MOLECULAR AND BIOLOGICAL CHARACTERIZATION OF NOVEL ISOLATES OF PLUM POX VIRUS STRAIN WINONA
Abstract
Plum pox virus (PPV, genus Potyvirus, family Potyviridae) is considered to be the most detrimental viral pathogen of stone fruit crops (Prunus spp.). The strain Winona (PPV-W) is the most variable strain of the nine recognized strains of the virus and is one of the most common in European Russia. Six new PPV-W isolates were first found in decorative green plantings of the city of Moscow (Kp2U, Avang, Pulk, Pulk-1), also in the Taldom district of the Moscow region (Karm) and in the Kovrov district of the Vladimir region (Vlad-4) on wild plums Prunus domestica. Analysis of the 3’-terminal genome segment of the novel isolates confirmed the high level of the PPV-W intra-strain diversity. Study of their relationship with other PPV-W isolates using the phylogenetic analysis of the coat protein gene’s sequences revealed no geographical clustering of the Russian PPV-W isolates. The aphid and seed transmission of the PPV-W was also investigated. Inoculation of Nicotiana benthamiana plants by the hop aphid Phorodon humuli from the plums, infected with the isolates Avang and Pulk, and by the thistle aphid Brachycaudus cardui from the plum, infected with the isolate Kp2U, was shown to result in the systemic infection of tobacco plants thus indicating the possibility of the natural PPV-W transmission by both aphid species. No evidence of the PPV-W seed transmission has been obtained.
About the Authors
A. V. Zakubanskiy
Department of Virology, School of Biology, Lomonosov Moscow State University,
Leninskiye gory 1-12, Moscow, 119234, Russia
Russian Federation
Russian Federation
A. A. Sheveleva
Department of Virology, School of Biology, Lomonosov Moscow State University,
Leninskiye gory 1-12, Moscow, 119234, Russia
Russian Federation
Russian Federation
S. N. Chirkov
Department of Virology, School of Biology, Lomonosov Moscow State University, Leninskiye gory 1-12, Moscow, 119234, Russia
Russian Federation
Russian Federation
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Review
For citations:
Zakubanskiy A.V., Sheveleva A.A., Chirkov S.N. MOLECULAR AND BIOLOGICAL CHARACTERIZATION OF NOVEL ISOLATES OF PLUM POX VIRUS STRAIN WINONA. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2016;(2):8-12. (In Russ.)
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