FIBRINOLYTIC AND COLLAGENOLYTIC ACTIVITY OF EXTRACELLULAR PROTEINASES OF MICROMYCETES ASPERGILLUS OCHRACEUS L-1 AND ASPERGILLUS USTUS 1
Abstract
It was shown that extracellular proteinases produced by micromycetes A. ochraceus L-1 and A. ustus 1 differ by the values of activity at various pH values and the intensity of its effect on fibrillar proteins. It was revealed that the proteinase of A. ochraceus L-1 demonstrated maximum activity during the growth of the producer on nitrate-free growth medium, at pH 8.0, and proteinase of A. ustus 1 showed maximum activity during the growth of the micromycete on a medium containing sodium nitrate, at pH of 6.0. Values of specific fibrinolytic and collagenolytic activities of A. ochraceus L-1 were in 2.2 and 1.6 times greater than those activities of A. ustus 1, but A. ustus 1 showed low values of total proteolytic (caseinolytic) activity and had a high ratio of fibrinolytic activity to total proteolytic (caseinolytic) activity (6.92), making it a promising producer of proteinases which hydrolyze fibrin and collagen.
About the Authors
A. A. Osmolovskiy
Department of Microbiology, School of Biology, M.V. Lomonosov Moscow State University, Leninskiye gory 1–12, Moscow, 119234, Russia
Russian Federation
Russian Federation
E. A. Popova
Department of Microbiology, School of Biology, M.V. Lomonosov Moscow State University, Leninskiye gory 1–12, Moscow, 119234, Russia
Russian Federation
Russian Federation
V. G. Kreyer
Department of Microbiology, School of Biology, M.V. Lomonosov Moscow State University, Leninskiye gory 1–12, Moscow, 119234, Russia
Russian Federation
Russian Federation
N. A. Baranova
Department of Microbiology, School of Biology, M.V. Lomonosov Moscow State University, Leninskiye gory 1–12, Moscow, 119234, Russia
Russian Federation
Russian Federation
N. S. Egorov
International Biotechnological Center, M.V. Lomonosov Moscow State University, Leninskiye gory 1–12, Moscow, 119234, Russia
Russian Federation
Russian Federation
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Review
For citations:
Osmolovskiy A.A., Popova E.A., Kreyer V.G., Baranova N.A., Egorov N.S. FIBRINOLYTIC AND COLLAGENOLYTIC ACTIVITY OF EXTRACELLULAR PROTEINASES OF MICROMYCETES ASPERGILLUS OCHRACEUS L-1 AND ASPERGILLUS USTUS 1. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2016;(1):71-75. (In Russ.)
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