INFLUENCE OF TETRAPEPTIDE TUFTSIN ON INTRACELLULAR PH OF MOUSE PERITONEAL MACROPHAGES
Abstract
It was demonstrated by means of probe microfluorimetry of single cells that tuftsin in concentrations 0.1 and 1.0 ug/ml (that increase murine peritoneal macrophages phagocytic activity) changed intracellular pH (pHi) biphasicaly over time. At the beginning there was a decrease of pHi reaching the ultimate expression after 5 min of incubation. After that pHi value increased, reaching maximum 30 min later. The observed parameter (pHi) did not change up to 55 min. It was found that after administration of the Na+/H+ exchange blocker ethylisopropylamiloride into the cells incubation medium during tuftsin treatment there was no increase of intracellular pH. This finding can be the evidence that the observed increase of pHi during tuftsin treatment through the second phase of cellular response connected with the system of Na+/H+ exchange.
About the Authors
S. K. Pirutin
Department of Biophysics and School of Biology, M.V. Lomonosov Moscow State University, Leninskiye gory 1–12, Moscow, 119234, Russia
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Institutskaya ul. 3, Pushchino, Moscow region, 142290, Russia
Russian Federation
Russian Federation
V. B. Turovetsky
Department of Biophysics and School of Biology, M.V. Lomonosov Moscow State University, Leninskiye gory 1–12, Moscow, 119234, Russia
Russian Federation
Russian Federation
N. Y. Sarycheva
Department of Human and Animal Physiology, School of Biology, M.V. Lomonosov Moscow State University, Leninskiye gory 1–12, Moscow, 119234, Russia
Russian Federation
Russian Federation
A. B. Druzhko
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, Institutskaya ul. 3, Pushchino, Moscow region, 142290, Russia
Russian Federation
Russian Federation
V. N. Kalihevich
Department of Natural Compounds Chemistry, Institute of Chemistry, Saint-Petersburg State
University, Universitetsky pr. 26, Peterhof, St. Petersburg, 198504, Russia
Russian Federation
Russian Federation
A. A. Kamensky
Department of Human and Animal Physiology, School of Biology, M.V. Lomonosov Moscow State University, Leninskiye gory 1–12, Moscow, 119234, Russia
Russian Federation
Russian Federation
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Review
For citations:
Pirutin S.K., Turovetsky V.B., Sarycheva N.Y., Druzhko A.B., Kalihevich V.N., Kamensky A.A. INFLUENCE OF TETRAPEPTIDE TUFTSIN ON INTRACELLULAR PH OF MOUSE PERITONEAL MACROPHAGES. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2016;(1):66-70. (In Russ.)
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