INDUCTION OF TELOMERASE ACTIVITY INCREASE REPROGRAMMING EFFICIENCY OF HUMAN DERMAL FIBROBLASTS
https://doi.org/10.1234/XXXX-XXXX-2012-1-8-14
Abstract
Reprogramming of human cells are a perspective direction of development of cellular biology with distant prospect of use of the given methods for cellular-replacing therapy in clinical practice. One of problems rising on this way is low reprogramming efficiency and application of undesirable oncogenes in initial techniques. In this research we had been offered the alternative modified method of reprogramming human skin fibroblasts. It has been shown that the telomerase induction increases an exit of reprogrammed cells and allows to exclude oncogen c-Myc from a used set of genes.
About the Authors
E. B. DashinimaevRussian Federation
I. A. Muchkaeva
Russian Federation
R. R. Faizullin
Russian Federation
Y. Y. Yegorov
Russian Federation
S. S. Akimov
Russian Federation
V. V. Terskikh
Russian Federation
A. V. Vasiliev
Russian Federation
M. P. Kirpichnikov
Russian Federation
References
1. Campbell K.H., McWhir J., Ritchie W.A., Wilmut I. Sheep cloned by nuclear transfer from a cultured cell line // Nature. 1996. Vol. 380. P. 64—66.
2. Byrne J.A., Pedersen D.A., Clepper L.L., Nelson M., Sanger W.G., Gokhale S., Wolf D.P., Mitalipov S.M. Producing primate embryonic stemm cells by somatic cell nuclear transfer // Nature. 2007. Vol. 450(7169). P. 497—502.
3. Tada M., Takahama Y., Abe K., Nakatsuji N., Tada T. Nuclear reprogramming of somatic cells by in vitro hybridization with ES cells // Curr. Biol. 2001. Vol. 11. P. 1553—1558.
4. Takahashi K., Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors // Cell. 2006. Vol. 126(4). P. 663—676.
5. Takahashi K., Yamanaka S. Induction of pluripotent stem cells from adult human fibroblasts by defined factors // Cell. 2007. Vol. 131(5). P. 861—872.
6. Okita K., Nakagawa M., Hyenjong H., Ichisaka T., Yamanaka S. Generation of mouse induced pluripotent stem cells without viral vectors // Science. 2008. Vol. 322(5903). P. 949—953.
7. Nakagawa M., Koyanagi M., Tanabe K., Takahashi K., Ichisaka T., Aoi T., Okita K., Mochiduki Y. Takizawa N., Yamanaka S. Generation of induced pluripotent stem cells without Myc from mouse and human fibroblasts // Nature Biotechnology. 2008.Vol. 26. P. 101—106.
8. Mathew R., Jia W., Sharma A., Zhao Y., Clarke L.E., Cheng X., Wang H., Salli U., Vrana K.E., Robertson G.P., Zhu J., Wang S. Robust activation of the human but not mouse telomerase gene during the induction of pluripotency // FASEB J. 2010. Vol. 24. P. 2702—2715.
9. Agarwal S., Loh Y.H., McLoughlin E.M., Huang J., Park I.H., Miller J.D., Huo H., Okuka M., Dos Reis R.M., Loewer S., Ng H.H., Keefe D.L., Goldman F.D., Klingelhutz A.J., Liu L., Daley G.Q. Telomere elongation in induced pluripotent stem cells from dyskeratosis congenita patients // Nature. 2010. Vol. 464 P. 292—296.
10. Utikal J., Polo J.M., Stadtfeld M., Maherali N., Kulalert W., Walsh R.M., Khalil A., Rheinwald J.G., Hochedlinger K. Immortalization eliminates a roadblock during cellular reprogramming into iPS cells // Nature. 2009. Vol. 460. P. 1145—1148.
11. Yehezkel S., Rebibo-Sabbah A., Segev Y., Tzukerman M., Shaked R., Huber I., Gepstein L., Skorecki K., Selig S. Reprogramming of telomeric regions during the generation of human induced pluripotent stem cells and subsequent differentiation into fibroblast-like derivatives // Epigenetics. 2011. Vol. 6. P. 63—75.
12. Scheper W., Copray S. The molecular mechanism of indused pluripotency: a two-stage switch // Stem Cell Rev. 2009. Vol. 5(3). P. 204—223.
13. Patel M., Yang S. Advances in reprogramming somatic cells to induced pluripotent stem cells // Stem Cell Rev. 2010. Vol. 6(3). P. 367—380.
14. Егоров Е.Е. Теломераза, старение, рак // Молекулярная биология. 1997. Т. 31. С. 16—25.
15. Егоров Е.Е., Молдавер М.В., Вишнякова Х.С., Терехов С.М., Дашинимаев Э.Б., Чеглаков И.Б., Торопыгин И.Ю., Ярыгин К.Н., Чумаков П.М., Корочкин Л.И., Антонова Г.А., Рыбалкина Е.Ю., Сабурина И.Н., Бурнаевский Н.С., Зеленин А.В. Усиление контроля пролиферации в теломеризованых клетках // Онтогенез. 2007. Т. 38, № 2. С. 105—19.
16. Armstrong L., Saretzki G., Peters H., Wappler I., Evans J., Hole N., von Zglinicki T., Lako M. Overexpression of telomerase confers growth advantage, stress resistance, andenhanced differentiation of ESCs toward the hematopoietic lineage// Stem Cells. 2005. Vol. 23(4). P. 516—529.
17. Zvereva M.I., Shcherbakova D.M., Dontsova O.A. Telomerase: structure, functions, and activity regulation // Biochemistry. 2010. Vol. 75(13). P. 1563—83.
18. Bryan T.M., Englezou A., Gupta J., Bacchetti S., Reddel R.R. Telomere elongation in immortal human cells without detectable telomerase activity // EMBO J. 1995. Vol. 14(17). P. 4240—4248.
19. Wright W.E., Shay J.W. Telomere positional effects and the regulation of cellular senescence // Trends Genet. 1992. Vol. 8(6). P. 193—197.
Review
For citations:
Dashinimaev E.B., Muchkaeva I.A., Faizullin R.R., Yegorov Y.Y., Akimov S.S., Terskikh V.V., Vasiliev A.V., Kirpichnikov M.P. INDUCTION OF TELOMERASE ACTIVITY INCREASE REPROGRAMMING EFFICIENCY OF HUMAN DERMAL FIBROBLASTS. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2012;(1):8-14. (In Russ.) https://doi.org/10.1234/XXXX-XXXX-2012-1-8-14
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