STUDY OF RNA POLYMERASE TRANSCRIPTION THROUGH NUCLEOSOME BY CRYO-ELECTRON MICROSCOPY APPROACH
Abstract
One of the most promising and reliable methods for analysis of the structure of macromolecules is cryo-electron transmission microscopy with subsequent three-dimensional reconstruction. In this study, this method was used to study transcription by RNA polymerase, stalled at the position 42 bp into a nucleosome. We obtained the projection images and solved the three-dimensional structure of the complex EC-42 with the 2.5 nm resolution. It allowed us to confirm the conformational integrity of the nucleosome, during the passage of RNA polymerase.
About the Authors
O. I. Volokh
Department of Bioengineering, School of Biology, M.V. Lomonosov Moscow State University, Leninskiye gory 1–12, Moscow, 119234, Russia
Russian Federation
Russian Federation
K.-F. Hsieh
Robert Wood Johnson Medical School, Rutgers University, Paterson Str. 125, New Brunswick, NJ 08901, USA
Russian Federation
Russian Federation
M. G. Karlova
Department of Bioengineering, School of Biology, M.V. Lomonosov Moscow State University, Leninskiye gory 1–12, Moscow, 119234, Russia
Russian Federation
Russian Federation
E. S. Trifonova
Department of Bioengineering, School of Biology, M.V. Lomonosov Moscow State University, Leninskiye gory 1–12, Moscow, 119234, Russia
Russian Federation
Russian Federation
V. M. Studitsky
Cancer Epigenetics Team, Fox Chase Cancer Center, Cottman Avenue 333, Philadelphia, PA 19111, USA
Russian Federation
Russian Federation
O. S. Sokolova
Department of Bioengineering, School of Biology, M.V. Lomonosov Moscow State University, Leninskiye gory 1–12, Moscow, 119234, Russia
Russian Federation
Russian Federation
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Review
For citations:
Volokh O.I., Hsieh K., Karlova M.G., Trifonova E.S., Studitsky V.M., Sokolova O.S. STUDY OF RNA POLYMERASE TRANSCRIPTION THROUGH NUCLEOSOME BY CRYO-ELECTRON MICROSCOPY APPROACH. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2016;(1):41-45. (In Russ.)
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