ANOMALIES OF THE EARLY STAGES OF DEVELOPMENT OF ERYSIPHE GRAMINIS TRITICI UNDER OXIDATIVE STRESS
Abstract
We report on investigation of early stage interactions between powdery mildew pathogen and host plant. We demonstrated that treatment of wheat leaves with various concentrations of hydrogen peroxide and 3-amino-1,2,4-triazole resulted in formation of morphological anomalies of germ tubes and non-viable colonies on host plant leaves. The observed effect of oxidative stress on germination anomalies of powdery mildew is similar to previously reported interactions between the pathogen and mildew resistant plants. Based on this work we conclude that abnormal infectious structure formation of wheat powdery mildew may be associated with increased presence of reactive oxygen species during plant defense responses.
About the Authors
G. A. Avetisyan
N.V. Tsitsin Main Botanical Garden of Russian Academy of Sciences
Russian Federation
Russian, 127276, Moscow, Botanicheskaya ul., 4
Russian Federation
Russian, 127276, Moscow, Botanicheskaya ul., 4
T. V. Avetisyan
N.V. Tsitsin Main Botanical Garden of Russian Academy of Sciences
Russian Federation
Russian, 127276, Moscow, Botanicheskaya ul., 4
Russian Federation
Russian, 127276, Moscow, Botanicheskaya ul., 4
References
1. Huckelhoven R., Fodor J., Preis C., Kogel K.H. Hypersensitive cell death and papilla formation in barley attacked by the powdery mildew fungus are associated with hydrogen peroxide but not with salicylic acid accumulation // Plant Physiol. 1999. Vol. 119. N 4. P. 1251–1260.
2. Trujillo M., Altschmied L., Schweizer P., Kogel K.H., H ckelhoven R. Respiratory burst oxidase homologue A of barley contributes to penetration by the powdery mildew fungus Blumeria graminis f. sp. hordei // J. Exp. Bot. 2006. Vol. 57. N 14. P. 3781–3791.
3. Huckelhoven R. Powdery mildew susceptibility and biotrophic infection strategies // FEMS Microbiol. Lett. 2005. Vol. 245. N 1. P. 9–17.
4. Шкаликов В.А., Дьяков Ю.Т., Смирнов А.Н., Джалилов Ф.С.-У., Стройков Ю.М., Коновалов Ю.Б., Гриценко В.В. Иммунитет растений. Москва: КолосС, 2005. 190 с.
5. Kunoh H., Kunoh K., Ishizaki H. Cytological studies of the early stages of powdery mildew in barley and wheat. XI. Autoflyorescence and galos at penetration sites of appressoria of Erysiphe graminis hordei and Erysiphe pisi on barley coleoptiles // Can. J. Bot. 1985. Vol. 63. N 9. P. 1535–1539.
6. Шервуд Р.Т., Вэнс К.П. Первичные изменения в клетках эпидермиса при проникании паразита // Инфекционные болезни растений: физиологические и биохимические основы / Под ред. Ю.Т. Дьякова. М.: Агропромиздат, 1985. С. 34–53.
7. Vanacker H., Carver T.L.W., Foyer C.H. Early H2O2 accumulation in mesophyll cells leads to induction of glutathione during the hyper-sensitive response in the barleypowdery mildew interaction // Plant Physiology. 2000. Vol. 123. N 4. P. 1289–1300.
8. Walters D., Cowley T., Mitchell A. Methyl jasmonate alters polyamine metabolism and induces systemic protection against powdery mildew infection in barley seedlings // J. Exp. Bot. 2002. Vol. 53. N 369. P. 747–756.
9. Feng H., Li X., Duan J., Li H., Liang H. Chilling tolerance of wheat seedlings is related to an enhanced alternative respiratory pathway // Crop Sci. 2008. Vol. 48. N 6. P. 2381–2388.
10. Shetty N.P., Lyngs J rgensen H.J., Jensen J.D., Collinge D.B., Shetty H.S. Roles of reactive oxygen species in interactions between plants and pathogens // Eur. J. Plant Pathol. 2008. Vol. 121. N 3. P. 267–280.
11. Perez F.J., Rubio S.N. An improved chemiluminescence method for hydrogen peroxide determination in plant tissues // Plant Growth Regul. 2006. Vol. 48. N 1. P. 89–95.
12. Аветисян Г.А. Цитофизиологические особенности ранних стадий развития возбудителя мучнистой росы пшеницы при моделировании окислительного стресса: дисс. канд. биол. наук. Москва, 2011. 130 с.
13. Hückelhoven R., Dechert C., Kogel K-H. Overexpression of barley BAX inhibitor 1 induces breakdown of mlo-mediated penetration resistance to Blumeria graminis // Proc. Natl. Acad. Sci. U.S.A. 2003. Vol. 100. N 9. P. 5555– 5560.
14. Дьяков Ю.Т., Озерецковская О.Л., Джавахия В.Г., Багирова С.Ф. Общая и молекулярная фитопатология. М.: Общество фитопатологов, 2001. 301 с.
15. Ahn I-P. Glufosinate ammonium-induced pathogen inhibition and defense responses culminate in disease protection in bar-transgenic rice // Plant Physiology. 2008. Vol. 146. N 1. P. 213–227.
16. Мишина Г.Н., Сережкина Г.В., Аветисян Т.В., Рябченко А.С., Андреев Л.Н. Особенности формирования гало в процессе патогенеза как ответная реакция эпидермальных клеток злаков на проникновение возбудителей мучнистой росы // Изв. РАН. Сер. биол. 2001. № 4. С. 424–430.
Review
For citations:
Avetisyan G.A., Avetisyan T.V. ANOMALIES OF THE EARLY STAGES OF DEVELOPMENT OF ERYSIPHE GRAMINIS TRITICI UNDER OXIDATIVE STRESS. Vestnik Moskovskogo universiteta. Seriya 16. Biologiya. 2017;72(2):70-74. (In Russ.)
Views:
344
Email this article 