SCREENING OF CULTURE MEDIA WITH DIFFERENT NUTRIENT CONTENT FOR CULTIVATION OF MICROALGAE ASSOCIATED WITH WHITE SEA BENTHIC INVERTEBRATES
Abstract
The growth and biomass accumulation of three microalgal strains of Desmodesmus (Scenedesmaceae, Chlorophyceae), 1Рm66В, 2Cl66E, 3Dp86Е-1, isolated from White Sea benthic invertebrates were studied under conditions of batch culture in different standard (BG-11, Prat, Goldberg, Gromov, Tamiya, artificial sea water) and modified media. Culture condition and biomass accumulation were recorded as well as the uptake of nitrate and phosphate. Vigorous growth of the microalgae brought about a significant alkalization of the culture medium to pH 10. The most significant biomass accumulation was recorded in BG-11 (the complete medium and one with addition of artificial sea water), Tamiya and Prat media. Addition of the sea water did not affect the growth of Desmodesmus sp. in the nitrate-containing media although that maintained the growth of the microalgae in the nitrogen-lacking media without cell aggregation. The obtained results suggest the suitability of BG-11 medium for isolation and cultivation of both symbiotic and free-living microalgae. The Prat medium is more suitable for maintaining the microalgal strains in collection.
About the Authors
E. S. Lobakova
Department of Bioengineering, School of Biology, Lomonosov Moscow State University, Leninskiye gory 1–12, Moscow, 119234, Russia
Russian Federation
Russian Federation
I. O. Selyakh
Department of Bioengineering, School of Biology, Lomonosov Moscow State University, Leninskiye gory 1–12, Moscow, 119234, Russia
Russian Federation
Russian Federation
L. R. Semenova
Department of Bioengineering, School of Biology, Lomonosov Moscow State University, Leninskiye gory 1–12, Moscow, 119234, Russia
Russian Federation
Russian Federation
O. B. Chivkunova
Department of Bioengineering, School of Biology, Lomonosov Moscow State University, Leninskiye gory 1–12, Moscow, 119234, Russia
Russian Federation
Russian Federation
P. N. Scherbakov
Department of Bioengineering, School of Biology, Lomonosov Moscow State University, Leninskiye gory 1–12, Moscow, 119234, Russia
Russian Federation
Russian Federation
A. E. Solovchenko
Department of Bioengineering, School of Biology, Lomonosov Moscow State University, Leninskiye gory 1–12, Moscow, 119234, Russia
Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya ul. 35, Moscow, 127276, Russia
Russian Federation
Russian Federation
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Review
For citations:
Lobakova E.S., Selyakh I.O., Semenova L.R., Chivkunova O.B., Scherbakov P.N., Solovchenko A.E. SCREENING OF CULTURE MEDIA WITH DIFFERENT NUTRIENT CONTENT FOR CULTIVATION OF MICROALGAE ASSOCIATED WITH WHITE SEA BENTHIC INVERTEBRATES. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2016;(2):43-48. (In Russ.)
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