Desulfonema-Desulfosarcina (SRB subgroup 5) in oxygen-containing water column of the Black and Baltic seas. Active enrichment SRB culture which contained bacterium Desulfosporosinus sp. as a major component was obtained from the Black Sea water sample collected at 70-m depth.
About the Authors
A. L. Bryukhanov
кафедра микробиологии биологического факультета МГУ
Russian Federation
Russian Federation
V. A. Korneeva
кафедра микробиологии биологического факультета МГУ
Russian Federation
Russian Federation
N. V. Pimenov
Институт микробиологии им. С.Н. Виноградского РАН, г. Москва
кафедра микробиологии биологического факультета МГУ
Russian Federation
Russian Federation
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Review
For citations:
Bryukhanov A.L., Korneeva V.A., Pimenov N.V. DETECTION OF ANAEROBIC SULFATE-REDUCING BACTERIA IN OXYGEN-CONTAINING UPPER WATER LAYERS OF THE BLACK AND BALTIC SEAS. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2015;(4):36-40. (In Russ.)
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