OCCURRENCE OF GRAPEVINE LEAFROLL-ASSOCIATED VIRUSES-1 AND -3 IN CRIMEA
Abstract
Grapevine leafroll is one of the most widespread and harmful diseases of grapevine. To investigate the occurrence of grapevine leafroll-associated viruses-1 и 3, the survey of vineyards has been conducted in 2015 in six regions of Crimea. Total of 689 leaf samples with virus symptoms were collected. GLRaV-1 and GLRaV-3 were analysed by RT-PCR with the specific primers, followed by sequencing of the PCR products. GLRaV-1 and GLRaV-3 were detected in 34 (4.9%) and 37 (5.4%) of the samples, respectively. Vineyards in Simferopol, Bahchisaray and Sevastopol regions were found to be free of GLRaV-1 and GLRaV-3.
About the Authors
E. V. Porotikova
Research Center of Biotechnology, RAS, 33-2 Leninsky Prospect, Moscow, 119071, Russia
Russian Federation
Russian Federation
V. I. Risovannaya
All-Russian National Research Institute for Viticulture and Wine-making “Magarach”, 31 Kirov Ul., Yalta, Republic of the Crimea, 298600, Russia
Russian Federation
Russian Federation
Y. A. Volkov
All-Russian National Research Institute for Viticulture and Wine-making “Magarach”, 31 Kirov Ul., Yalta, Republic of the Crimea, 298600, Russia
Russian Federation
Russian Federation
U. D. Dmitrenko
Research Center of Biotechnology, RAS, 33-2 Leninsky Prospect, Moscow, 119071, Russia
Russian Federation
Russian Federation
V. A. Volodin
All-Russian National Research Institute for Viticulture and Wine-making “Magarach”, 31 Kirov Ul., Yalta, Republic of the Crimea, 298600, Russia
Russian Federation
Russian Federation
S. M. Gorislavets
All-Russian National Research Institute for Viticulture and Wine-making “Magarach”, 31 Kirov Ul., Yalta, Republic of the Crimea, 298600, Russia
Russian Federation
Russian Federation
E. P. Stranishevskaya
All-Russian National Research Institute for Viticulture and Wine-making “Magarach”, 31 Kirov Ul., Yalta, Republic of the Crimea, 298600, Russia
Russian Federation
Russian Federation
A. A. Agranovsky
Department of Virology, School of Biology, Lomonosov Moscow State University, 1-12 Leninskiye Gory, Moscow, 119991, Russia
Russian Federation
Russian Federation
A. M. Kamionskaya
Research Center of Biotechnology, RAS, 33-2 Leninsky Prospect, Moscow, 119071, Russia
Russian Federation
Russian Federation
S. M. Vinogradova
Research Center of Biotechnology, RAS, 33-2 Leninsky Prospect, Moscow, 119071, Russia
Russian Federation
Russian Federation
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For citations:
Porotikova E.V., Risovannaya V.I., Volkov Y.A., Dmitrenko U.D., Volodin V.A., Gorislavets S.M., Stranishevskaya E.P., Agranovsky A.A., Kamionskaya A.M., Vinogradova S.M. OCCURRENCE OF GRAPEVINE LEAFROLL-ASSOCIATED VIRUSES-1 AND -3 IN CRIMEA. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2016;(2):13-16. (In Russ.)
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