MOLECULAR GENETIC FEATURES OF 5S rDNA NON-TRANSCRIBED SPACER IN HIPPOPHAE RHAMNOIDES L.
Abstract
Amplification of sea buckthorn Hippophae rhamnoides L. 5S rDNA non-transcribed spacer with coding border anneal primers showed existence of single fragment. The fragment was cloned and sequenced. It was shown that length of the Hippophae rhamnoides L. 5S rDNA nontranscribed spacer is 807 bp. Analysis of the sequence allowed to detect a high homology with early described microsatellite locuses of Hippophae rhamnoides L., russian olive Elaeagnus angustifolia L. and Calligonum mongolicum Turcz., that include a (GA)9 motif. These results may be useful to study a ribosomal RNA gene organization.
About the Authors
O. S. Alexandrov
Russian State Agrarian University — Moscow Timiryazev Agricultural Academy, Moscow
Russian Federation
Centre for Molecular Biotechnology
Russian Federation
Centre for Molecular Biotechnology
A. V. Evtukhov
Russian State Agrarian University — Moscow Timiryazev Agricultural Academy, Moscow
Russian Federation
Centre for Molecular Biotechnology
Russian Federation
Centre for Molecular Biotechnology
I. I. Kiselev
Russian State Agrarian University — Moscow Timiryazev Agricultural Academy, Moscow
Russian Federation
Centre for Molecular Biotechnology
Russian Federation
Centre for Molecular Biotechnology
G. I. Karlov
Russian State Agrarian University — Moscow Timiryazev Agricultural Academy, Moscow
Russian Federation
Centre for Molecular Biotechnology
Russian Federation
Centre for Molecular Biotechnology
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Review
For citations:
Alexandrov O.S., Evtukhov A.V., Kiselev I.I., Karlov G.I. MOLECULAR GENETIC FEATURES OF 5S rDNA NON-TRANSCRIBED SPACER IN HIPPOPHAE RHAMNOIDES L. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2016;(4):57-60. (In Russ.)
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