HYDROXYL RADICAL FOOTPRINTING OF FLUORESCENT-LABELED DNA
Abstract
Footprinting is one of the simplest and most accurate approaches to investigate structure and interaction of biopolymers. It is based on the accessibility of intra- and intermolecular contacts for external damaging agents. In the method, one end of the polymer is labeled, and then the sample is incubated in cutting medium. Length distribution of the products allows to reveal the accessibility of different regions of polymer in the corresponding conditions. In DNA footprinting various enzymes and chemical reagents can be used. The highest temporal and spatial resolution without sequence specificity can be obtained with hydroxyl radicals. In this paper we present a new modification of the experimental approach using fluorescent-labeled DNA fragments and up-to-date methods of quantitative analysis, which can considerably increase its applicability.
About the Authors
N. S. Gerasimova
Department of Bioengineering, School of Biology, Lomonosov Moscow State University, Leninskiye gory 1–12, Moscow, 119234, Russia
Russian Federation
Russian Federation
V. M. Studitsky
Department of Bioengineering, School of Biology, Lomonosov Moscow State University, Leninskiye gory 1–12, Moscow, 119234, Russia
Cancer Epigenetics Program Team, Fox Chase Cancer Center, Cottman Avenue 333, Philadelphia, PA 19111, USA
Russian Federation
Russian Federation
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Review
For citations:
Gerasimova N.S., Studitsky V.M. HYDROXYL RADICAL FOOTPRINTING OF FLUORESCENT-LABELED DNA. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2016;(2):32-36. (In Russ.)
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